B8ZQK6 (GCST_MYCLB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	MLBr00865

Organism

Mycobacterium leprae (strain Br4923) [Complete proteome] [HAMAP]

Taxonomic identifier

561304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP-Rule MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP-Rule MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Aminomethyltransferase HAMAP-Rule MF_00259		PRO_1000125643

Sequences

	Sequence		Length	Mass (Da)
	B8ZQK6 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: B0B40CEFF6A6A094	FASTA	367	38,879
	10 20 30 40 50 60 MTDAPELLKG PLEDRHRELG ANFAEFGGWL MPVSYAGTVS EHSATRNAVG LFDVSHLGKA 70 80 90 100 110 120 LVRGPGAAQF VNSVLTNDLG RIRPGKAQYT LCCSESGGVI DDLIAYYVDD DEIFLVSNAA 130 140 150 160 170 180 NTAAVVDALQ AVVPAGLTII NQHRSHAVLA VQGPRSTDVL GELGLPTGID YMGYVDASYA 190 200 210 220 230 240 GVPVRVCRTG YTGEQGYELL PPWESADVVF DALVAAVVDA RGEPAGLGAR DTLRTEMGYP 250 260 270 280 290 300 LYGHELSLDI SPLQARCGWA IGWKKDAFLG RDALLAEKAA GPRRLLRGLR MAGRGVLRPG 310 320 330 340 350 360 LTVCAGDIPI GVTTSGTFSP TLQVGVALAL IDSEAAVQDG QQIIVDVRGR AVECEVVRPP FIEVKTR

References

[1]

"Comparative genomic and phylogeographic analysis of Mycobacterium leprae." Monot M., Honore N., Garnier T., Zidane N., Sherafi D., Paniz-Mondolfi A., Matsuoka M., Taylor G.M., Donoghue H.D., Bouwman A., Mays S., Watson C., Lockwood D., Khamispour A., Dowlati Y., Jianping S., Rea T.H., Vera-Cabrera L. , Stefani M.M., Banu S., Macdonald M., Sapkota B.R., Spencer J.S., Thomas J., Harshman K., Singh P., Busso P., Gattiker A., Rougemont J., Brennan P.J., Cole S.T. Nat. Genet. 41:1282-1289(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Br4923.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211192 Genomic DNA. Translation: CAR70960.1.
RefSeq	YP_002503283.1. NC_011896.1.
3D structure databases
ProteinModelPortal	B8ZQK6.
ModBase	Search...
Protein-protein interaction databases
STRING	561304.MLBr_00865.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR70960; CAR70960; MLBr00865.
GeneID	7327372.
KEGG	mlb:MLBr_00865.
PATRIC	18042730. VBIMycLep121698_1595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
KO	K00605.
OMA	KPAFWGR.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	MLEP561304:GJP6-882-MONOMER.
Family and domain databases
Gene3D	3.30.1360.120. 2 hits.
HAMAP	MF_00259. GcvT.
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. IPR027266. TrmE/GcvT_dom1. [Graphical view]
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_MYCLB

Accession

Primary (citable) accession number: B8ZQK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program