B8ZRP4 (B8ZRP4_MYCLB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 2 HAMAP MF_00444

Gene names

Name:	clpP EMBL CAR71574.1
Synonyms:	clpP2 HAMAP MF_00444
Ordered Locus Names:	MLBr01480

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. RuleBase RU003567 HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	122	1	By similarity HAMAP MF_00444
	Active site	147	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B8ZRP4 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 71B48E1AEF230AF1	FASTA	224	24,582
	10 20 30 40 50 60 MIAKQRARFV SVGAYRTPES RYPTVGHVTD IRSNSQGFNL TDSVYERLLS ERIIFLGSEV 70 80 90 100 110 120 NDNIANRLCA QILLLAAEDA FKDISLYINS PGGSISAGMA IYDTMVLAPC DIATYAMGMA 130 140 150 160 170 180 ASMGEFLLAA GTRGKRYALP HARILMHQPL GGVTGSAADI AIQAEQFAVI KKEMFRLNAE 190 200 210 220 FTGQPIERIE ADSDRDRWFT ASEALEYGFV DHIITRAHVN GEVQ

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71574.1.
RefSeq	YP_002503671.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZRP4.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZRP4.
Protein family/group databases
MEROPS	S14.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000085299; EBMYCP00000081981; EBMYCG00000087001.
GeneID	7327276.
GenomeReviews	Gene locus MLBr01480 in contig FM211192_GR.
PATRIC	18045120. VBIMycLep121698_2778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015874.
HOGENOM	HBG558421.
OMA	NAICAQL.
ProtClustDB	PRK00277.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B8ZRP4_MYCLB

Accession

Primary (citable) accession number: B8ZRP4

Entry history

Integrated into UniProtKB/TrEMBL:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)