B8ZLK0 (GATB_STRPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	SPN23F04090

Organism

Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1) [Complete proteome] [HAMAP]

Taxonomic identifier

561276 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_1000122537

Sequences

	Sequence		Length	Mass (Da)
	B8ZLK0 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: C21C477E1BD7FDBD	FASTA	480	53,716
	10 20 30 40 50 60 MNFETVIGLE VHVELNTNSK IFSPTSAHFG NDQNANTNVI DWSFPGVLPV LNKGVVDAGI 70 80 90 100 110 120 KAALALNMDI HKKMHFDRKN YFYPDNPKAY QISQFDEPIG YNGWIEVELE DGTTKKIGIE 130 140 150 160 170 180 RAHLEEDAGK NTHGTDGYSY VDLNRQGVPL IEIVSEADMR SPEEAYAYLT ALKEVIQYAG 190 200 210 220 230 240 ISDVKMEEGS MRVDANISLR PYGQEKFGTK TELKNLNSFS NVRKGLEYEV QRQAEILRSG 250 260 270 280 290 300 GQIRQETRRY DEANKATILM RVKEGAADYR YFPEPDLPLF EISDEWIEEM RTELPEFPKE 310 320 330 340 350 360 RRARYVSDLG LSDYDASQLT ANKVTSNFFE KAVALGGDAK QVSNWLQGEV AQFLNAEGKT 370 380 390 400 410 420 LEQIELTPEN LVEMITIIED GTISSKIAKK VFVHLAKNGG GAREYVEKAG MVQISDPAIL 430 440 450 460 470 480 IPIIHQVFAD NEAAVADFKS GKRNADKAFT GFLMRATKGQ ANPQVALKLL AQELAKLKEN

References

[1]

"Role of conjugative elements in the evolution of the multidrug-resistant pandemic clone Streptococcus pneumoniae Spain23F ST81." Croucher N.J., Walker D., Romero P., Lennard N., Paterson G.K., Bason N.C., Mitchell A.M., Quail M.A., Andrew P.W., Parkhill J., Bentley S.D., Mitchell T.J. J. Bacteriol. 191:1480-1489(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700669 / Spain 23F-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM211187 Genomic DNA. Translation: CAR68258.1.
RefSeq	YP_002510451.1. NC_011900.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	561276.SPN23F_04090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7329251.
KEGG	sne:SPN23F_04090.
PATRIC	19672080. VBIStrPne132160_0443.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_STRPJ

Accession

Primary (citable) accession number: B8ZLK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program