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B8ZS13 (B8ZS13_MYCLB) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022
Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022
Gene names
Name:gltS EMBL CAR71783.1
Synonyms:gltX HAMAP MF_00022
Ordered Locus Names:MLBr01688
OrganismMycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]
Taxonomic identifier561304 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022

Subunit structure

Monomer By similarity. HAMAP MF_00022

Subcellular location

Cytoplasm By similarity HAMAP MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
   Biological processProtein biosynthesis HAMAP MF_00022
   Cellular componentCytoplasm HAMAP MF_00022
   LigandATP-binding HAMAP MF_00022
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase HAMAP MF_00022
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif21 – 3111"HIGH" region By similarity HAMAP MF_00022
Motif265 – 2695"KMSKS" region By similarity HAMAP MF_00022

Sites

Binding site2681ATP By similarity HAMAP MF_00022

Sequences

Sequence LengthMass (Da)Tools
B8ZS13 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: F618C748C9F24A60

FASTA50255,305
        10         20         30         40         50         60 
MTAVTSDGTP QAAKVRVRFC PSPTGVPHVG MVRTALFNWA YARHTGGTFV LRIEDTDADR 

        70         80         90        100        110        120 
DSEESYLALL DALRWLGLNW DEGPEVGGPY GPYRQSQRTD IYREVVAKLL ATGEAYYAFS 

       130        140        150        160        170        180 
TPEEVENRHL AAGRNPKLGY DNFDRDLTDA QFSAYLAEGR KPVVRLRMPD EDISWDDLVR 

       190        200        210        220        230        240 
GTTTFAVGTV PDYVLTRASG DPLYTLVNPC DDALMKITHV LRGEDLLSST PRQVALYQAL 

       250        260        270        280        290        300 
IRIGMAERIP EFGHFPSVLG EGTKKLSKRE PQSNLFAHRD RGFIPEGLLN YLALLGWAIA 

       310        320        330        340        350        360 
DDHDLFSLDE MVAAFDVVDV NSNPARFDQK KADAVNAEHI RMLDSEDFAG RLRDYFTTHG 

       370        380        390        400        410        420 
YHIALDPANY EAGFVAAAQL VQTRIVVLGD AWDLLKFLND DEYSIDSKAA AKELDADAGP 

       430        440        450        460        470        480 
VLDVACAVLD SLVDWTTASI EDVLKVALIE GLGLKPRKVF GPIRVAATGA LVSPPLFESL 

       490        500 
ELLGRARSLQ RLSAARARVT SA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM211192 Genomic DNA. Translation: CAR71783.1.
RefSeqYP_002503790.1. NC_011896.1.

3D structure databases

ProteinModelPortalB8ZS13.
ModBaseSearch...

Protein-protein interaction databases

STRINGB8ZS13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000086147; EBMYCP00000082818; EBMYCG00000086772.
GeneID7325359.
GenomeReviewsGene locus MLBr01688 in contig FM211192_GR.
PATRIC18045960. VBIMycLep121698_3194.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016746.
HOGENOMHBG628189.
OMAMAHIPLI.
ProtClustDBPRK01406.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMsTIGR00464. GltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB8ZS13_MYCLB
AccessionPrimary (citable) accession number: B8ZS13
Entry history
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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