Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei51D-inositol 3-phosphateUniRule annotation1
Binding sitei19UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei741D-inositol 3-phosphateUniRule annotation1
Binding sitei1071D-inositol 3-phosphateUniRule annotation1
Binding sitei1311D-inositol 3-phosphateUniRule annotation1
Binding sitei1511D-inositol 3-phosphateUniRule annotation1
Binding sitei225UDP-GlcNAcUniRule annotation1
Binding sitei230UDP-GlcNAcUniRule annotation1
Binding sitei283UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi292Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi293Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi295Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei305UDP-GlcNAcUniRule annotation1
Binding sitei313UDP-GlcNAcUniRule annotation1
Metal bindingi319MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MLBr02443
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006900 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001351 – 428D-inositol 3-phosphate glycosyltransferaseAdd BLAST428

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8ZT88.
SMRiB8ZT88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 12UDP-GlcNAc bindingUniRule annotation2
Regioni16 – 211D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZT88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVHTSPLA QPGIGDAGGM NVYVLQSALH LARRGIEVEI FTRATASADP
60 70 80 90 100
PIVWVAPGVL VRNVVAGPFE GLDKYDLPTQ LCAFAAGVLR AEAAHEPGYY
110 120 130 140 150
DIVHSHYWLS GQVGWLARDR WAVPLVHTAH TLAAVKNAAL ADGDAAEPPL
160 170 180 190 200
RSVGEQQVVD EADRMIVNTD DEARQLISIH RADPAKIDVA HPGVDLDMFR
210 220 230 240 250
PGDRRAARAA LGLPLDGNVV AFVGRIQPLK APDIVLRAAA KLPQVRIVVA
260 270 280 290 300
GGPSGSGLAS PDGLVRLADE LGITARVTFL PPQSRTNLAT VFQAADLVAV
310 320 330 340 350
PSYSESFGLV AVEAQACGTP VVAAAVGGLP VAVRDGVTGT LVFGHNVGHW
360 370 380 390 400
ADAVDQLLRL SAGPQARAIS RAAVVHAAQF SWDNTTDALL ASYRRAIGDF
410 420
TATRQHRVRD LVATRKPRRW ISRRGMGA
Length:428
Mass (Da):45,292
Last modified:March 3, 2009 - v1
Checksum:iA14F9F0187E3587C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72542.1.
RefSeqiWP_010908903.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72542; CAR72542; MLBr02443.
KEGGimlb:MLBr02443.
PATRICi18049021. VBIMycLep121698_4710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72542.1.
RefSeqiWP_010908903.1. NC_011896.1.

3D structure databases

ProteinModelPortaliB8ZT88.
SMRiB8ZT88.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR72542; CAR72542; MLBr02443.
KEGGimlb:MLBr02443.
PATRICi18049021. VBIMycLep121698_4710.

Phylogenomic databases

KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCLB
AccessioniPrimary (citable) accession number: B8ZT88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.