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Protein

UvrABC system protein B

Gene

uvrB

Organism
Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51ATP8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
Ordered Locus Names:SPN23F11340
OrganismiStreptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)
Taxonomic identifieri561276 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002005541 – 662UvrABC system protein BAdd BLAST662

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Structurei

3D structure databases

ProteinModelPortaliB8ZJQ9.
SMRiB8ZJQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 188Helicase ATP-bindingAdd BLAST158
Domaini435 – 601Helicase C-terminalAdd BLAST167
Domaini626 – 661UVRAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 120Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.

Sequence similaritiesi

Belongs to the UvrB family.

Phylogenomic databases

KOiK03702.
OMAiRYMHSEI.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

B8ZJQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINHITDNQF KLVSKYQPSG DQPQAIEQLV DNIEGGEKAQ ILMGATGTGK
60 70 80 90 100
TYTMSQVISK VNKPTLVIAH NKTLAGQLYG EFKEFFPENA VEYFVSYYDY
110 120 130 140 150
YQPEAYVPSS DTYIEKDSSV NDEIDKLRHS ATSALLERND VIVVASVSCI
160 170 180 190 200
YGLGSPKEYA DSVVSLRPGL EISRDKLLND LVDIQFERND IDFQRGRFRV
210 220 230 240 250
RGDVVEIFPA SRDEHAFRVE FFGDEIDRIR EVEALTGQVL GEVDHLAIFP
260 270 280 290 300
ATHFVTNDDH MEVAIAKIQA ELEEQLAVFE KEGKLLEAQR LKQRTEYDIE
310 320 330 340 350
MLREMGYTNG VENYSRHMDG RSEGEPPYTL LDFFPDDFLI MIDESHMTIG
360 370 380 390 400
QIKGMYNGDR SRKEMLVNYG FRLPSALDNR PLRREEFESH VHQIVYVSAT
410 420 430 440 450
PGDYENEQTE TVIEQIIRPT GLLDPEVEVR PTMGQIDDLL GEINARVEKN
460 470 480 490 500
ERTFITTLTK KMAEDLTDYF KEMGIKVKYM HSDIKTLERT EIIRDLRLGV
510 520 530 540 550
FDVLVGINLL REGIDVPEVS LVAILDADKE GFLRNERGLI QTIGRAARNS
560 570 580 590 600
EGHVIMYADT VTQSMQRAID ETARRRKIQM AYNEEHGIVP QTIKKEIRDL
610 620 630 640 650
IAVTKAVAKE EDKEVDINSL NKQERKELVK KLEKQMQEAV EVLDFELAAQ
660
IRDMMLEVKA LD
Length:662
Mass (Da):75,734
Last modified:March 3, 2009 - v1
Checksum:iC2EB617ED537BDD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM211187 Genomic DNA. Translation: CAR68943.1.
RefSeqiWP_000607027.1. NC_011900.1.

Genome annotation databases

EnsemblBacteriaiCAR68943; CAR68943; SPN23F11340.
KEGGisne:SPN23F11340.

Similar proteinsi

Entry informationi

Entry nameiUVRB_STRPJ
AccessioniPrimary (citable) accession number: B8ZJQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 3, 2009
Last modified: October 25, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families