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B8ZR86

- BIOB_MYCLB

UniProt

B8ZR86 - BIOB_MYCLB

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Protein

Biotin synthase

Gene
bioB, MLBr01220
Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi157 – 1571Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi216 – 2161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-1242-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MLBr01220
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotationPRO_0000381479Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi561304.MLBr_01220.

Structurei

3D structure databases

ProteinModelPortaliB8ZR86.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiETHHNID.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZR86-1 [UniParc]FASTAAdd to Basket

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MTQAGTRSTN GDTENTDILA QARQQVLERG EGLSRDQVLQ MLWLPDDRLE    50
ELLALAHDVR MRWCGPEVEI EGIISLKTGG CPEDCHFCSQ SGLFMSPVRS 100
AWLDIRSLVE AAKQTAKSGA TEFCIVAAVR GPDERLMSQV AAGIEAIRNE 150
VEINIACSLG MLTVEQVEQL SGIGVHRYNH NLETARSFFT NVVTTHTWEE 200
RWQTLSMVRD AGMEVCCGGI LGMGETVEQR AEFALELAEL GPDEVPLNFL 250
NPRPGTPFGA LEVMPPSEAL KSVAAFRLAL PRTILRFAGG REITLGDLGA 300
KQGMLGGINA VIVGNYLTTL GRPAEADLRL LDDLQMPIKA LNASL 345
Length:345
Mass (Da):37,558
Last modified:March 3, 2009 - v1
Checksum:iC52DC8079BBD0191
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR71315.1.
RefSeqiWP_010908204.1. NC_011896.1.
YP_002503513.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR71315; CAR71315; MLBr01220.
GeneIDi7327079.
KEGGimlb:MLBr_01220.
PATRICi18044050. VBIMycLep121698_2250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR71315.1 .
RefSeqi WP_010908204.1. NC_011896.1.
YP_002503513.1. NC_011896.1.

3D structure databases

ProteinModelPortali B8ZR86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561304.MLBr_01220.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR71315 ; CAR71315 ; MLBr01220 .
GeneIDi 7327079.
KEGGi mlb:MLBr_01220.
PATRICi 18044050. VBIMycLep121698_2250.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi ETHHNID.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MLEP561304:GJP6-1242-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiBIOB_MYCLB
AccessioniPrimary (citable) accession number: B8ZR86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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