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B9DFG5 (PTI13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PTI1-like tyrosine-protein kinase 3

Short name=PTI1-3
EC=2.7.10.2
Gene names
Name:PTI13
Ordered Locus Names:At3g59350
ORF Names:F25L23.210
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with OXI1. Ref.5

Subcellular location

Cell membrane; Peripheral membrane protein Ref.6.

Post-translational modification

Phosphorylated by OXI1. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAB91605.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B9DFG5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B9DFG5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408PTI1-like tyrosine-protein kinase 3
PRO_0000403324

Regions

Domain113 – 395283Protein kinase
Nucleotide binding119 – 1279ATP By similarity

Sites

Active site2451Proton acceptor By similarity
Binding site1411ATP By similarity

Natural variations

Alternative sequence1 – 4242Missing in isoform 2.
VSP_040369

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 57AF0E1D1E5B0F8C

FASTA40845,658
        10         20         30         40         50         60 
MYPMDSDYHR RGLVANDRSP AQFVRLDKPR AVDDLYIGKR EKMRRWLCCA CHVEEPYHSS 

        70         80         90        100        110        120 
ENEHLRSPKH HNDFGHHTRK PQAAVKPDAL KEPPSIDVPA LSLDELKEKT DNFGSKSLIG 

       130        140        150        160        170        180 
EGSYGRAYYA TLKDGKAVAV KKLDNAAEPE SNVEFLTQVS RVSKLKHDNF VELFGYCVEG 

       190        200        210        220        230        240 
NFRILAYEFA TMGSLHDILH GRKGVQGAQP GPTLDWIQRV RIAVDAARGL EYLHEKVQPA 

       250        260        270        280        290        300 
VIHRDIRSSN VLLFEDFKAK IADFNLSNQS PDMAARLHST RVLGTFGYHA PEYAMTGQLT 

       310        320        330        340        350        360 
QKSDVYSFGV VLLELLTGRK PVDHTMPRGQ QSLVTWATPR LSEDKVKQCV DPKLKGEYPP 

       370        380        390        400 
KAVAKLAAVA ALCVQYESEF RPNMSIVVKA LQPLLRSSTA AAVPVQEA 

« Hide

Isoform 2 [UniParc].

Checksum: 74A99836337DCEF7
Show »

FASTA36640,695

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidative stress signaling pathways downstream of PDK1 and OXI1."
Anthony R.G., Khan S., Costa J., Pais M.S., Boegre L.
J. Biol. Chem. 281:37536-37546(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OXI1, PHOSPHORYLATION BY OXI1.
[6]"A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome."
Marmagne A., Ferro M., Meinnel T., Bruley C., Kuhn L., Garin J., Barbier-Brygoo H., Ephritikhine G.
Mol. Cell. Proteomics 6:1980-1996(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL356014 Genomic DNA. Translation: CAB91605.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79908.1.
CP002686 Genomic DNA. Translation: AEE79909.1.
CP002686 Genomic DNA. Translation: AEE79910.1.
AY054639 mRNA. Translation: AAK96830.1.
BT008449 mRNA. Translation: AAP37808.1.
AK316761 mRNA. Translation: BAH19482.1.
AK317695 mRNA. Translation: BAH20354.1.
PIRT49003.
RefSeqNP_001030893.1. NM_001035816.1.
NP_567082.2. NM_115797.5.
NP_850720.1. NM_180389.2.
UniGeneAt.1517.

3D structure databases

ProteinModelPortalB9DFG5.
SMRB9DFG5. Positions 105-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10419. 3 interactions.
IntActB9DFG5. 2 interactions.
STRING3702.AT3G59350.1-P.

Proteomic databases

PaxDbB9DFG5.
PRIDEB9DFG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G59350.1; AT3G59350.1; AT3G59350. [B9DFG5-1]
AT3G59350.3; AT3G59350.3; AT3G59350. [B9DFG5-1]
GeneID825104.
KEGGath:AT3G59350.

Organism-specific databases

GeneFarm1786. 139.
TAIRAT3G59350.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000116550.
InParanoidQ9LX36.
OMAKLKHDNF.
PhylomeDBB9DFG5.
ProtClustDBCLSN2680456.

Enzyme and pathway databases

BioCycARA:AT3G59350-MONOMER.
ARA:GQT-2063-MONOMER.

Gene expression databases

GenevestigatorB9DFG5.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTI13_ARATH
AccessionPrimary (citable) accession number: B9DFG5
Secondary accession number(s): B9DHY7, Q940H1, Q9LX36
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names