B8ZR86 (BIOB_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	MLBr01220

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381479
Sites
	Metal binding	81	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	85	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	88	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	124	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	157	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	216	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	286	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8ZR86 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: C52DC8079BBD0191	FASTA	345	37,558
	10 20 30 40 50 60 MTQAGTRSTN GDTENTDILA QARQQVLERG EGLSRDQVLQ MLWLPDDRLE ELLALAHDVR 70 80 90 100 110 120 MRWCGPEVEI EGIISLKTGG CPEDCHFCSQ SGLFMSPVRS AWLDIRSLVE AAKQTAKSGA 130 140 150 160 170 180 TEFCIVAAVR GPDERLMSQV AAGIEAIRNE VEINIACSLG MLTVEQVEQL SGIGVHRYNH 190 200 210 220 230 240 NLETARSFFT NVVTTHTWEE RWQTLSMVRD AGMEVCCGGI LGMGETVEQR AEFALELAEL 250 260 270 280 290 300 GPDEVPLNFL NPRPGTPFGA LEVMPPSEAL KSVAAFRLAL PRTILRFAGG REITLGDLGA 310 320 330 340 KQGMLGGINA VIVGNYLTTL GRPAEADLRL LDDLQMPIKA LNASL

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR71315.1.
RefSeq	YP_002503513.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZR86.
ModBase	Search...
Protein-protein interaction databases
STRING	561304.MLBr_01220.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR71315; CAR71315; MLBr01220.
GeneID	7327079.
KEGG	mlb:MLBr_01220.
PATRIC	18044050. VBIMycLep121698_2250.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	PRK06256.
Enzyme and pathway databases
BioCyc	MLEP561304:GJP6-1242-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_MYCLB

Accession

Primary (citable) accession number: B8ZR86

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program