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Protein

Copper-transporting ATPase PAA2, chloroplastic

Gene

PAA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates copper transfer across the chloroplast thylakoid membrane. Required for copper delivery into the thylakoids lumen, which is essential for the function of copper proteins.1 Publication

Catalytic activityi

ATP + H2O + Cu2+(Side 1) = ADP + phosphate + Cu2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871CopperPROSITE-ProRule annotation
Metal bindingi90 – 901CopperPROSITE-ProRule annotation
Active sitei548 – 54814-aspartylphosphate intermediateBy similarity
Metal bindingi762 – 7621MagnesiumPROSITE-ProRule annotation
Metal bindingi766 – 7661MagnesiumPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi761 – 7688ATPSequence Analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper-exporting ATPase activity Source: UniProtKB-EC
  • copper ion transmembrane transporter activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • copper ion transmembrane transport Source: GOC
  • copper ion transport Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G21930-MONOMER.
ARA:GQT-886-MONOMER.
MetaCyc:MONOMER-14496.
BRENDAi3.6.3.4. 399.

Protein family/group databases

TCDBi3.A.3.5.12. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase PAA2, chloroplastic (EC:3.6.3.4)
Alternative name(s):
Protein HEAVY METAL ATPASE 8
Gene namesi
Name:PAA2
Synonyms:HMA8
Ordered Locus Names:At5g21930
ORF Names:F13M11, T6G21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G21930.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei179 – 19921HelicalSequence AnalysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence AnalysisAdd
BLAST
Transmembranei250 – 27021HelicalSequence AnalysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence AnalysisAdd
BLAST
Transmembranei445 – 46521HelicalSequence AnalysisAdd
BLAST
Transmembranei499 – 51921HelicalSequence AnalysisAdd
BLAST
Transmembranei822 – 84221HelicalSequence AnalysisAdd
BLAST
Transmembranei846 – 86621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • chloroplast thylakoid membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

High-chlorophyll-fluorescence phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6565ChloroplastSequence AnalysisAdd
BLAST
Chaini66 – 883818Copper-transporting ATPase PAA2, chloroplasticPRO_0000416858Add
BLAST

Proteomic databases

PaxDbiB9DFX7.
PRIDEiB9DFX7.

Expressioni

Tissue specificityi

Expressed in the shoots only and not in the roots.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G21930.1.

Structurei

3D structure databases

ProteinModelPortaliB9DFX7.
SMRiB9DFX7. Positions 216-872.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 14771HMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2217.
HOGENOMiHOG000250397.
InParanoidiB9DFX7.
KOiK01533.
OMAiDEMMSEH.
PhylomeDBiB9DFX7.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HeavyMe-assoc_HMA.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: B9DFX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASNLLRFPL PPPSSLHIRP SKFLVNRCFP RLRRSRIRRH CSRPFFLVSN
60 70 80 90 100
SVEISTQSFE STESSIESVK SITSDTPILL DVSGMMCGGC VARVKSVLMS
110 120 130 140 150
DDRVASAVVN MLTETAAVKF KPEVEVTADT AESLAKRLTE SGFEAKRRVS
160 170 180 190 200
GMGVAENVKK WKEMVSKKED LLVKSRNRVA FAWTLVALCC GSHTSHILHS
210 220 230 240 250
LGIHIAHGGI WDLLHNSYVK GGLAVGALLG PGRELLFDGI KAFGKRSPNM
260 270 280 290 300
NSLVGLGSMA AFSISLISLV NPELEWDASF FDEPVMLLGF VLLGRSLEER
310 320 330 340 350
AKLQASTDMN ELLSLISTQS RLVITSSDNN TPVDSVLSSD SICINVSVDD
360 370 380 390 400
IRVGDSLLVL PGETFPVDGS VLAGRSVVDE SMLTGESLPV FKEEGCSVSA
410 420 430 440 450
GTINWDGPLR IKASSTGSNS TISKIVRMVE DAQGNAAPVQ RLADAIAGPF
460 470 480 490 500
VYTIMSLSAM TFAFWYYVGS HIFPDVLLND IAGPDGDALA LSLKLAVDVL
510 520 530 540 550
VVSCPCALGL ATPTAILIGT SLGAKRGYLI RGGDVLERLA SIDCVALDKT
560 570 580 590 600
GTLTEGRPVV SGVASLGYEE QEVLKMAAAV EKTATHPIAK AIVNEAESLN
610 620 630 640 650
LKTPETRGQL TEPGFGTLAE IDGRFVAVGS LEWVSDRFLK KNDSSDMVKL
660 670 680 690 700
ESLLDHKLSN TSSTSRYSKT VVYVGREGEG IIGAIAISDC LRQDAEFTVA
710 720 730 740 750
RLQEKGIKTV LLSGDREGAV ATVAKNVGIK SESTNYSLSP EKKFEFISNL
760 770 780 790 800
QSSGHRVAMV GDGINDAPSL AQADVGIALK IEAQENAASN AASVILVRNK
810 820 830 840 850
LSHVVDALSL AQATMSKVYQ NLAWAIAYNV ISIPIAAGVL LPQYDFAMTP
860 870 880
SLSGGLMALS SIFVVSNSLL LQLHKSETSK NSL
Length:883
Mass (Da):94,260
Last modified:March 24, 2009 - v1
Checksum:iF5E365AB6B7A90C3
GO

Sequence cautioni

The sequence AAO73891.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAC34486.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti312 – 3121L → P in AAP55720 (PubMed:15772282).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297817 mRNA. Translation: AAP55720.1.
AC140977 Genomic DNA. Translation: AAO73891.1. Sequence problems.
AL589883 Genomic DNA. Translation: CAC34486.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92956.1.
CP002688 Genomic DNA. Translation: AED92957.1.
AK316941 mRNA. Translation: BAH19644.1.
RefSeqiNP_001031920.1. NM_001036843.1. [B9DFX7-1]
NP_680181.2. NM_147876.4. [B9DFX7-1]
UniGeneiAt.44341.

Genome annotation databases

EnsemblPlantsiAT5G21930.1; AT5G21930.1; AT5G21930. [B9DFX7-1]
AT5G21930.2; AT5G21930.2; AT5G21930. [B9DFX7-1]
GeneIDi832253.
KEGGiath:AT5G21930.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297817 mRNA. Translation: AAP55720.1.
AC140977 Genomic DNA. Translation: AAO73891.1. Sequence problems.
AL589883 Genomic DNA. Translation: CAC34486.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92956.1.
CP002688 Genomic DNA. Translation: AED92957.1.
AK316941 mRNA. Translation: BAH19644.1.
RefSeqiNP_001031920.1. NM_001036843.1. [B9DFX7-1]
NP_680181.2. NM_147876.4. [B9DFX7-1]
UniGeneiAt.44341.

3D structure databases

ProteinModelPortaliB9DFX7.
SMRiB9DFX7. Positions 216-872.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G21930.1.

Protein family/group databases

TCDBi3.A.3.5.12. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiB9DFX7.
PRIDEiB9DFX7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G21930.1; AT5G21930.1; AT5G21930. [B9DFX7-1]
AT5G21930.2; AT5G21930.2; AT5G21930. [B9DFX7-1]
GeneIDi832253.
KEGGiath:AT5G21930.

Organism-specific databases

TAIRiAT5G21930.

Phylogenomic databases

eggNOGiCOG2217.
HOGENOMiHOG000250397.
InParanoidiB9DFX7.
KOiK01533.
OMAiDEMMSEH.
PhylomeDBiB9DFX7.

Enzyme and pathway databases

BioCyciARA:AT5G21930-MONOMER.
ARA:GQT-886-MONOMER.
MetaCyc:MONOMER-14496.
BRENDAi3.6.3.4. 399.

Miscellaneous databases

PROiB9DFX7.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HeavyMe-assoc_HMA.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts."
    Abdel-Ghany S.E., Muller-Moule P., Niyogi K.K., Pilon M., Shikanai T.
    Plant Cell 17:1233-1251(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiHMA8_ARATH
AccessioniPrimary (citable) accession number: B9DFX7
Secondary accession number(s): Q7Y051, Q9C594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 24, 2009
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.