B8ZSV1 (B8ZSV1_MYCLB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL CAR72303.1
Ordered Locus Names:	MLBr02206

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL CAR72303.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	58	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	299	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	445	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	501	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	504	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	B8ZSV1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: E0CF4D278302FDCE	FASTA	556	59,540
	10 20 30 40 50 60 MCLAVGVGVR APKHVPQIRR LGRAGRRLRC VTNCALGSCP IVTVQQPGRD FSSPREECGV 70 80 90 100 110 120 FGVWAPGELV AKLTYFGLYA LQHRGQEAAG IAVADGSQVL VFKDLGLVSQ VFDEQTLAAM 130 140 150 160 170 180 EGHVAIGHCR YSTTGDTTWE NAQPVFRNIA AGSGVALGHN GNLVNTAELA ARARDAGLIA 190 200 210 220 230 240 KRCPAPATTD SDILGALLAH GAADSTLEQA ALELLPTVRG AFCLTFMDEN TLYACRDPYG 250 260 270 280 290 300 VRPLSLGRLD RGWVVASETA GLDIVGASFV RDIEPGELLA IDADGVRSTR FANPTPKGCV 310 320 330 340 350 360 FEYVYLARPD STLAGRSVHG TRVEIGRRLA RECPVEADLV IGVPESGTPA AVGYAQESGI 370 380 390 400 410 420 SYGQGLMKNA YVGRTFIQPS QTIRQLGIRL KLNPLKEVIR GKRLIVVDDS VVRGNTQRAL 430 440 450 460 470 480 VRMLREAGAV ELHVRIASPP VKWPCFYGID FPSPAELIAN VVADEEEMLE AVRQGIGADT 490 500 510 520 530 540 LGYISLRGMI AASEQPASRL CYACFDGRYP IELPSEAMLG KNVIEHMLAN AARGAGLRDL 550 AADQVPVDAD ENCVWR

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR72303.1.
RefSeq	YP_002504077.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZSV1.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZSV1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000086353; EBMYCP00000081786; EBMYCG00000087135.
GeneID	7325041.
GenomeReviews	Gene locus MLBr02206 in contig FM211192_GR.
PATRIC	18047987. VBIMycLep121698_4197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016850.
HOGENOM	HBG392416.
OMA	VWAPGEE.
ProtClustDB	PRK07847.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8ZSV1_MYCLB

Accession

Primary (citable) accession number: B8ZSV1

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)