B9DFG5 (PTI13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTI1-like tyrosine-protein kinase 3
Short name=PTI1-3
EC=2.7.10.2

Gene names

Name:	PTI13
Ordered Locus Names:	At3g59350
ORF Names:	F25L23.210

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with OXI1. Ref.5
Subcellular location	Cell membrane; Peripheral membrane protein Ref.6.
Post-translational modification	Phosphorylated by OXI1. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence CAB91605.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from direct assay Ref.6PubMed 22923678. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: B9DFG5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: B9DFG5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	PTI1-like tyrosine-protein kinase 3		PRO_0000403324
Regions
	Domain	113 – 395	283	Protein kinase
	Nucleotide binding	119 – 127	9	ATP By similarity
Sites
	Active site	245	1	Proton acceptor By similarity
	Binding site	141	1	ATP By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 42	42	Missing in isoform 2.		VSP_040369

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 57AF0E1D1E5B0F8C	FASTA	408	45,658
	10 20 30 40 50 60 MYPMDSDYHR RGLVANDRSP AQFVRLDKPR AVDDLYIGKR EKMRRWLCCA CHVEEPYHSS 70 80 90 100 110 120 ENEHLRSPKH HNDFGHHTRK PQAAVKPDAL KEPPSIDVPA LSLDELKEKT DNFGSKSLIG 130 140 150 160 170 180 EGSYGRAYYA TLKDGKAVAV KKLDNAAEPE SNVEFLTQVS RVSKLKHDNF VELFGYCVEG 190 200 210 220 230 240 NFRILAYEFA TMGSLHDILH GRKGVQGAQP GPTLDWIQRV RIAVDAARGL EYLHEKVQPA 250 260 270 280 290 300 VIHRDIRSSN VLLFEDFKAK IADFNLSNQS PDMAARLHST RVLGTFGYHA PEYAMTGQLT 310 320 330 340 350 360 QKSDVYSFGV VLLELLTGRK PVDHTMPRGQ QSLVTWATPR LSEDKVKQCV DPKLKGEYPP 370 380 390 400 KAVAKLAAVA ALCVQYESEF RPNMSIVVKA LQPLLRSSTA AAVPVQEA
	Isoform 2 [UniParc]. Checksum: 74A99836337DCEF7	FASTA	366	40,695
	10 20 30 40 50 60 MRRWLCCACH VEEPYHSSEN EHLRSPKHHN DFGHHTRKPQ AAVKPDALKE PPSIDVPALS 70 80 90 100 110 120 LDELKEKTDN FGSKSLIGEG SYGRAYYATL KDGKAVAVKK LDNAAEPESN VEFLTQVSRV 130 140 150 160 170 180 SKLKHDNFVE LFGYCVEGNF RILAYEFATM GSLHDILHGR KGVQGAQPGP TLDWIQRVRI 190 200 210 220 230 240 AVDAARGLEY LHEKVQPAVI HRDIRSSNVL LFEDFKAKIA DFNLSNQSPD MAARLHSTRV 250 260 270 280 290 300 LGTFGYHAPE YAMTGQLTQK SDVYSFGVVL LELLTGRKPV DHTMPRGQQS LVTWATPRLS 310 320 330 340 350 360 EDKVKQCVDP KLKGEYPPKA VAKLAAVAAL CVQYESEFRP NMSIVVKALQ PLLRSSTAAA VPVQEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidative stress signaling pathways downstream of PDK1 and OXI1." Anthony R.G., Khan S., Costa J., Pais M.S., Boegre L. J. Biol. Chem. 281:37536-37546(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OXI1, PHOSPHORYLATION BY OXI1.
[6]	"A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome." Marmagne A., Ferro M., Meinnel T., Bruley C., Kuhn L., Garin J., Barbier-Brygoo H., Ephritikhine G. Mol. Cell. Proteomics 6:1980-1996(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

PlantP kinase Classification PPC

Arabidopsis protein tyrosine kinases

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL356014 Genomic DNA. Translation: CAB91605.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE79908.1. CP002686 Genomic DNA. Translation: AEE79909.1. CP002686 Genomic DNA. Translation: AEE79910.1. AY054639 mRNA. Translation: AAK96830.1. BT008449 mRNA. Translation: AAP37808.1. AK316761 mRNA. Translation: BAH19482.1. AK317695 mRNA. Translation: BAH20354.1.
IPI	IPI00518930. IPI00522951.
PIR	T49003.
RefSeq	NP_001030893.1. NM_001035816.1. NP_567082.2. NM_115797.5. NP_850720.1. NM_180389.2.
UniGene	At.1517.
3D structure databases
ProteinModelPortal	B9DFG5.
SMR	B9DFG5. Positions 81-396.
ModBase	Search...
Protein-protein interaction databases
IntAct	B9DFG5. 2 interactions.
STRING	3702.AT3G59350.1-P.
Proteomic databases
PaxDb	B9DFG5.
PRIDE	B9DFG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G59350.1; AT3G59350.1; AT3G59350. AT3G59350.3; AT3G59350.3; AT3G59350.
GeneID	825104.
KEGG	ath:AT3G59350.
Organism-specific databases
GeneFarm	1786. 139.
TAIR	At3g59350.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116550.
InParanoid	Q9LX36.
OMA	KLKHDNF.
PhylomeDB	B9DFG5.
ProtClustDB	CLSN2680456.
Gene expression databases
Genevestigator	B9DFG5.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTI13_ARATH

Accession

Primary (citable) accession number: B9DFG5
Secondary accession number(s): B9DHY7, Q940H1, Q9LX36

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program