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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei5 – 511D-inositol 3-phosphateUniRule annotation
Binding sitei19 – 191UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei74 – 7411D-inositol 3-phosphateUniRule annotation
Binding sitei107 – 10711D-inositol 3-phosphateUniRule annotation
Binding sitei131 – 13111D-inositol 3-phosphateUniRule annotation
Binding sitei151 – 15111D-inositol 3-phosphateUniRule annotation
Binding sitei225 – 2251UDP-GlcNAcUniRule annotation
Binding sitei230 – 2301UDP-GlcNAcUniRule annotation
Binding sitei283 – 2831UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi292 – 2921Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi293 – 2931Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi295 – 2951Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei305 – 3051UDP-GlcNAcUniRule annotation
Binding sitei313 – 3131UDP-GlcNAcUniRule annotation
Metal bindingi319 – 3191MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-2497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MLBr02443
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006900 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428D-inositol 3-phosphate glycosyltransferasePRO_0000400135Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB8ZT88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 122UDP-GlcNAc bindingUniRule annotation
Regioni16 – 2161D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZT88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVHTSPLA QPGIGDAGGM NVYVLQSALH LARRGIEVEI FTRATASADP
60 70 80 90 100
PIVWVAPGVL VRNVVAGPFE GLDKYDLPTQ LCAFAAGVLR AEAAHEPGYY
110 120 130 140 150
DIVHSHYWLS GQVGWLARDR WAVPLVHTAH TLAAVKNAAL ADGDAAEPPL
160 170 180 190 200
RSVGEQQVVD EADRMIVNTD DEARQLISIH RADPAKIDVA HPGVDLDMFR
210 220 230 240 250
PGDRRAARAA LGLPLDGNVV AFVGRIQPLK APDIVLRAAA KLPQVRIVVA
260 270 280 290 300
GGPSGSGLAS PDGLVRLADE LGITARVTFL PPQSRTNLAT VFQAADLVAV
310 320 330 340 350
PSYSESFGLV AVEAQACGTP VVAAAVGGLP VAVRDGVTGT LVFGHNVGHW
360 370 380 390 400
ADAVDQLLRL SAGPQARAIS RAAVVHAAQF SWDNTTDALL ASYRRAIGDF
410 420
TATRQHRVRD LVATRKPRRW ISRRGMGA
Length:428
Mass (Da):45,292
Last modified:March 3, 2009 - v1
Checksum:iA14F9F0187E3587C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72542.1.
RefSeqiWP_010908903.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72542; CAR72542; MLBr02443.
PATRICi18049021. VBIMycLep121698_4710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72542.1.
RefSeqiWP_010908903.1. NC_011896.1.

3D structure databases

ProteinModelPortaliB8ZT88.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR72542; CAR72542; MLBr02443.
PATRICi18049021. VBIMycLep121698_4710.

Phylogenomic databases

OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-2497-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiMSHA_MYCLB
AccessioniPrimary (citable) accession number: B8ZT88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 3, 2009
Last modified: May 11, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.