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B9DFX7

- HMA8_ARATH

UniProt

B9DFX7 - HMA8_ARATH

Protein

Copper-transporting ATPase PAA2, chloroplastic

Gene

PAA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Mediates copper transfer across the chloroplast thylakoid membrane. Required for copper delivery into the thylakoids lumen, which is essential for the function of copper proteins.1 Publication

    Catalytic activityi

    ATP + H2O + Cu2+(Side 1) = ADP + phosphate + Cu2+(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi87 – 871CopperPROSITE-ProRule annotation
    Metal bindingi90 – 901CopperPROSITE-ProRule annotation
    Active sitei548 – 54814-aspartylphosphate intermediateBy similarity
    Metal bindingi762 – 7621MagnesiumPROSITE-ProRule annotation
    Metal bindingi766 – 7661MagnesiumPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi761 – 7688ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper-exporting ATPase activity Source: UniProtKB-EC
    3. copper ion transmembrane transporter activity Source: TAIR
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. copper ion transmembrane transport Source: GOC
    2. copper ion transport Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Copper transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G21930-MONOMER.
    ARA:GQT-886-MONOMER.
    MetaCyc:MONOMER-14496.

    Protein family/group databases

    TCDBi3.A.3.5.12. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Copper-transporting ATPase PAA2, chloroplastic (EC:3.6.3.4)
    Alternative name(s):
    Protein HEAVY METAL ATPASE 8
    Gene namesi
    Name:PAA2
    Synonyms:HMA8
    Ordered Locus Names:At5g21930
    ORF Names:F13M11, T6G21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G21930.

    Subcellular locationi

    Plastidchloroplast thylakoid membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: TAIR
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    Pathology & Biotechi

    Disruption phenotypei

    High-chlorophyll-fluorescence phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6565ChloroplastSequence AnalysisAdd
    BLAST
    Chaini66 – 883818Copper-transporting ATPase PAA2, chloroplasticPRO_0000416858Add
    BLAST

    Proteomic databases

    PaxDbiB9DFX7.
    PRIDEiB9DFX7.

    Expressioni

    Tissue specificityi

    Expressed in the shoots only and not in the roots.1 Publication

    Gene expression databases

    GenevestigatoriB9DFX7.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G21930.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliB9DFX7.
    SMRiB9DFX7. Positions 216-872.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei179 – 19921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei250 – 27021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei445 – 46521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei499 – 51921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei822 – 84221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei846 – 86621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 14771HMAPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HMA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2217.
    HOGENOMiHOG000250397.
    KOiK01533.
    OMAiHDMDNMH.
    PhylomeDBiB9DFX7.

    Family and domain databases

    Gene3Di2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR027256. Cation_transp_P-typ_ATPase_IB.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR006121. HeavyMe-assoc_HMA.
    [Graphical view]
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    PF00403. HMA. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SUPFAMiSSF55008. SSF55008. 1 hit.
    SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    PS50846. HMA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: B9DFX7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASNLLRFPL PPPSSLHIRP SKFLVNRCFP RLRRSRIRRH CSRPFFLVSN    50
    SVEISTQSFE STESSIESVK SITSDTPILL DVSGMMCGGC VARVKSVLMS 100
    DDRVASAVVN MLTETAAVKF KPEVEVTADT AESLAKRLTE SGFEAKRRVS 150
    GMGVAENVKK WKEMVSKKED LLVKSRNRVA FAWTLVALCC GSHTSHILHS 200
    LGIHIAHGGI WDLLHNSYVK GGLAVGALLG PGRELLFDGI KAFGKRSPNM 250
    NSLVGLGSMA AFSISLISLV NPELEWDASF FDEPVMLLGF VLLGRSLEER 300
    AKLQASTDMN ELLSLISTQS RLVITSSDNN TPVDSVLSSD SICINVSVDD 350
    IRVGDSLLVL PGETFPVDGS VLAGRSVVDE SMLTGESLPV FKEEGCSVSA 400
    GTINWDGPLR IKASSTGSNS TISKIVRMVE DAQGNAAPVQ RLADAIAGPF 450
    VYTIMSLSAM TFAFWYYVGS HIFPDVLLND IAGPDGDALA LSLKLAVDVL 500
    VVSCPCALGL ATPTAILIGT SLGAKRGYLI RGGDVLERLA SIDCVALDKT 550
    GTLTEGRPVV SGVASLGYEE QEVLKMAAAV EKTATHPIAK AIVNEAESLN 600
    LKTPETRGQL TEPGFGTLAE IDGRFVAVGS LEWVSDRFLK KNDSSDMVKL 650
    ESLLDHKLSN TSSTSRYSKT VVYVGREGEG IIGAIAISDC LRQDAEFTVA 700
    RLQEKGIKTV LLSGDREGAV ATVAKNVGIK SESTNYSLSP EKKFEFISNL 750
    QSSGHRVAMV GDGINDAPSL AQADVGIALK IEAQENAASN AASVILVRNK 800
    LSHVVDALSL AQATMSKVYQ NLAWAIAYNV ISIPIAAGVL LPQYDFAMTP 850
    SLSGGLMALS SIFVVSNSLL LQLHKSETSK NSL 883
    Length:883
    Mass (Da):94,260
    Last modified:March 24, 2009 - v1
    Checksum:iF5E365AB6B7A90C3
    GO

    Sequence cautioni

    The sequence AAO73891.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAC34486.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti312 – 3121L → P in AAP55720. (PubMed:15772282)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY297817 mRNA. Translation: AAP55720.1.
    AC140977 Genomic DNA. Translation: AAO73891.1. Sequence problems.
    AL589883 Genomic DNA. Translation: CAC34486.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92956.1.
    CP002688 Genomic DNA. Translation: AED92957.1.
    AK316941 mRNA. Translation: BAH19644.1.
    RefSeqiNP_001031920.1. NM_001036843.1. [B9DFX7-1]
    NP_680181.2. NM_147876.4. [B9DFX7-1]
    UniGeneiAt.44341.

    Genome annotation databases

    EnsemblPlantsiAT5G21930.1; AT5G21930.1; AT5G21930. [B9DFX7-1]
    AT5G21930.2; AT5G21930.2; AT5G21930. [B9DFX7-1]
    GeneIDi832253.
    KEGGiath:AT5G21930.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY297817 mRNA. Translation: AAP55720.1 .
    AC140977 Genomic DNA. Translation: AAO73891.1 . Sequence problems.
    AL589883 Genomic DNA. Translation: CAC34486.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92956.1 .
    CP002688 Genomic DNA. Translation: AED92957.1 .
    AK316941 mRNA. Translation: BAH19644.1 .
    RefSeqi NP_001031920.1. NM_001036843.1. [B9DFX7-1 ]
    NP_680181.2. NM_147876.4. [B9DFX7-1 ]
    UniGenei At.44341.

    3D structure databases

    ProteinModelPortali B9DFX7.
    SMRi B9DFX7. Positions 216-872.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G21930.2-P.

    Protein family/group databases

    TCDBi 3.A.3.5.12. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    PaxDbi B9DFX7.
    PRIDEi B9DFX7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G21930.1 ; AT5G21930.1 ; AT5G21930 . [B9DFX7-1 ]
    AT5G21930.2 ; AT5G21930.2 ; AT5G21930 . [B9DFX7-1 ]
    GeneIDi 832253.
    KEGGi ath:AT5G21930.

    Organism-specific databases

    TAIRi AT5G21930.

    Phylogenomic databases

    eggNOGi COG2217.
    HOGENOMi HOG000250397.
    KOi K01533.
    OMAi HDMDNMH.
    PhylomeDBi B9DFX7.

    Enzyme and pathway databases

    BioCyci ARA:AT5G21930-MONOMER.
    ARA:GQT-886-MONOMER.
    MetaCyc:MONOMER-14496.

    Gene expression databases

    Genevestigatori B9DFX7.

    Family and domain databases

    Gene3Di 2.70.150.10. 1 hit.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProi IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR027256. Cation_transp_P-typ_ATPase_IB.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR006121. HeavyMe-assoc_HMA.
    [Graphical view ]
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    PF00403. HMA. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SUPFAMi SSF55008. SSF55008. 1 hit.
    SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01525. ATPase-IB_hvy. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    PS50846. HMA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts."
      Abdel-Ghany S.E., Muller-Moule P., Niyogi K.K., Pilon M., Shikanai T.
      Plant Cell 17:1233-1251(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiHMA8_ARATH
    AccessioniPrimary (citable) accession number: B9DFX7
    Secondary accession number(s): Q7Y051, Q9C594
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3