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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811SubstrateUniRule annotation
Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
Metal bindingi229 – 2291ManganeseUniRule annotation
Binding sitei229 – 2291SubstrateUniRule annotation
Metal bindingi249 – 2491Manganese; via tele nitrogenUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Active sitei273 – 2731UniRule annotation
Metal bindingi296 – 2961ManganeseUniRule annotation
Binding sitei389 – 3891GTPUniRule annotation
Binding sitei420 – 4201GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776GTPUniRule annotation
Nucleotide bindingi515 – 5184GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-2681-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:MLBr02624
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_1000192345Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi561304.MLBr_02624.

Structurei

3D structure databases

ProteinModelPortaliB8ZTI5.
SMRiB8ZTI5. Positions 12-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8ZTI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATIPGLD TAPTNHQKLL SWLEEVAELT HPAQVVFADG SDEEWQQLTE
60 70 80 90 100
RLVAAGTFKK LNDEKHPNSY LALSDPSDVA RVESRTFICS EREIDAGPTN
110 120 130 140 150
NWMDPAEMRS TMTELYRGCM RGRTMWVVPF CMGPPGAEDP KLGVEITDSE
160 170 180 190 200
YVVASMKVMT RMGTSALEKI GDDGFFVKAL HSVGAPLQPG QQDMPWPCNE
210 220 230 240 250
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM
260 270 280 290 300
LILKLISPEN KAYYFAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM
310 320 330 340 350
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD
360 370 380 390 400
DNDVWWEGLE GEPAHLIDWK GNDWYAGKTE TPAAHSNSRY CTPMSQCPIL
410 420 430 440 450
APEWDDPRGV PISVILFGAR RKTTVPLVTQ ARNWQHGVFM GATMGSEQTA
460 470 480 490 500
AAEGKVGTVR RDPMAMLPFM GYHVGDYFQH WIDLGKHSDE SNLPKVFFVN
510 520 530 540 550
WFRRGEGGKF LWPGFGENSR VLKWIVDRIE HKAGGQDTLI GIVPTATDLD
560 570 580 590 600
LDGLDISSDD VAKALAVDPA EWRNELPLIE EWFEFVGDKL PSGMQDEFEA

LKQRLAKED
Length:609
Mass (Da):67,732
Last modified:March 3, 2009 - v1
Checksum:i8916B6733622590B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72724.1.
RefSeqiWP_010909000.1. NC_011896.1.
YP_002504311.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72724; CAR72724; MLBr02624.
KEGGimlb:MLBr_02624.
PATRICi18049711. VBIMycLep121698_5054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72724.1.
RefSeqiWP_010909000.1. NC_011896.1.
YP_002504311.1. NC_011896.1.

3D structure databases

ProteinModelPortaliB8ZTI5.
SMRiB8ZTI5. Positions 12-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561304.MLBr_02624.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR72724; CAR72724; MLBr02624.
KEGGimlb:MLBr_02624.
PATRICi18049711. VBIMycLep121698_5054.

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciMLEP561304:GJP6-2681-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiPCKG_MYCLB
AccessioniPrimary (citable) accession number: B8ZTI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: April 1, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.