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B8ZQE1

- B8ZQE1_MYCLB

UniProt

B8ZQE1 - B8ZQE1_MYCLB

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Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium leprae (strain Br4923)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation
Monovalent cation. The ion could be potassium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591GTPUniRule annotation
Binding sitei64 – 641GTPUniRule annotation
Metal bindingi118 – 1181Divalent metal cation 1UniRule annotation
Binding sitei121 – 1211GTPUniRule annotation
Metal bindingi159 – 1591Divalent metal cation 1UniRule annotation
Metal bindingi160 – 1601Divalent metal cation 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 324GTPUniRule annotation

GO - Molecular functioni

  1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
  2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
  3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. oxidoreductase activity Source: InterPro

GO - Biological processi

  1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
  2. F420-0 metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMLEP561304:GJP6-773-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligaseUniRule annotation
Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
Gene namesi
Name:fbiBUniRule annotation
Ordered Locus Names:MLBr00758Imported
OrganismiMycobacterium leprae (strain Br4923)Imported
Taxonomic identifieri561304 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000006900: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi561304.MLBr_00758.

Structurei

3D structure databases

ProteinModelPortaliB8ZQE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 253253F420-0:gamma-glutamyl ligaseUniRule annotationAdd
BLAST
Regioni254 – 457204UnknownUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CofE family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1478.
KOiK12234.
OMAiGEEDLFW.
OrthoDBiEOG6P8TS8.

Family and domain databases

Gene3Di3.40.109.10. 1 hit.
HAMAPiMF_01259. F420_ligase_FbiB.
InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view]
SUPFAMiSSF55469. SSF55469. 1 hit.
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZQE1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSSDSHRSA PSPEHGTAST IEILPVAGLP EFRPGDDLSA ALAAAAPWLR
60 70 80 90 100
DGDVVVVTSK VVSKCEGRLV PAPEDTRGRN ELRRKLINDE TIRVLARKGR
110 120 130 140 150
TLIIENGLGL VQAAAGVDGS NVGRGELALL PVNPDASAAV LRIGLRAMLG
160 170 180 190 200
VNVAVVITDT MGRAWRNGQT DVAIGAAGLA VLHNYSGAVD RYGNELVVTE
210 220 230 240 250
IAVADEVAAA TDLVKGKLTA MPVAVVRGLS PTDDGSTAQH LLRNGPDDLF
260 270 280 290 300
WLGTTEALEL GRQQAQLLRR SVRQFSDEPI AAELIETAVA EALTAPAPHH
310 320 330 340 350
TRPVRFVWLQ TPAVRTRLLD RMADKWRLDL ASDALPADAI AQRVARGQIL
360 370 380 390 400
YDAPEVIIPF MVPDGAHAYP DAARASAEHT MFIVAVGAAV QALLVALAVR
410 420 430 440 450
GLGSCWIGST IFADDLVRAE LELPADWEPL GAIAIGYAHE PTDLREPVRV

ADLLLRK
Length:457
Mass (Da):48,562
Last modified:March 3, 2009 - v1
Checksum:i42BEF5D8A3333327
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR70852.1.
RefSeqiWP_010907911.1. NC_011896.1.
YP_002503218.1. NC_011896.1.

Genome annotation databases

EnsemblBacteriaiCAR70852; CAR70852; MLBr00758.
GeneIDi7326230.
KEGGimlb:MLBr_00758.
PATRICi18042282. VBIMycLep121698_1372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM211192 Genomic DNA. Translation: CAR70852.1 .
RefSeqi WP_010907911.1. NC_011896.1.
YP_002503218.1. NC_011896.1.

3D structure databases

ProteinModelPortali B8ZQE1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561304.MLBr_00758.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR70852 ; CAR70852 ; MLBr00758 .
GeneIDi 7326230.
KEGGi mlb:MLBr_00758.
PATRICi 18042282. VBIMycLep121698_1372.

Phylogenomic databases

eggNOGi COG1478.
KOi K12234.
OMAi GEEDLFW.
OrthoDBi EOG6P8TS8.

Enzyme and pathway databases

UniPathwayi UPA00071 .
BioCyci MLEP561304:GJP6-773-MONOMER.

Family and domain databases

Gene3Di 3.40.109.10. 1 hit.
HAMAPi MF_01259. F420_ligase_FbiB.
InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR019943. F420_FbiB_C.
IPR023661. F420_gamma-glut_ligase_bact.
IPR029479. Nitroreductase.
IPR000415. Nitroreductase-like.
[Graphical view ]
Pfami PF01996. F420_ligase. 1 hit.
PF00881. Nitroreductase. 1 hit.
[Graphical view ]
SUPFAMi SSF55469. SSF55469. 1 hit.
TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
TIGR03553. F420_FbiB_CTERM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Br4923Imported.

Entry informationi

Entry nameiB8ZQE1_MYCLB
AccessioniPrimary (citable) accession number: B8ZQE1
Entry historyi
Integrated into UniProtKB/TrEMBL: March 3, 2009
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3