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B9DMH1 (DDL_STACT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sca_1583
OrganismStaphylococcus carnosus (strain TM300) [Complete proteome] [HAMAP]
Taxonomic identifier396513 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000189745

Regions

Domain134 – 339206ATP-grasp
Nucleotide binding167 – 22256ATP By similarity

Sites

Metal binding2931Magnesium or manganese 1 By similarity
Metal binding3061Magnesium or manganese 1 By similarity
Metal binding3061Magnesium or manganese 2 By similarity
Metal binding3081Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B9DMH1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: C3C4A580B4037CB3

FASTA35639,874
        10         20         30         40         50         60 
MAKENICIIY GGKSAEHDVS ILTAQNVLNA INKDDYQVDI IYITNDGAWK KKDDITENVE 

        70         80         90        100        110        120 
DVDSLRLEEV EEGEISNLLT NSSSGQPYAA VFPLLHGPNG EDGTIQGLFE VLDLPYVGNG 

       130        140        150        160        170        180 
VLAASSTMDK LVMKQLFAHR GLPQLPYVSF LRSEYEKYQS NILKLVKDKL EFPVFVKPAN 

       190        200        210        220        230        240 
LGSSVGISKC NNEEELKSGI EEAFQFDRKL VIEQGIEARE IEVAVLGNDY PETTQPGEVV 

       250        260        270        280        290        300 
KDVAFYDYKS KYLDGKVQLS IPAELDSEVQ TTLRNMAAEA FKATDCSGLL RADFFVTEDN 

       310        320        330        340        350 
QIFINETNAM PGFTQYSMYP SLWENMGLSY ADLITKLIEL AKEKHVEKQK NKYKID 

« Hide

References

[1]"Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TM300.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM295250 Genomic DNA. Translation: CAL28488.1.
RefSeqYP_002634673.1. NC_012121.1.

3D structure databases

ProteinModelPortalB9DMH1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING396513.Sca_1583.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7551824.
KEGGsca:Sca_1583.
PATRIC19604013. VBIStaCar105558_1579.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAKLAFQYD.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycSCAR396513:GJ9G-1634-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_STACT
AccessionPrimary (citable) accession number: B9DMH1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways