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B8ZSN3

- MASZ_MYCLB

UniProt

B8ZSN3 - MASZ_MYCLB

Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi439 – 4391Magnesium
    Binding sitei439 – 4391GlyoxylateUniRule annotation
    Metal bindingi467 – 4671Magnesium
    Binding sitei548 – 5481Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei638 – 6381Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMLEP561304:GJP6-2112-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:MLBr02069
    OrganismiMycobacterium leprae (strain Br4923)
    Taxonomic identifieri561304 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000006900: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 731731Malate synthase GPRO_1000147426Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi561304.MLBr_02069.

    Structurei

    Secondary structure

    1
    731
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi10 – 134
    Helixi14 – 2310
    Helixi32 – 7039
    Helixi78 – 8710
    Helixi107 – 1104
    Beta strandi116 – 1205
    Helixi124 – 1329
    Beta strandi135 – 1373
    Helixi138 – 1436
    Helixi162 – 17918
    Helixi187 – 1893
    Helixi214 – 2163
    Beta strandi217 – 2204
    Beta strandi232 – 2354
    Beta strandi238 – 2436
    Beta strandi260 – 2645
    Beta strandi267 – 2748
    Helixi282 – 29716
    Beta strandi302 – 3065
    Beta strandi309 – 3135
    Beta strandi319 – 3224
    Beta strandi328 – 3314
    Beta strandi336 – 3405
    Beta strandi347 – 3537
    Beta strandi359 – 3646
    Helixi365 – 37713
    Helixi378 – 3803
    Helixi385 – 3873
    Beta strandi394 – 3963
    Beta strandi398 – 4025
    Helixi408 – 42518
    Beta strandi432 – 4387
    Helixi441 – 4444
    Helixi447 – 4537
    Turni454 – 4574
    Beta strandi458 – 4636
    Helixi465 – 47511
    Helixi477 – 4793
    Turni485 – 4873
    Helixi488 – 4903
    Helixi492 – 50716
    Turni511 – 5133
    Beta strandi514 – 5185
    Beta strandi523 – 5253
    Helixi527 – 5337
    Helixi536 – 5394
    Beta strandi543 – 5497
    Helixi550 – 56213
    Helixi565 – 5728
    Helixi580 – 5834
    Helixi596 – 62025
    Beta strandi625 – 6284
    Beta strandi634 – 6374
    Helixi639 – 65416
    Helixi660 – 67718
    Turni678 – 6803
    Helixi691 – 6933
    Helixi695 – 70511
    Helixi707 – 7093
    Helixi711 – 7133
    Helixi716 – 72813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EX4X-ray2.10A/B1-731[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni464 – 4674Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    KOiK01638.
    OMAiSTIMDFE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8ZSN3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDRVSAGNL RVARVLYDFV NNEALPGTDI NPNSFWSGVA KVVADLTPQN    50
    QSLLNSRDEL QAQIDKWHRH RVIEPFDVDA YRQFLIDIGY LLPEPDDFTI 100
    STSGVDDEIT MTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDTIPE 150
    TEGAEKGSEY NKIRGDKVIA YARKFMDQAV PLASDSWTNA TGVSIFDGQL 200
    QIAIGTNSTG LASPEKFVGY NRQLRSSNWS VLLANHGLHI EVLIDPESPI 250
    GKTDPVGIKD VILESAITTI MDFEDSVTAV DADDKVRGYR NWLGLNKGDL 300
    TEEVNKDGKT FTRVLNADRS YTTPDGGELT LPGRSLLFVR NVGHLTTSDA 350
    ILVDGGDGQE KEVFEGIIDA VFTGLAAIHG LKTGEANGPL TNSRTGSIYI 400
    VKPKMHGPAE VAFTCELFSR VEDVLGLPQG TLKVGIMDEE RRTTLNLKAC 450
    IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN STWIKAYEDA 500
    NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP 550
    TAATLHAMHY HQVDVAAVQQ ELTGQRRATV DQLLTIPLAK ELAWAPEEIR 600
    EEVDNDCQSI LGYVVRWVDQ GIGCSKVPDI HNVALMEDRA TLRISSQLLA 650
    NWLRHGVITS EDVRASLERM APLVDQQNAE DPAYRPMAPN FDDSIAFLAA 700
    QELILSGAQQ PNGYTEPILH RRRREFKAQN R 731
    Length:731
    Mass (Da):80,143
    Last modified:March 3, 2009 - v1
    Checksum:i3878C0DAE26DECDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211192 Genomic DNA. Translation: CAR72166.1.
    RefSeqiYP_002504009.1. NC_011896.1.

    Genome annotation databases

    EnsemblBacteriaiCAR72166; CAR72166; MLBr02069.
    GeneIDi7325139.
    KEGGimlb:MLBr_02069.
    PATRICi18047419. VBIMycLep121698_3918.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211192 Genomic DNA. Translation: CAR72166.1 .
    RefSeqi YP_002504009.1. NC_011896.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EX4 X-ray 2.10 A/B 1-731 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561304.MLBr_02069.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR72166 ; CAR72166 ; MLBr02069 .
    GeneIDi 7325139.
    KEGGi mlb:MLBr_02069.
    PATRICi 18047419. VBIMycLep121698_3918.

    Phylogenomic databases

    eggNOGi COG2225.
    KOi K01638.
    OMAi STIMDFE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MLEP561304:GJP6-2112-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Br4923.
    2. "The structure of glcb from mycobacterium leprae."
      Seattle structural genomics center for infectious disease (SSGCID)
      Submitted (APR-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH MAGNESIUM ION, SUBUNIT.

    Entry informationi

    Entry nameiMASZ_MYCLB
    AccessioniPrimary (citable) accession number: B8ZSN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3