B8ZUL1 (PYRB_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	MLBr00532

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_1000116152

Sequences

	Sequence		Length	Mass (Da)
	B8ZUL1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: DDC76151F3EAEE86	FASTA	321	34,257
	10 20 30 40 50 60 MMRRHLLNAG DLSRDDAIAI FDDADRFAHA LVGREIKKLP TLRGRTVITM FYENSTRTRV 70 80 90 100 110 120 SFEVAGKWMS ANVINVSPAG SSVGKGESLR DTALTLRAAG ADALIIRHPA SGAAHLLADW 130 140 150 160 170 180 TAAETGEDGP AVINAGDGTH EHPTQALLDA LTIRQRLGSI EGRRILIVGD ILHSRVARSN 190 200 210 220 230 240 VVLLDTLGAE VVLVAPRTLL PIGVDGWPAT VSCDFDAELP AADAVLMLRV QAERMNGGFF 250 260 270 280 290 300 PSVREYSCLY GLTERRQALL PGHAVVLHPG PMLRGMEIAS SVADSSQSAV LQQVSNGVHV 310 320 RMAVLFHVLV GTQSAEEEGA A

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR70625.1.
RefSeq	YP_002503083.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZUL1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZUL1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000086001; EBMYCP00000081470; EBMYCG00000085085.
GeneID	7326274.
GenomeReviews	Gene locus MLBr00532 in contig FM211192_GR.
PATRIC	18041392. VBIMycLep121698_0927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016463.
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_MYCLB

Accession

Primary (citable) accession number: B8ZUL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program