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B8ZSY2 (MEND_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:MLBr02270
OrganismMycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]
Taxonomic identifier561304 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5565562-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_1000187082

Sequences

Sequence LengthMass (Da)Tools
B8ZSY2 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 63646C2CCD41D1F8

FASTA55658,351
        10         20         30         40         50         60 
MNPSTTQARV VVDELIRGGV RDVVLCPGSR NAPLAFALQD ADHAGRIRLH VRIDERTAGY 

        70         80         90        100        110        120 
LAIGLAVAAG APVCVAMTSG TAVANLGPAV VEANYARVPL IVLSANRPYE LLGTGANQTM 

       130        140        150        160        170        180 
EQLGYFGTQV RAAISLGLAE DAPERLDSLN ATWRSATCRV LVAATGSRTA NAGPVQFDIP 

       190        200        210        220        230        240 
LREPLIPDPE PHGAITPQGR PGGKPWTYIP QVTFDQPLEI DLSADTVVIA GHGAGVHPNL 

       250        260        270        280        290        300 
TELPIVAEPT APFGPYRPYP LHPLALPLLH PKQVIMLGRP TLHRPVSALL ADPQVPVYAL 

       310        320        330        340        350        360 
TTGPCWPDVS GNSQAAGTRA VTTGTPHPAW LQRCAEMNQH AISTVRSQLA AHPLITGLHV 

       370        380        390        400        410        420 
AAAVADALRP GDQLVLGASN PVRDMALVGL STDGIQVRSN RGVAGIDGTV STAIGAALAY 

       430        440        450        460        470        480 
ERSPGYAGST DRPARTVALI GDLAFVHDSS GLLIGPTEPT PQQLKIVVSN DNGGGIFELL 

       490        500        510        520        530        540 
EQGDPRLSAV SSRIFGTPHD VDVGALCRAY HVEGRQIEVD KLREALDEPG VGMRVLEVKA 

       550 
DRSSLRQLHA AITATL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM211192 Genomic DNA. Translation: CAR72368.1.
RefSeqYP_002504108.1. NC_011896.1.

3D structure databases

ProteinModelPortalB8ZSY2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING561304.MLBr_02270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR72368; CAR72368; MLBr02270.
GeneID7324873.
KEGGmlb:MLBr_02270.
PATRIC18048313. VBIMycLep121698_4359.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
KOK02551.
OMASEIWNIT.
OrthoDBEOG6NWBQW.

Enzyme and pathway databases

BioCycMLEP561304:GJP6-2320-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

Gene3D3.40.50.970. 2 hits.
HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
SUPFAMSSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_MYCLB
AccessionPrimary (citable) accession number: B8ZSY2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways