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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611S-adenosyl-L-methionineUniRule annotation
Binding sitei86 – 861S-adenosyl-L-methionineUniRule annotation
Binding sitei113 – 1131S-adenosyl-L-methionineUniRule annotation
Active sitei136 – 1361By similarity
Binding sitei136 – 1361S-adenosyl-L-methionineUniRule annotation
Binding sitei140 – 1401SubstrateUniRule annotation
Binding sitei172 – 1721SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-2679-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MLBr02622
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230tRNA (guanine-N(7)-)-methyltransferasePRO_1000149659Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi561304.MLBr_02622.

Structurei

3D structure databases

ProteinModelPortaliB8ZTI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni208 – 2114Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B8ZTI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARHLPATAF RKRRSALSHA QRQTWERLWP EIGISAVSQT RSAERLDTGA
60 70 80 90 100
WFGRSTLVVL EVGCGSGTAT LAMAQHEPDI DVIAVEVYRR GLAQLLCAID
110 120 130 140 150
RDQVHNIRMI HGNALYVLQY LIAPRSLTGV RVFFPDPWPK VRHHKRRFLQ
160 170 180 190 200
PATVELIADR LLPGGVLHTA TDHPDYAKQI AKFGDGEPLL SRADGRTQLP
210 220 230
ISTVRPTTKY ETKAQHAGNV VTELIWKKRS
Length:230
Mass (Da):25,821
Last modified:March 3, 2009 - v1
Checksum:i082DD06A64624D7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72722.1.
RefSeqiWP_010908999.1. NC_011896.1.
YP_002504310.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72722; CAR72722; MLBr02622.
GeneIDi7325534.
KEGGimlb:MLBr_02622.
PATRICi18049697. VBIMycLep121698_5047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72722.1.
RefSeqiWP_010908999.1. NC_011896.1.
YP_002504310.1. NC_011896.1.

3D structure databases

ProteinModelPortaliB8ZTI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561304.MLBr_02622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR72722; CAR72722; MLBr02622.
GeneIDi7325534.
KEGGimlb:MLBr_02622.
PATRICi18049697. VBIMycLep121698_5047.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciMLEP561304:GJP6-2679-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiTRMB_MYCLB
AccessioniPrimary (citable) accession number: B8ZTI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: February 4, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.