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Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium leprae (strain Br4923)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K(+)UniRule annotationNote: Monovalent cation. The ion could be potassium.UniRule annotation
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591GTPUniRule annotation
Binding sitei64 – 641GTPUniRule annotation
Metal bindingi118 – 1181Divalent metal cation 1UniRule annotation
Binding sitei121 – 1211GTPUniRule annotation
Metal bindingi159 – 1591Divalent metal cation 1UniRule annotation
Metal bindingi160 – 1601Divalent metal cation 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 324GTPUniRule annotation

GO - Molecular functioni

  1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
  2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
  3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
  2. F420-0 metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-773-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligaseUniRule annotation
Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
Gene namesi
Name:fbiBUniRule annotation
Ordered Locus Names:MLBr00758Imported
OrganismiMycobacterium leprae (strain Br4923)Imported
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi561304.MLBr_00758.

Structurei

3D structure databases

ProteinModelPortaliB8ZQE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 253253F420-0:gamma-glutamyl ligaseUniRule annotationAdd
BLAST
Regioni254 – 457204UnknownUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CofE family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1478.
KOiK12234.
OMAiGEEDLFW.
OrthoDBiEOG6P8TS8.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZQE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSDSHRSA PSPEHGTAST IEILPVAGLP EFRPGDDLSA ALAAAAPWLR
60 70 80 90 100
DGDVVVVTSK VVSKCEGRLV PAPEDTRGRN ELRRKLINDE TIRVLARKGR
110 120 130 140 150
TLIIENGLGL VQAAAGVDGS NVGRGELALL PVNPDASAAV LRIGLRAMLG
160 170 180 190 200
VNVAVVITDT MGRAWRNGQT DVAIGAAGLA VLHNYSGAVD RYGNELVVTE
210 220 230 240 250
IAVADEVAAA TDLVKGKLTA MPVAVVRGLS PTDDGSTAQH LLRNGPDDLF
260 270 280 290 300
WLGTTEALEL GRQQAQLLRR SVRQFSDEPI AAELIETAVA EALTAPAPHH
310 320 330 340 350
TRPVRFVWLQ TPAVRTRLLD RMADKWRLDL ASDALPADAI AQRVARGQIL
360 370 380 390 400
YDAPEVIIPF MVPDGAHAYP DAARASAEHT MFIVAVGAAV QALLVALAVR
410 420 430 440 450
GLGSCWIGST IFADDLVRAE LELPADWEPL GAIAIGYAHE PTDLREPVRV

ADLLLRK
Length:457
Mass (Da):48,562
Last modified:March 3, 2009 - v1
Checksum:i42BEF5D8A3333327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR70852.1.
RefSeqiWP_010907911.1. NC_011896.1.
YP_002503218.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR70852; CAR70852; MLBr00758.
KEGGimlb:MLBr_00758.
PATRICi18042282. VBIMycLep121698_1372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211192 Genomic DNA. Translation: CAR70852.1.
RefSeqiWP_010907911.1. NC_011896.1.
YP_002503218.1. NC_011896.1.

3D structure databases

ProteinModelPortaliB8ZQE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561304.MLBr_00758.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR70852; CAR70852; MLBr00758.
KEGGimlb:MLBr_00758.
PATRICi18042282. VBIMycLep121698_1372.

Phylogenomic databases

eggNOGiCOG1478.
KOiK12234.
OMAiGEEDLFW.
OrthoDBiEOG6P8TS8.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMLEP561304:GJP6-773-MONOMER.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923Imported.

Entry informationi

Entry nameiB8ZQE1_MYCLB
AccessioniPrimary (citable) accession number: B8ZQE1
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: April 29, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.