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B9DIX8

- LIPA_STACT

UniProt

B9DIX8 - LIPA_STACT

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Protein
Lipoyl synthase
Gene
lipA, Sca_0528
Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi68 – 681Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi72 – 721Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi75 – 751Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSCAR396513:GJ9G-544-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Sca_0528
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000444: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Lipoyl synthaseUniRule annotation
PRO_1000124646Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi396513.Sca_0528.

Structurei

3D structure databases

ProteinModelPortaliB9DIX8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHTICQEA.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9DIX8-1 [UniParc]FASTAAdd to Basket

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MATKNEEILR KPDWLKIKLN TNENYTGLKK MMREKNLHTV CEEAKCPNIH    50
ECWGERRTAT FMILGAVCTR ACRFCAVKTG LPNELDLGEP ERVAESVELM 100
NLKHVVITAV ARDDLRDAGS NVYAETVRKV RERNPYTTIE ILPSDMGGDY 150
DALETLMASK PDILNHNIET VRRLTPRVRA RATYDRTLEF LRRSKELQPD 200
IPTKSSIMVG LGETIEELHE TMDDLRAADV DILTIGQYLQ PSRKHLKVQK 250
YYSPLEFGKL RKVAMEKGFK HCQAGPLVRS SYHADEQVNE AAKERQRIGD 300
EKLEAAKNEA 310
Length:310
Mass (Da):35,398
Last modified:March 24, 2009 - v1
Checksum:i70435221F62E9FCD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL27442.1.
RefSeqiYP_002633627.1. NC_012121.1.

Genome annotation databases

GeneIDi7550129.
KEGGisca:Sca_0528.
PATRICi19601826. VBIStaCar105558_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL27442.1 .
RefSeqi YP_002633627.1. NC_012121.1.

3D structure databases

ProteinModelPortali B9DIX8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396513.Sca_0528.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7550129.
KEGGi sca:Sca_0528.
PATRICi 19601826. VBIStaCar105558_0530.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HTICQEA.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

BioCyci SCAR396513:GJ9G-544-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
    Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
    Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TM300.

Entry informationi

Entry nameiLIPA_STACT
AccessioniPrimary (citable) accession number: B9DIX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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