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B9DK67 (MDH_STACT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Sca_0338
OrganismStaphylococcus carnosus (strain TM300) [Complete proteome] [HAMAP]
Taxonomic identifier396513 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Malate dehydrogenase HAMAP-Rule MF_00487
PRO_1000191658

Regions

Nucleotide binding10 – 156NAD By similarity
Nucleotide binding121 – 1233NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site851Substrate By similarity
Binding site911Substrate By similarity
Binding site981NAD By similarity
Binding site1231Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B9DK67 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 68A032C142AC6569

FASTA31132,969
        10         20         30         40         50         60 
MAKNKIAIIG AGHTGSTLAF IIAERALADV VLLEIPKNEK PARGKALDIK ESGPILGFNG 

        70         80         90        100        110        120 
NVLGTSDYQD IAGADIVVIT AGAARKPGMS RDDLIQINEN VMAQVTEGIK KYAPESKIIV 

       130        140        150        160        170        180 
LTNPVDAMTY AVYKLSGFPK ERVLGQSGIL DTARYRTFVS EALNVAQTDV TGLVLGGHGD 

       190        200        210        220        230        240 
TMVPLLSTTM VGGVPLRELL AQDKIDAIVE RTRKGGAEIV GLLGNGSAYY APAAAIYEMA 

       250        260        270        280        290        300 
AAILNDERRL VPAITYLDGE YGFKDICLGV PTILGANGVE KVVEIELSDD EQQLRDSADA 

       310 
VEDVKSALKN K 

« Hide

References

[1]"Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TM300.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM295250 Genomic DNA. Translation: CAL27251.1.
RefSeqYP_002633436.1. NC_012121.1.

3D structure databases

ProteinModelPortalB9DK67.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING396513.Sca_0338.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7549988.
KEGGsca:Sca_0338.
PATRIC19601437. VBIStaCar105558_0337.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAYAYVDTD.
OrthoDBEOG6091FG.
ProtClustDBPRK06223.

Enzyme and pathway databases

BioCycSCAR396513:GJ9G-352-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_STACT
AccessionPrimary (citable) accession number: B9DK67
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families