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B9DKN5

- BIOB_STACT

UniProt

B9DKN5 - BIOB_STACT

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Protein

Biotin synthase

Gene

bioB

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi104 – 1041Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi267 – 2671Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSCAR396513:GJ9G-2063-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Sca_2002
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000444: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Biotin synthasePRO_0000381656Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi396513.Sca_2002.

Structurei

3D structure databases

ProteinModelPortaliB9DKN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9DKN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTAHKIING YALTKQEAYD LYTDETIDTF TLLDEAYAVR KHYYGKKVKL
60 70 80 90 100
NMILNAKSGI CPEDCGYCGQ SRDMKRKDRY ALIPENDIIA GANAAADNQI
110 120 130 140 150
GTYCIVMSGR GPSDKEVDHI TESVRQIKEN HPQLKVCACL GLANEDQASR
160 170 180 190 200
LKEAGVDRYN HNLNTSENYH DTVVTTHTYE DRVKTVEIMK ENNISPCSGV
210 220 230 240 250
ICGMGETAQD RIDMAFALKE LDADSIPINF LHPIQGTKFG EMDELTPMKC
260 270 280 290 300
LRIIAIFRLV NPTKEIRIAG GREVNLQSLQ PFALLAANSI FVGDYLITGG
310 320 330
QPNTMDYRMI QDLGFEIDCS NLPEDTAEKI TETLEA
Length:336
Mass (Da):37,506
Last modified:March 24, 2009 - v1
Checksum:i554463E9C0BD5458
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28907.1.
RefSeqiYP_002635092.1. NC_012121.1.

Genome annotation databases

GeneIDi7550976.
KEGGisca:Sca_2002.
PATRICi19604876. VBIStaCar105558_2000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28907.1 .
RefSeqi YP_002635092.1. NC_012121.1.

3D structure databases

ProteinModelPortali B9DKN5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396513.Sca_2002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7550976.
KEGGi sca:Sca_2002.
PATRICi 19604876. VBIStaCar105558_2000.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SCAR396513:GJ9G-2063-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
    Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
    Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TM300.

Entry informationi

Entry nameiBIOB_STACT
AccessioniPrimary (citable) accession number: B9DKN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 1, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3