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Protein

Queuine tRNA-ribosyltransferase subunit QTRTD1

Gene

Qtrtd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation1 Publication

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation1 Publication

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei272 – 2721NucleophileUniRule annotation
Metal bindingi351 – 3511ZincUniRule annotation
Metal bindingi353 – 3531ZincUniRule annotation
Metal bindingi356 – 3561ZincUniRule annotation
Metal bindingi382 – 3821ZincUniRule annotation

GO - Molecular functioni

  • queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • queuosine biosynthetic process Source: UniProtKB
  • tRNA modification Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase subunit QTRTD1UniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Queuine tRNA-ribosyltransferase domain-containing protein 1UniRule annotation
Gene namesi
Name:Qtrtd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1922194. Qtrtd1.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Mitochondrion UniRule annotation1 Publication

  • Note: May associate with the mitochondrion outer membrane.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Queuine tRNA-ribosyltransferase subunit QTRTD1PRO_0000383924Add
BLAST

Proteomic databases

EPDiB8ZXI1.
MaxQBiB8ZXI1.
PaxDbiB8ZXI1.
PeptideAtlasiB8ZXI1.
PRIDEiB8ZXI1.

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, liver, lung, skeletal muscle, spleen and testis.1 Publication

Interactioni

Subunit structurei

Interacts with QTRT1.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023387.

Structurei

3D structure databases

ProteinModelPortaliB8ZXI1.
SMRiB8ZXI1. Positions 119-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3909. Eukaryota.
COG0343. LUCA.
InParanoidiB8ZXI1.
KOiK15407.
OMAiGCRLGKI.
PhylomeDBiB8ZXI1.
TreeFamiTF317105.

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRD1. 1 hit.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B8ZXI1-1) [UniParc]FASTAAdd to basket
Also known as: Qv1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLSLIKVVN GCRLGKIQNL GKAGDCTVDI PGCLLYTRTG SAPHLTHQTL
60 70 80 90 100
RNIHGVPGIA QLTLSSLAEH HEVLAEYKKG VGSFIGMPES LFYCSLHDPV
110 120 130 140 150
TPGPAGYVTS KSVSVWGFGG RVEMTVSKFM AIQEALQPDW FQCLSDGEAS
160 170 180 190 200
CAETTSIKRA RKSVDRSLLF LDSCLRLQEE SEVLQKSVII GVIEGGDVME
210 220 230 240 250
ERLRSARETA KRPVGGFLLD GFQGDPAVTE TRLHLLSSVT AELPEDKPRL
260 270 280 290 300
ICGVSRPDEV LECIERGVDL FESFFPYQVT ERGCALTFTF DCQLNPEETL
310 320 330 340 350
LQQNGIQEKI KGLDQAKKIE ATGCNQEMTS FEINLKEKKY QEDFDPLVRG
360 370 380 390 400
CSCYCCKNHT RAYIHHLLMT NELLAGVLLM MHNFEHYFGF FCSIREALKN
410
DTLAQLKELI CRQMF
Note: Major isoform in adult tissues.
Length:415
Mass (Da):46,288
Last modified:September 22, 2009 - v2
Checksum:i4E95A4FC8FFBAB22
GO
Isoform 2 (identifier: B8ZXI1-2) [UniParc]FASTAAdd to basket
Also known as: Qv0

The sequence of this isoform differs from the canonical sequence as follows:
     112-182: Missing.

Show »
Length:344
Mass (Da):38,344
Checksum:iC106985E465E662F
GO

Sequence cautioni

The sequence CAP19252 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAX17683 differs from that shown. Reason: Erroneous initiation. Curated
The sequence EDK98022 differs from that shown. Reason: Erroneous initiation. Curated
The sequence EDK98023 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 18271Missing in isoform 2. 1 PublicationVSP_038046Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM985972 mRNA. Translation: CAX17683.1. Different initiation.
CT025520 Genomic DNA. Translation: CAP19252.1. Different initiation.
CH466521 Genomic DNA. Translation: EDK98022.1. Different initiation.
CH466521 Genomic DNA. Translation: EDK98023.1. Different initiation.
BC083089 mRNA. Translation: AAH83089.1.
RefSeqiNP_083404.2. NM_029128.2.
XP_006521731.1. XM_006521668.1.
XP_006521733.1. XM_006521670.2.
UniGeneiMm.275354.

Genome annotation databases

GeneIDi106248.
KEGGimmu:106248.
UCSCiuc007zgl.1. mouse. [B8ZXI1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM985972 mRNA. Translation: CAX17683.1. Different initiation.
CT025520 Genomic DNA. Translation: CAP19252.1. Different initiation.
CH466521 Genomic DNA. Translation: EDK98022.1. Different initiation.
CH466521 Genomic DNA. Translation: EDK98023.1. Different initiation.
BC083089 mRNA. Translation: AAH83089.1.
RefSeqiNP_083404.2. NM_029128.2.
XP_006521731.1. XM_006521668.1.
XP_006521733.1. XM_006521670.2.
UniGeneiMm.275354.

3D structure databases

ProteinModelPortaliB8ZXI1.
SMRiB8ZXI1. Positions 119-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023387.

Proteomic databases

EPDiB8ZXI1.
MaxQBiB8ZXI1.
PaxDbiB8ZXI1.
PeptideAtlasiB8ZXI1.
PRIDEiB8ZXI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi106248.
KEGGimmu:106248.
UCSCiuc007zgl.1. mouse. [B8ZXI1-1]

Organism-specific databases

CTDi106248.
MGIiMGI:1922194. Qtrtd1.

Phylogenomic databases

eggNOGiKOG3909. Eukaryota.
COG0343. LUCA.
InParanoidiB8ZXI1.
KOiK15407.
OMAiGCRLGKI.
PhylomeDBiB8ZXI1.
TreeFamiTF317105.

Enzyme and pathway databases

UniPathwayiUPA00392.

Miscellaneous databases

PROiB8ZXI1.
SOURCEiSearch...

Family and domain databases

Gene3Di3.20.20.105. 2 hits.
HAMAPiMF_03043. QTRD1. 1 hit.
InterProiIPR028592. QTRTD1.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 2 hits.
TIGRFAMsiTIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQTRD1_MOUSE
AccessioniPrimary (citable) accession number: B8ZXI1
Secondary accession number(s): A9C446, Q5XK35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: September 7, 2016
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.