B8ZRW3 (RNH2_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	MLBr01611

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Ribonuclease HII HAMAP MF_00052_B		PRO_1000117679
Sites
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	40	1	Divalent metal cation By similarity
	Metal binding	131	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8ZRW3 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 3789259F169454C2	FASTA	240	25,600
	10 20 30 40 50 60 MATTWPPCRI IRKSGGLRGM WTLEYELQRS GLGPVAGVDE VGRGACAGPL VVAACVLGPG 70 80 90 100 110 120 RLEESLDDSK KLSAKGREML FPLICRYALA YHVVFIPSVE VDRHGVQVAN IEGMRRAVAG 130 140 150 160 170 180 LSVRPGYVLS DGFRVPGLSV PSLPVVGGDA VVACIAAASV LAKVSRDRLM VAMDADYPGY 190 200 210 220 230 240 GFAAHKGYCT RAHSLALTQL GPCPEHRYSF INVRRIVTRS NTRAVAGFTP APPAEHGECR

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71706.1.
RefSeq	YP_002503740.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZRW3.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZRW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000085547; EBMYCP00000082282; EBMYCG00000087053.
GeneID	7325773.
GenomeReviews	Gene locus MLBr01611 in contig FM211192_GR.
PATRIC	18045656. VBIMycLep121698_3045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCLB

Accession

Primary (citable) accession number: B8ZRW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program