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B8ZQE1

- B8ZQE1_MYCLB

UniProt

B8ZQE1 - B8ZQE1_MYCLB

Protein

Coenzyme F420:L-glutamate ligase

Gene

fbiB

Organism
Mycobacterium leprae (strain Br4923)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives.UniRule annotation

    Catalytic activityi

    GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
    GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.UniRule annotation
    GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation
    Monovalent cation. The ion could be potassium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591GTPUniRule annotation
    Binding sitei64 – 641GTPUniRule annotation
    Metal bindingi118 – 1181Divalent metal cation 1UniRule annotation
    Binding sitei121 – 1211GTPUniRule annotation
    Metal bindingi159 – 1591Divalent metal cation 1UniRule annotation
    Metal bindingi160 – 1601Divalent metal cation 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 324GTPUniRule annotation

    GO - Molecular functioni

    1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
    2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
    3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
    2. F420-0 metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotation

    Keywords - Ligandi

    GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

    Enzyme and pathway databases

    BioCyciMLEP561304:GJP6-773-MONOMER.
    UniPathwayiUPA00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
    Alternative name(s):
    Coenzyme F420-0:L-glutamate ligaseUniRule annotation
    Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
    Gene namesi
    Name:fbiBUniRule annotation
    Ordered Locus Names:MLBr00758Imported
    OrganismiMycobacterium leprae (strain Br4923)Imported
    Taxonomic identifieri561304 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000006900: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi561304.MLBr_00758.

    Structurei

    3D structure databases

    ProteinModelPortaliB8ZQE1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 253253F420-0:gamma-glutamyl ligaseUniRule annotationAdd
    BLAST
    Regioni254 – 457204UnknownUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the CofE family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1478.
    KOiK12234.
    OMAiGEEDLFW.
    OrthoDBiEOG6P8TS8.

    Family and domain databases

    Gene3Di3.40.109.10. 1 hit.
    HAMAPiMF_01259. F420_ligase_FbiB.
    InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR019943. F420_FbiB_C.
    IPR023661. F420_gamma-glut_ligase_bact.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF01996. F420_ligase. 1 hit.
    PF00881. Nitroreductase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55469. SSF55469. 1 hit.
    TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
    TIGR03553. F420_FbiB_CTERM. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8ZQE1-1 [UniParc]FASTAAdd to Basket

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    MTSSDSHRSA PSPEHGTAST IEILPVAGLP EFRPGDDLSA ALAAAAPWLR    50
    DGDVVVVTSK VVSKCEGRLV PAPEDTRGRN ELRRKLINDE TIRVLARKGR 100
    TLIIENGLGL VQAAAGVDGS NVGRGELALL PVNPDASAAV LRIGLRAMLG 150
    VNVAVVITDT MGRAWRNGQT DVAIGAAGLA VLHNYSGAVD RYGNELVVTE 200
    IAVADEVAAA TDLVKGKLTA MPVAVVRGLS PTDDGSTAQH LLRNGPDDLF 250
    WLGTTEALEL GRQQAQLLRR SVRQFSDEPI AAELIETAVA EALTAPAPHH 300
    TRPVRFVWLQ TPAVRTRLLD RMADKWRLDL ASDALPADAI AQRVARGQIL 350
    YDAPEVIIPF MVPDGAHAYP DAARASAEHT MFIVAVGAAV QALLVALAVR 400
    GLGSCWIGST IFADDLVRAE LELPADWEPL GAIAIGYAHE PTDLREPVRV 450
    ADLLLRK 457
    Length:457
    Mass (Da):48,562
    Last modified:March 3, 2009 - v1
    Checksum:i42BEF5D8A3333327
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211192 Genomic DNA. Translation: CAR70852.1.
    RefSeqiWP_010907911.1. NC_011896.1.
    YP_002503218.1. NC_011896.1.

    Genome annotation databases

    EnsemblBacteriaiCAR70852; CAR70852; MLBr00758.
    GeneIDi7326230.
    KEGGimlb:MLBr_00758.
    PATRICi18042282. VBIMycLep121698_1372.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211192 Genomic DNA. Translation: CAR70852.1 .
    RefSeqi WP_010907911.1. NC_011896.1.
    YP_002503218.1. NC_011896.1.

    3D structure databases

    ProteinModelPortali B8ZQE1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561304.MLBr_00758.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR70852 ; CAR70852 ; MLBr00758 .
    GeneIDi 7326230.
    KEGGi mlb:MLBr_00758.
    PATRICi 18042282. VBIMycLep121698_1372.

    Phylogenomic databases

    eggNOGi COG1478.
    KOi K12234.
    OMAi GEEDLFW.
    OrthoDBi EOG6P8TS8.

    Enzyme and pathway databases

    UniPathwayi UPA00071 .
    BioCyci MLEP561304:GJP6-773-MONOMER.

    Family and domain databases

    Gene3Di 3.40.109.10. 1 hit.
    HAMAPi MF_01259. F420_ligase_FbiB.
    InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR019943. F420_FbiB_C.
    IPR023661. F420_gamma-glut_ligase_bact.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view ]
    Pfami PF01996. F420_ligase. 1 hit.
    PF00881. Nitroreductase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55469. SSF55469. 1 hit.
    TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
    TIGR03553. F420_FbiB_CTERM. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Br4923Imported.

    Entry informationi

    Entry nameiB8ZQE1_MYCLB
    AccessioniPrimary (citable) accession number: B8ZQE1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 3, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3