B8ZRW6 (B8ZRW6_MYCLB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605 EMBL CAR71710.1
Ordered Locus Names:	MLBr01615

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605 S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464
Subunit structure	Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464
Subcellular location	Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605 RuleBase RU003464
Cellular component	Cytoplasm HAMAP MF_00605 RuleBase RU003464
Ligand	S-adenosyl-L-methionine HAMAP MF_00605 RuleBase RU003464
Molecular function	Methyltransferase HAMAP MF_00605 RuleBase RU003464 EMBL CAR71710.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	B8ZRW6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 784410B2BE7E320B	FASTA	238	26,229
	10 20 30 40 50 60 MKIDIVTIFP AYLDPLRQSL PGKAIESGLV DVQVHDLRRW THDVHHSVDD VPYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEICFDETLL VIPTPAGALF TQATAQCWST ERHLVFACGR YEGIDQRVVD 130 140 150 160 170 180 DAVRRMRVEE VSIGDYVLPG GESAAVVMIE AVLRLVAGVL GNPASHRDDS HSPDLGRLLE 190 200 210 220 230 GPSYTRPPTW RGLDVPPVLL SGDHARIAAW RREASLRRTR KRRPDLSGAR FVWGLGLS

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71710.1.
RefSeq	YP_002503743.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZRW6.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZRW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000085729; EBMYCP00000081794; EBMYCG00000086452.
GeneID	7325412.
GenomeReviews	Gene locus MLBr01615 in contig FM211192_GR.
PATRIC	18045666. VBIMycLep121698_3050.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016420.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B8ZRW6_MYCLB

Accession

Primary (citable) accession number: B8ZRW6

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)