B9DFU2 (MAX1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 711A1
EC=1.14.-.-

Alternative name(s):
Protein MORE AXILLARY BRANCHES 1

Gene names

Name:	CYP711A1
Synonyms:	MAX1
Ordered Locus Names:	At2g26170
ORF Names:	T19L18.2, T1D16.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts downstream of CCD7/MAX3 and CCD8/MAX4 in strigolactone signaling pathway and may be implicated in synthesis of carotenoid-derived branch regulators. Acts as a positive regulator of the flavonoid pathway in the late vegetative stage plant. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Ref.5 Ref.6
Cofactor	Heme group By similarity.
Subcellular location	Membrane Potential; Single-pass membrane protein.
Tissue specificity	Expressed in the vascular cylinder (including the pericycle) in roots, the cambial region of vascular bundles in inflorescence stems and the vascular junctions of axillary regions in leaves and flowers. Ref.5
Disruption phenotype	Increased shoot branching. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Miscellaneous	The branching phenotypes of the max1, ccd7/max3 and ccd8/max4 mutants can be rescued by exogenous treatment with the synthetic strigolactone analogs GR24 and 4BD (Ref.9).
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAL91286.1 differs from that shown. Reason: Frameshift at position 102.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	auxin polar transport Inferred from mutant phenotype PubMed 16546078. Source: TAIR carotenoid biosynthetic process Traceable author statement Ref.5. Source: TAIR positive regulation of flavonoid biosynthetic process Inferred from expression pattern Ref.6. Source: TAIR regulation of meristem structural organization Inferred from mutant phenotype PubMed 11874909. Source: TAIR secondary shoot formation Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B9DFU2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B9DFU2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Cytochrome P450 711A1		PRO_0000422062
Regions
	Transmembrane	14 – 34	21	Helical; Potential
Sites
	Metal binding	467	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	1 – 83	83	Missing in isoform 2.		VSP_046323

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 3, 2011. Version 1. Checksum: 77E46CD65838398F	FASTA	522	59,425
	10 20 30 40 50 60 MKTQHQWWEV LDPFLTQHEA LIAFLTFAAV VIVIYLYRPS WSVCNVPGPT AMPLVGHLPL 70 80 90 100 110 120 MAKYGPDVFS VLAKQYGPIF RFQMGRQPLI IIAEAELCRE VGIKKFKDLP NRSIPSPISA 130 140 150 160 170 180 SPLHKKGLFF TRDKRWSKMR NTILSLYQPS HLTSLIPTMH SFITSATHNL DSKPRDIVFS 190 200 210 220 230 240 NLFLKLTTDI IGQAAFGVDF GLSGKKPIKD VEVTDFINQH VYSTTQLKMD LSGSLSIILG 250 260 270 280 290 300 LLIPILQEPF RQVLKRIPGT MDWRVEKTNA RLSGQLNEIV SKRAKEAETD SKDFLSLILK 310 320 330 340 350 360 ARESDPFAKN IFTSDYISAV TYEHLLAGSA TTAFTLSSVL YLVSGHLDVE KRLLQEIDGF 370 380 390 400 410 420 GNRDLIPTAH DLQHKFPYLD QVIKEAMRFY MVSPLVARET AKEVEIGGYL LPKGTWVWLA 430 440 450 460 470 480 LGVLAKDPKN FPEPEKFKPE RFDPNGEEEK HRHPYAFIPF GIGPRACVGQ RFALQEIKLT 490 500 510 520 LLHLYRNYIF RHSLEMEIPL QLDYGIILSF KNGVKLRTIK RF
	Isoform 2 [UniParc]. Checksum: 68C5335CC020A2AA	FASTA	439	49,951
	10 20 30 40 50 60 MGRQPLIIIA EAELCREVGI KKFKDLPNRS IPSPISASPL HKKGLFFTRD KRWSKMRNTI 70 80 90 100 110 120 LSLYQPSHLT SLIPTMHSFI TSATHNLDSK PRDIVFSNLF LKLTTDIIGQ AAFGVDFGLS 130 140 150 160 170 180 GKKPIKDVEV TDFINQHVYS TTQLKMDLSG SLSIILGLLI PILQEPFRQV LKRIPGTMDW 190 200 210 220 230 240 RVEKTNARLS GQLNEIVSKR AKEAETDSKD FLSLILKARE SDPFAKNIFT SDYISAVTYE 250 260 270 280 290 300 HLLAGSATTA FTLSSVLYLV SGHLDVEKRL LQEIDGFGNR DLIPTAHDLQ HKFPYLDQVI 310 320 330 340 350 360 KEAMRFYMVS PLVARETAKE VEIGGYLLPK GTWVWLALGV LAKDPKNFPE PEKFKPERFD 370 380 390 400 410 420 PNGEEEKHRH PYAFIPFGIG PRACVGQRFA LQEIKLTLLH LYRNYIFRHS LEMEIPLQLD 430 YGIILSFKNG VKLRTIKRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone." Booker J., Sieberer T., Wright W., Williamson L., Willett B., Stirnberg P., Turnbull C., Srinivasan M., Goddard P., Leyser O. Dev. Cell 8:443-449(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"MAX1, a regulator of the flavonoid pathway, controls vegetative axillary bud outgrowth in Arabidopsis." Lazar G., Goodman H.M. Proc. Natl. Acad. Sci. U.S.A. 103:472-476(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Strigolactones enhance competition between shoot branches by dampening auxin transport." Crawford S., Shinohara N., Sieberer T., Williamson L., George G., Hepworth J., Mueller D., Domagalska M.A., Leyser O. Development 137:2905-2913(2010) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[8]	"Strigolactones are transported through the xylem and play a key role in shoot architectural response to phosphate deficiency in nonarbuscular mycorrhizal host Arabidopsis." Kohlen W., Charnikhova T., Liu Q., Bours R., Domagalska M.A., Beguerie S., Verstappen F., Leyser O., Bouwmeester H., Ruyter-Spira C. Plant Physiol. 155:974-987(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[9]	"Selective mimics of strigolactone actions and their potential use for controlling damage caused by root parasitic weeds." Fukui K., Ito S., Asami T. Mol. Plant 6:88-99(2013) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004484 Genomic DNA. Translation: AAC14532.2. AC004747 Genomic DNA. Translation: AAM15001.1. CP002685 Genomic DNA. Translation: AEC07803.1. CP002685 Genomic DNA. Translation: AEC07804.1. AY054283 mRNA. Translation: AAL06942.1. AY090384 mRNA. Translation: AAL91286.1. Frameshift. AK316903 mRNA. Translation: BAH19609.1.
IPI	IPI00531292.
PIR	T02607.
RefSeq	NP_565617.2. NM_128175.3. NP_850074.1. NM_179743.2.
UniGene	At.26382. At.48520.
3D structure databases
ProteinModelPortal	B9DFU2.
SMR	B9DFU2. Positions 46-521.
ModBase	Search...
Proteomic databases
PRIDE	B9DFU2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G26170.1; AT2G26170.1; AT2G26170.
GeneID	817157.
KEGG	ath:AT2G26170.
Organism-specific databases
GeneFarm	1383. 94.
TAIR	At2g26170.
Phylogenomic databases
OMA	PLMAKYG.
PhylomeDB	B9DFU2.
ProtClustDB	CLSN2918099.
Gene expression databases
ArrayExpress	B9DFU2.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAX1_ARATH

Accession

Primary (citable) accession number: B9DFU2
Secondary accession number(s): O64853, Q940L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2013
Last sequence update:	May 3, 2011
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program