B8ZMJ8 (FMT_STRPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	SPN23F17380

Organism

Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1) [Complete proteome] [HAMAP]

Taxonomic identifier

561276 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the Fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000190044
Regions
	Region	110 – 113	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8ZMJ8 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 96A9E4FE6FBF7316	FASTA	311	33,971
	10 20 30 40 50 60 MTKLIFMGTP DFSATVLKGL LTDDRYEILA VVTQPDRAVG RKKVIQETPV KQAAKEAGLS 70 80 90 100 110 120 IYQPEKLSGS PEMEELMKLG ADGIVTAAFG QFLPSKLLDS MDFAVNVHAS LLPRHRGGAP 130 140 150 160 170 180 IHYALIQGDE EAGVTIMEMV KEMDAGDMIS RRSIPITDED NVGTLFEKLA LVGRDLLLDT 190 200 210 220 230 240 LPAYIAGDIK PEPQDTSQVT FSPNIKPEEE KLDWNKTNRQ LFNQIRGMNP WPVAHAFLKG 250 260 270 280 290 300 DRFKIYEALP VEGQGNPGEI LSIGKKELIV ATAEGALSLK QVQPAGKPKM DIASFLNGVG 310 RTLTVGERFG D

References

[1]

"Role of conjugative elements in the evolution of the multidrug-resistant pandemic clone Streptococcus pneumoniae Spain23F ST81." Croucher N.J., Walker D., Romero P., Lennard N., Paterson G.K., Bason N.C., Mitchell A.M., Quail M.A., Andrew P.W., Parkhill J., Bentley S.D., Mitchell T.J. J. Bacteriol. 191:1480-1489(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700669 / Spain 23F-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211187 Genomic DNA. Translation: CAR69505.1.
RefSeq	YP_002511621.1. NC_011900.1.
3D structure databases
ProteinModelPortal	B8ZMJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	561276.SPN23F_17380.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7329512.
KEGG	sne:SPN23F_17380.
PATRIC	19674852. VBIStrPne132160_1828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
KO	K00604.
OMA	DWNKSAR.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	SPNE561276:GJFJ-1665-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_STRPJ

Accession

Primary (citable) accession number: B8ZMJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 29, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program