B8ZTI4 (TRMB_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	MLBr02622

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_1000149659
Regions
	Region	208 – 211	4	Substrate binding Potential
Sites
	Binding site	61	1	S-adenosyl-L-methionine By similarity
	Binding site	86	1	S-adenosyl-L-methionine By similarity
	Binding site	113	1	S-adenosyl-L-methionine By similarity
	Binding site	136	1	S-adenosyl-L-methionine By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	172	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	B8ZTI4 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 082DD06A64624D7B	FASTA	230	25,821
	10 20 30 40 50 60 MARHLPATAF RKRRSALSHA QRQTWERLWP EIGISAVSQT RSAERLDTGA WFGRSTLVVL 70 80 90 100 110 120 EVGCGSGTAT LAMAQHEPDI DVIAVEVYRR GLAQLLCAID RDQVHNIRMI HGNALYVLQY 130 140 150 160 170 180 LIAPRSLTGV RVFFPDPWPK VRHHKRRFLQ PATVELIADR LLPGGVLHTA TDHPDYAKQI 190 200 210 220 230 AKFGDGEPLL SRADGRTQLP ISTVRPTTKY ETKAQHAGNV VTELIWKKRS

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR72722.1.
RefSeq	YP_002504310.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZTI4.
ModBase	Search...
Protein-protein interaction databases
STRING	561304.MLBr_02622.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR72722; CAR72722; MLBr02622.
GeneID	7325534.
KEGG	mlb:MLBr_02622.
PATRIC	18049697. VBIMycLep121698_5047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	KFEDKAH.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	MLEP561304:GJP6-2679-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_MYCLB

Accession

Primary (citable) accession number: B8ZTI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program