B8ZUW8 (ADD_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	MLBr00700

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Adenosine deaminase HAMAP MF_00540		PRO_1000146573
Sites
	Active site	211	1	Proton donor By similarity
	Metal binding	19	1	Zinc; catalytic By similarity
	Metal binding	21	1	Zinc; catalytic By similarity
	Metal binding	208	1	Zinc; catalytic By similarity
	Metal binding	300	1	Zinc; catalytic By similarity
	Binding site	21	1	Substrate By similarity
	Binding site	23	1	Substrate By similarity
	Binding site	181	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Site	232	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8ZUW8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: ED0C43B5C92908F5	FASTA	362	39,637
	10 20 30 40 50 60 MNTPLHLENI KQAPKALLHD HLDGGLRPAT VLDIAGQVGY DRLPATDVES LETWFRTASH 70 80 90 100 110 120 SGSLERYLEP FSHTVAVMQT PEALHRVAYE CVEDLAADSV VYAEVRFAPE LHIDEGLSFD 130 140 150 160 170 180 EVLASVLAGF ADGERACAAE GNAITVRCLV TAMRHAAMSR EIAELAIRFR DKGVVGFDIA 190 200 210 220 230 240 GAEAGHPPTR HLDAFEYMRS NNARFTIHAG EAFGLPSIHE AIAFCGADRL GHGVRIVDDI 250 260 270 280 290 300 DVDPGGGIRL GPLASILRDK RIPLELCPSS NLQTGAVASI TEHPFDLLAW ARFRVTVNTD 310 320 330 340 350 360 NRLMSDTSMS LEMHRLVEAF GYGWGDLERF TINAMKSAFI PFDQRLAIID EVIKPRFAVL VG

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR70794.1.
RefSeq	YP_002503190.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZUW8.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZUW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000085305; EBMYCP00000082393; EBMYCG00000086062.
GeneID	7325971.
GenomeReviews	Gene locus MLBr00700 in contig FM211192_GR.
PATRIC	18042060. VBIMycLep121698_1261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016615.
HOGENOM	HBG630382.
OMA	PYYMAMN.
ProtClustDB	PRK09358.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_MYCLB

Accession

Primary (citable) accession number: B8ZUW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program