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Protein

Biotin synthase

Gene

bioB

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi65Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi68Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi104Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi137Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi197Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi267Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSCAR396513:G1G1Q-2039-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Sca_2002
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000444 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003816561 – 336Biotin synthaseAdd BLAST336

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi396513.Sca_2002

Structurei

3D structure databases

ProteinModelPortaliB9DKN5
SMRiB9DKN5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B9DKN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTAHKIING YALTKQEAYD LYTDETIDTF TLLDEAYAVR KHYYGKKVKL
60 70 80 90 100
NMILNAKSGI CPEDCGYCGQ SRDMKRKDRY ALIPENDIIA GANAAADNQI
110 120 130 140 150
GTYCIVMSGR GPSDKEVDHI TESVRQIKEN HPQLKVCACL GLANEDQASR
160 170 180 190 200
LKEAGVDRYN HNLNTSENYH DTVVTTHTYE DRVKTVEIMK ENNISPCSGV
210 220 230 240 250
ICGMGETAQD RIDMAFALKE LDADSIPINF LHPIQGTKFG EMDELTPMKC
260 270 280 290 300
LRIIAIFRLV NPTKEIRIAG GREVNLQSLQ PFALLAANSI FVGDYLITGG
310 320 330
QPNTMDYRMI QDLGFEIDCS NLPEDTAEKI TETLEA
Length:336
Mass (Da):37,506
Last modified:March 24, 2009 - v1
Checksum:i554463E9C0BD5458
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM295250 Genomic DNA Translation: CAL28907.1
RefSeqiWP_015901243.1, NC_012121.1

Genome annotation databases

KEGGisca:SCA_2002

Similar proteinsi

Entry informationi

Entry nameiBIOB_STACT
AccessioniPrimary (citable) accession number: B9DKN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 25, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health