B8ZSC1 (EFTU_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf
Ordered Locus Names:	MLBr01877

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118		PRO_1000201408
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	83 – 87	5	GTP By similarity
	Nucleotide binding	138 – 141	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8ZSC1 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D9CB88343C642778	FASTA	396	43,668
	10 20 30 40 50 60 MAKAKFERTK PHVNIGTIGH VDHGKTTLTA AITKVLHDKF PNLNESRAFD QIDNAPEERQ 70 80 90 100 110 120 RGITINISHV EYQTEKRHYA HVDAPGHADY IKNMITGAAQ MDGAILVVAA TDGPMPQTRE 130 140 150 160 170 180 HVLLARQVGV PYILVALNKS DAVDDEELLE LVEMEVRELL AAQEFDEDAP VVRVSALKAL 190 200 210 220 230 240 EGDAKWVESV TQLMDAVDES IPAPVRETDK PFLMPVEDVF TITGRGTVVT GRVERGVVNV 250 260 270 280 290 300 NEEVEIVGIR QTTTKTTVTG VEMFRKLLDQ GQAGDNVGLL LRGIKREDVE RGQVVIKPGT 310 320 330 340 350 360 TTPHTEFEGQ VYILSKDEGG RHTPFFNNYR PQFYFRTTDV TGVVTLPEGT EMVMPGDNTN 370 380 390 ISVTLIQPVA MDEGLRFAIR EGGRTVGAGR VVKIIK

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71973.1.
RefSeq	YP_002503897.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZSC1.
SMR	B8ZSC1. Positions 2-395.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR71973; CAR71973; MLBr01877.
GeneID	7325921.
KEGG	mlb:MLBr_01877.
PATRIC	18046718. VBIMycLep121698_3571.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
KO	K02358.
OMA	CEFVGYN.
ProtClustDB	PRK00049.
Enzyme and pathway databases
BioCyc	MLEP561304:GJP6-1916-MONOMER.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_MYCLB

Accession

Primary (citable) accession number: B8ZSC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program