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B8ZKS5

- SYL_STRPJ

UniProt

B8ZKS5 - SYL_STRPJ

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei613 – 6131ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSPNE561276:GJFJ-246-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:SPN23F02440
    OrganismiStreptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
    Taxonomic identifieri561276 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000002600: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 833833Leucine--tRNA ligasePRO_1000199226Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi561276.SPN23F_02440.

    Structurei

    Secondary structure

    1
    833
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi229 – 2368
    Beta strandi242 – 2487
    Helixi250 – 2556
    Beta strandi258 – 2614
    Helixi268 – 2714
    Turni274 – 2763
    Helixi277 – 28812
    Helixi292 – 2965
    Beta strandi305 – 3128
    Turni314 – 3163
    Beta strandi319 – 3246
    Beta strandi335 – 3395
    Turni341 – 3433
    Helixi345 – 35410
    Turni367 – 3693
    Helixi382 – 3843
    Helixi389 – 40315
    Beta strandi405 – 4095

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4K47X-ray2.02A228-410[»]
    4K48X-ray2.49A228-410[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi41 – 5212"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiDKPKYYA.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8ZKS5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFYNHKEIE PKWQGYWAEH HTFKTGTDAS KPKFYALDMF PYPSGAGLHV    50
    GHPEGYTATD ILSRYKRAQG YNVLHPMGWD AFGLPAEQYA MDTGNDPAEF 100
    TAENIANFKR QINALGFSYD WDREVNTTDP NYYKWTQWIF TKLYEKGLAY 150
    EAEVPVNWVE ELGTAIANEE VLPDGTSERG GYPVVRKPMR QWMLKITAYA 200
    ERLLNDLDEL DWSESIKDMQ RNWIGKSTGA NVTFKVKGTD KEFTVFTTRP 250
    DTLFGATFTV LAPEHELVDA ITSSEQAEAV ADYKHQASLK SDLVRTDLAK 300
    EKTGVWTGAY AINPVNGKEM PIWIADYVLA SYGTGAVMAV PAHDQRDWEF 350
    AKQFDLPIVE VLEGGNVEEA AYTEDGLHVN SDFLDGLNKE DAIAKIVACL 400
    EEKGCGQEKV TYRLRDWLFS RQRYWGEPIP IIHWEDGTST AVPETELPLV 450
    LPVTKDIRPS GTGESPLANL TDWLEVTRED GVKGRRETNT MPQWAGSSWY 500
    YLRYIDPHNT EKLADEDLLK QWLPVDIYVG GAEHAVLHLL YARFWHKFLY 550
    DLGVVPTKEP FQKLFNQGMI LGTSYRDHRG ALVATDKVEK RDGSFFHIET 600
    GEELEQAPAK MSKSLKNVVN PDDVVEQYGA DTLRVYEMFM GPLDASIAWS 650
    EEGLEGSRKF LDRVYRLITS KEILAENNGA LDKAYNETVK AVTEQIESLK 700
    FNTAIAQLMV FVNAANKEDK LYVDYAKGFI QLIAPFAPHL AEELWQTVAE 750
    TGESISYVAW PTWDESKLVE DEIEIVVQIK GKVRAKLMVA KDLSREELQE 800
    IALADEKVKA EIDGKEIVKV ISVPNKLVNI VVK 833
    Length:833
    Mass (Da):94,339
    Last modified:March 3, 2009 - v1
    Checksum:iC9F166C999CFC3F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211187 Genomic DNA. Translation: CAR68104.1.
    RefSeqiYP_002510309.1. NC_011900.1.

    Genome annotation databases

    EnsemblBacteriaiCAR68104; CAR68104; SPN23F02440.
    GeneIDi7328595.
    KEGGisne:SPN23F_02440.
    PATRICi19671734. VBIStrPne132160_0270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM211187 Genomic DNA. Translation: CAR68104.1 .
    RefSeqi YP_002510309.1. NC_011900.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4K47 X-ray 2.02 A 228-410 [» ]
    4K48 X-ray 2.49 A 228-410 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561276.SPN23F_02440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR68104 ; CAR68104 ; SPN23F02440 .
    GeneIDi 7328595.
    KEGGi sne:SPN23F_02440.
    PATRICi 19671734. VBIStrPne132160_0270.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi DKPKYYA.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci SPNE561276:GJFJ-246-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Role of conjugative elements in the evolution of the multidrug-resistant pandemic clone Streptococcus pneumoniae Spain23F ST81."
      Croucher N.J., Walker D., Romero P., Lennard N., Paterson G.K., Bason N.C., Mitchell A.M., Quail M.A., Andrew P.W., Parkhill J., Bentley S.D., Mitchell T.J.
      J. Bacteriol. 191:1480-1489(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700669 / Spain 23F-1.

    Entry informationi

    Entry nameiSYL_STRPJ
    AccessioniPrimary (citable) accession number: B8ZKS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3