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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi439Magnesium1
Binding sitei439GlyoxylateUniRule annotation1
Metal bindingi467Magnesium1
Binding sitei548Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei638Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MLBr02069
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006900 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001474261 – 731Malate synthase GAdd BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei624Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1731
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi10 – 13Combined sources4
Helixi14 – 23Combined sources10
Helixi32 – 70Combined sources39
Helixi78 – 87Combined sources10
Helixi107 – 110Combined sources4
Beta strandi116 – 120Combined sources5
Helixi124 – 132Combined sources9
Beta strandi135 – 137Combined sources3
Helixi138 – 143Combined sources6
Helixi162 – 179Combined sources18
Helixi187 – 189Combined sources3
Beta strandi207 – 209Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 220Combined sources4
Beta strandi232 – 235Combined sources4
Beta strandi238 – 243Combined sources6
Helixi251 – 253Combined sources3
Beta strandi260 – 264Combined sources5
Beta strandi267 – 274Combined sources8
Helixi282 – 297Combined sources16
Beta strandi302 – 306Combined sources5
Beta strandi309 – 313Combined sources5
Beta strandi319 – 322Combined sources4
Beta strandi328 – 331Combined sources4
Beta strandi336 – 340Combined sources5
Beta strandi347 – 353Combined sources7
Beta strandi359 – 364Combined sources6
Helixi365 – 377Combined sources13
Helixi378 – 380Combined sources3
Helixi385 – 387Combined sources3
Beta strandi394 – 396Combined sources3
Beta strandi398 – 402Combined sources5
Helixi408 – 425Combined sources18
Beta strandi432 – 438Combined sources7
Helixi441 – 444Combined sources4
Helixi447 – 453Combined sources7
Turni454 – 457Combined sources4
Beta strandi458 – 463Combined sources6
Helixi465 – 475Combined sources11
Helixi477 – 479Combined sources3
Turni485 – 487Combined sources3
Helixi488 – 490Combined sources3
Helixi492 – 507Combined sources16
Turni511 – 513Combined sources3
Beta strandi514 – 518Combined sources5
Beta strandi523 – 525Combined sources3
Helixi527 – 533Combined sources7
Helixi536 – 539Combined sources4
Beta strandi543 – 549Combined sources7
Helixi550 – 562Combined sources13
Helixi565 – 572Combined sources8
Helixi580 – 583Combined sources4
Helixi596 – 620Combined sources25
Beta strandi625 – 628Combined sources4
Beta strandi634 – 637Combined sources4
Helixi639 – 654Combined sources16
Helixi660 – 677Combined sources18
Turni678 – 680Combined sources3
Helixi691 – 693Combined sources3
Helixi695 – 705Combined sources11
Helixi707 – 709Combined sources3
Helixi711 – 713Combined sources3
Helixi716 – 728Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EX4X-ray2.10A/B1-731[»]
SMRiB8ZSN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni464 – 467Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZSN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NNEALPGTDI NPNSFWSGVA KVVADLTPQN
60 70 80 90 100
QSLLNSRDEL QAQIDKWHRH RVIEPFDVDA YRQFLIDIGY LLPEPDDFTI
110 120 130 140 150
STSGVDDEIT MTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDTIPE
160 170 180 190 200
TEGAEKGSEY NKIRGDKVIA YARKFMDQAV PLASDSWTNA TGVSIFDGQL
210 220 230 240 250
QIAIGTNSTG LASPEKFVGY NRQLRSSNWS VLLANHGLHI EVLIDPESPI
260 270 280 290 300
GKTDPVGIKD VILESAITTI MDFEDSVTAV DADDKVRGYR NWLGLNKGDL
310 320 330 340 350
TEEVNKDGKT FTRVLNADRS YTTPDGGELT LPGRSLLFVR NVGHLTTSDA
360 370 380 390 400
ILVDGGDGQE KEVFEGIIDA VFTGLAAIHG LKTGEANGPL TNSRTGSIYI
410 420 430 440 450
VKPKMHGPAE VAFTCELFSR VEDVLGLPQG TLKVGIMDEE RRTTLNLKAC
460 470 480 490 500
IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN STWIKAYEDA
510 520 530 540 550
NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP
560 570 580 590 600
TAATLHAMHY HQVDVAAVQQ ELTGQRRATV DQLLTIPLAK ELAWAPEEIR
610 620 630 640 650
EEVDNDCQSI LGYVVRWVDQ GIGCSKVPDI HNVALMEDRA TLRISSQLLA
660 670 680 690 700
NWLRHGVITS EDVRASLERM APLVDQQNAE DPAYRPMAPN FDDSIAFLAA
710 720 730
QELILSGAQQ PNGYTEPILH RRRREFKAQN R
Length:731
Mass (Da):80,143
Last modified:March 3, 2009 - v1
Checksum:i3878C0DAE26DECDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72166.1.
RefSeqiWP_012634443.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR72166; CAR72166; MLBr02069.
KEGGimlb:MLBr02069.
PATRICi18047419. VBIMycLep121698_3918.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR72166.1.
RefSeqiWP_012634443.1. NC_011896.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EX4X-ray2.10A/B1-731[»]
SMRiB8ZSN3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR72166; CAR72166; MLBr02069.
KEGGimlb:MLBr02069.
PATRICi18047419. VBIMycLep121698_3918.

Phylogenomic databases

KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_MYCLB
AccessioniPrimary (citable) accession number: B8ZSN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.