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B8ZR86

- BIOB_MYCLB

UniProt

B8ZR86 - BIOB_MYCLB

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Protein

Biotin synthase

Gene

bioB

Organism
Mycobacterium leprae (strain Br4923)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi88 – 881Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi157 – 1571Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi216 – 2161Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-1242-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:MLBr01220
OrganismiMycobacterium leprae (strain Br4923)
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthasePRO_0000381479Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi561304.MLBr_01220.

Structurei

3D structure databases

ProteinModelPortaliB8ZR86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiETHHNID.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZR86-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQAGTRSTN GDTENTDILA QARQQVLERG EGLSRDQVLQ MLWLPDDRLE
60 70 80 90 100
ELLALAHDVR MRWCGPEVEI EGIISLKTGG CPEDCHFCSQ SGLFMSPVRS
110 120 130 140 150
AWLDIRSLVE AAKQTAKSGA TEFCIVAAVR GPDERLMSQV AAGIEAIRNE
160 170 180 190 200
VEINIACSLG MLTVEQVEQL SGIGVHRYNH NLETARSFFT NVVTTHTWEE
210 220 230 240 250
RWQTLSMVRD AGMEVCCGGI LGMGETVEQR AEFALELAEL GPDEVPLNFL
260 270 280 290 300
NPRPGTPFGA LEVMPPSEAL KSVAAFRLAL PRTILRFAGG REITLGDLGA
310 320 330 340
KQGMLGGINA VIVGNYLTTL GRPAEADLRL LDDLQMPIKA LNASL
Length:345
Mass (Da):37,558
Last modified:March 3, 2009 - v1
Checksum:iC52DC8079BBD0191
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR71315.1.
RefSeqiYP_002503513.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR71315; CAR71315; MLBr01220.
GeneIDi7327079.
KEGGimlb:MLBr_01220.
PATRICi18044050. VBIMycLep121698_2250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR71315.1 .
RefSeqi YP_002503513.1. NC_011896.1.

3D structure databases

ProteinModelPortali B8ZR86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561304.MLBr_01220.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR71315 ; CAR71315 ; MLBr01220 .
GeneIDi 7327079.
KEGGi mlb:MLBr_01220.
PATRICi 18044050. VBIMycLep121698_2250.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi ETHHNID.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MLEP561304:GJP6-1242-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923.

Entry informationi

Entry nameiBIOB_MYCLB
AccessioniPrimary (citable) accession number: B8ZR86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3