B8ZRH2 (PPNK_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	MLBr01359

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_1000133577

Sequences

	Sequence		Length	Mass (Da)
	B8ZRH2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 0AD22150CC82D33B	FASTA	311	33,218
	10 20 30 40 50 60 MTPRKAAQRT VLLVVHTGRD EATETARRVK KIVGDNGIAL RVLSAEAVDR GSLHLALDNM 70 80 90 100 110 120 RAMGVDIEVV DADPHVAQGC ELVLVLGGDG TFLRAAELAR TARIPVLGVN LGRIGFLAEA 130 140 150 160 170 180 EAEAIDVVLE HVIARSYRVE ERLTLDIVVR QGGNIIDQGW ALNEASLEKG PRLGVLGVVV 190 200 210 220 230 240 EIEGRPVSTF GCDGVLVSTP TGSTAYAFSA GGPVLWPDLE AILVVPNNAH ALFGRPMVTS 250 260 270 280 290 300 PDATVAIELE ANGNDALVFC DGRREMIIPA GGRLEVTRCA TPVKWARLDS APFTDRLVSK 310 FRLPVTGWRG K

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed: 19881526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71454.1.
RefSeq	YP_002503599.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZRH2.
SMR	B8ZRH2. Positions 7-309.
ModBase	Search...
Protein-protein interaction databases
STRING	B8ZRH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000087285; EBMYCP00000082110; EBMYCG00000087484.
GeneID	7326027.
GenomeReviews	Gene locus MLBr01359 in contig FM211192_GR.
PATRIC	18044604. VBIMycLep121698_2520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016530.
HOGENOM	HBG713904.
OMA	GPVVWPE.
ProtClustDB	PRK03372.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_MYCLB

Accession

Primary (citable) accession number: B8ZRH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program