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Protein

Thiamine pyrophosphokinase 1

Gene

TPK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (AXX17_At1g02080), Thiamine pyrophosphokinase (TPK1), Thiamine pyrophosphokinase (AXX17_At2g42340), Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase (AXX17_At2g42340)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: TAIR

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: GO_Central
  • thiamine metabolic process Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 1 (EC:2.7.6.2)
Short name:
AtTPK1
Alternative name(s):
Thiamine kinase 1
Gene namesi
Name:TPK1
Ordered Locus Names:At1g02880
ORF Names:F22D16.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G02880.
TAIRilocus:2024660. AT1G02880.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Tpk1 and tpk2 double mutants exhibit a seedling lethal phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239661 – 267Thiamine pyrophosphokinase 1Add BLAST267

Proteomic databases

PaxDbiB9DGU7.

PTM databases

iPTMnetiB9DGU7.

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.1 Publication

Gene expression databases

ExpressionAtlasiB9DGU7. baseline and differential.
GenevisibleiB9DGU7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02880.3.

Structurei

3D structure databases

ProteinModelPortaliB9DGU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
HOGENOMiHOG000180834.
KOiK00949.
OMAiESKMRFG.
OrthoDBiEOG09360JCF.
PhylomeDBiB9DGU7.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiView protein in InterPro
IPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR036371. TPK_B1-bd_sf.
IPR007371. TPK_catalytic.
IPR036759. TPK_catalytic_sf.
PfamiView protein in Pfam
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiView protein in SMART
SM00983. TPK_B1_binding. 1 hit.
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B9DGU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAMDVMIHS SSFLLPCDET STGTRYALVV LNQSLPRFTP LLWEHAKLRL
60 70 80 90 100
CADGGANRIY DELPLFFPNE DALAIRNRYK PDVIKGDMDS IRRDVLDFYI
110 120 130 140 150
NLGTKVIDES HDQDTTDLDK CILYIRHSTL NQETSGLQIL ATGALGGRFD
160 170 180 190 200
HEAGNLNVLY RYPDTRIVLL SDDCLIQLLP KTHRHEIHIQ SSLEGPHCGL
210 220 230 240 250
IPIGTPSAKT TTSGLQWDLS NTEMRFGGLI STSNLVKEEK ITVESDSDLL
260
WTISIKKTGL SIQDHTP
Length:267
Mass (Da):29,879
Last modified:May 3, 2011 - v1
Checksum:i9C1F54C74A7425EB
GO
Isoform 2 (identifier: B9DGU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: No experimental confirmation available.
Show »
Length:264
Mass (Da):29,590
Checksum:iA41EF949F059ADF3
GO
Isoform 3 (identifier: B9DGU7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: MSAMDVMIHS...PNEDALAIRN → MKKNLYF

Note: No experimental confirmation available.
Show »
Length:197
Mass (Da):22,167
Checksum:iB217DF40C4C33FAA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0532851 – 77MSAMD…LAIRN → MKKNLYF in isoform 3. 1 PublicationAdd BLAST77
Alternative sequenceiVSP_0532861 – 3Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009525 Genomic DNA. Translation: AAF02878.1.
CP002684 Genomic DNA. Translation: AEE27488.1.
CP002684 Genomic DNA. Translation: AEE27489.1.
CP002684 Genomic DNA. Translation: AEE27490.1.
CP002684 Genomic DNA. Translation: ANM59189.1.
AY058144 mRNA. Translation: AAL25560.1.
AY101539 mRNA. Translation: AAM26660.1.
AK317288 mRNA. Translation: BAH19964.1.
AY085584 mRNA. Translation: AAM62805.1.
PIRiB86159.
RefSeqiNP_001321569.1. NM_001331362.1. [B9DGU7-3]
NP_563669.1. NM_100169.2. [B9DGU7-2]
NP_849579.1. NM_179248.1. [B9DGU7-3]
NP_849580.1. NM_179249.3. [B9DGU7-1]
UniGeneiAt.20420.

Genome annotation databases

EnsemblPlantsiAT1G02880.1; AT1G02880.1; AT1G02880. [B9DGU7-3]
AT1G02880.2; AT1G02880.2; AT1G02880. [B9DGU7-2]
AT1G02880.3; AT1G02880.3; AT1G02880. [B9DGU7-1]
AT1G02880.5; AT1G02880.5; AT1G02880. [B9DGU7-3]
GeneIDi839303.
GrameneiAT1G02880.1; AT1G02880.1; AT1G02880.
AT1G02880.2; AT1G02880.2; AT1G02880.
AT1G02880.3; AT1G02880.3; AT1G02880.
AT1G02880.5; AT1G02880.5; AT1G02880.
KEGGiath:AT1G02880.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPK1_ARATH
AccessioniPrimary (citable) accession number: B9DGU7
Secondary accession number(s): Q93Z45, Q9SRY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 3, 2011
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families