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B8ZSY2

- MEND_MYCLB

UniProt

B8ZSY2 - MEND_MYCLB

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-2320-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:MLBr02270
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5565562-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_1000187082Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi561304.MLBr_02270.

Structurei

3D structure databases

ProteinModelPortaliB8ZSY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
KOiK02551.
OMAiSEIWNIT.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZSY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPSTTQARV VVDELIRGGV RDVVLCPGSR NAPLAFALQD ADHAGRIRLH
60 70 80 90 100
VRIDERTAGY LAIGLAVAAG APVCVAMTSG TAVANLGPAV VEANYARVPL
110 120 130 140 150
IVLSANRPYE LLGTGANQTM EQLGYFGTQV RAAISLGLAE DAPERLDSLN
160 170 180 190 200
ATWRSATCRV LVAATGSRTA NAGPVQFDIP LREPLIPDPE PHGAITPQGR
210 220 230 240 250
PGGKPWTYIP QVTFDQPLEI DLSADTVVIA GHGAGVHPNL TELPIVAEPT
260 270 280 290 300
APFGPYRPYP LHPLALPLLH PKQVIMLGRP TLHRPVSALL ADPQVPVYAL
310 320 330 340 350
TTGPCWPDVS GNSQAAGTRA VTTGTPHPAW LQRCAEMNQH AISTVRSQLA
360 370 380 390 400
AHPLITGLHV AAAVADALRP GDQLVLGASN PVRDMALVGL STDGIQVRSN
410 420 430 440 450
RGVAGIDGTV STAIGAALAY ERSPGYAGST DRPARTVALI GDLAFVHDSS
460 470 480 490 500
GLLIGPTEPT PQQLKIVVSN DNGGGIFELL EQGDPRLSAV SSRIFGTPHD
510 520 530 540 550
VDVGALCRAY HVEGRQIEVD KLREALDEPG VGMRVLEVKA DRSSLRQLHA

AITATL
Length:556
Mass (Da):58,351
Last modified:March 3, 2009 - v1
Checksum:i63646C2CCD41D1F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72368.1.
RefSeqiYP_002504108.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72368; CAR72368; MLBr02270.
GeneIDi7324873.
KEGGimlb:MLBr_02270.
PATRICi18048313. VBIMycLep121698_4359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72368.1 .
RefSeqi YP_002504108.1. NC_011896.1.

3D structure databases

ProteinModelPortali B8ZSY2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561304.MLBr_02270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR72368 ; CAR72368 ; MLBr02270 .
GeneIDi 7324873.
KEGGi mlb:MLBr_02270.
PATRICi 18048313. VBIMycLep121698_4359.

Phylogenomic databases

eggNOGi COG1165.
KOi K02551.
OMAi SEIWNIT.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci MLEP561304:GJP6-2320-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiMEND_MYCLB
AccessioniPrimary (citable) accession number: B8ZSY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3