DNA ligase - Mycobacterium leprae (strain Br4923)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.2

Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	MLBr01705

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	694 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588
Catalytic activity	NAD⁺ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity. HAMAP MF_01588
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 694

694

DNA ligase HAMAP MF_01588

PRO_0000380427

Regions

Domain

607 – 694

88

BRCT

Nucleotide binding

41 – 45

5

NAD By similarity

Nucleotide binding

91 – 92

2

NAD By similarity

Sites

Active site

123

1

N6-AMP-lysine intermediate By similarity

Metal binding

418

1

Zinc By similarity

Metal binding

421

1

Zinc By similarity

Metal binding

437

1

Zinc By similarity

Metal binding

443

1

Zinc By similarity

Binding site

121

1

NAD By similarity

Binding site

144

1

NAD By similarity

Binding site

184

1

NAD By similarity

Binding site

300

1

NAD By similarity

Binding site

324

1

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

B8ZS27 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: CFE712B6AFDAA9D5
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FASTA

694

75,912

        10         20         30         40         50         60 
MSSPEADPIG PEVLQQWRKL TEEVREHQFR YYVRDAPIIS DAEFDALLDR LTVLEEQHPE 

        70         80         90        100        110        120 
LCTPDSPTQL VGGAGFMTDF VATEHLERMF SLDNAFSGDE LNAWVTRIRA EIGNDAHYLC 

       130        140        150        160        170        180 
ELKIDGVALS LVYRDGRLTR ATTRGDGRTG EDVTLNARTI EDVPERLTVS DIYPLPEVLE 

       190        200        210        220        230        240 
VRGEVFFRVV DFQALNTSLV EEGKNPFANP RNSAAGSLRQ KDPAVTAHRK LRMICHGLGH 

       250        260        270        280        290        300 
IEGFRPATQH QAYLALRDWG LPVSEHTTLV NDIAGVQGCI AYWAEHRHEV DHEIDGIVVK 

       310        320        330        340        350        360 
IDEVTLQRRL GSTSRAPRWA IAYKYLPEEA QTKLLDIRVN VGRTGRVTPF AFMTPVKVAG 

       370        380        390        400        410        420 
STVGQATLHN PSEVKRKGVL IGDTVVIRKA GDVIPEVLGP VVDLRDGSER EFVMPTTCPE 

       430        440        450        460        470        480 
CGTTLAPEKE GDADIRCPNA RSCPGQLRER VFHVASRSAL DIQGLGYEAG VALLAAQVIT 

       490        500        510        520        530        540 
SEGDLFTLTE KALLRTELFR NKAGELSANG KRLLVNVDKA KTAPLWRVLV ALSIRHVGPT 

       550        560        570        580        590        600 
AARALATEFG SVDAILAASP EQLAAVEGVG TTIAAAVTEW FTVDWHRVIV NKWRAAGVRM 

       610        620        630        640        650        660 
VDERDTSVLP TCEGLTIVVT GSLAGFSRDD AKEAIVARGG KVAGSVSKKI AYVVVGDLPG 

       670        680        690 
YKYDKAVELG VPILNEDGFR LLLEQGPPVQ QVVD

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References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae."
Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L.

Cole S.T.
Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71800.1.
RefSeq	YP_002503804.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZS27.
SMR	B8ZS27. Positions 10-328.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZS27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000085279; EBMYCP00000082787; EBMYCG00000086826.
GeneID	7326798.
GenomeReviews	Gene locus MLBr01705 in contig FM211192_GR.
PATRIC	18046020. VBIMycLep121698_3224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016652.
HOGENOM	HBG620317.
OMA	ENVRTIR.
ProtClustDB	PRK07956.
Family and domain databases
HAMAP	MF_01588. DNA_ligase_A. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR010994. RuvA_2-like. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 3 hits. SM00532. LIGANc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit.
TIGRFAMs	TIGR00575. Dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_MYCLB

Accession

Primary (citable) accession number: B8ZS27

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents