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Protein

Santalene and bergamotene synthase, chloroplastic

Gene

SBS

Organism
Solanum habrochaites (Wild tomato) (Lycopersicon hirsutum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

(2Z,6Z)-farnesyl diphosphate cyclizing enzyme. Produces (+)-alpha-santalene, (+)-endo-beta-bergamotene, (-)-endo-alpha-bergamotene, and at lower amounts, (-)exo-alpha-bergamotene and (+)-epi-beta-santalene. Not able to use geranyl diphosphate, E,E-farnesyl diphosphate or E,E,E-geranylgeranyl diphosphate as substrates, but able to use Neryl diphosphate to make the monoterpene terpineol.1 Publication

Catalytic activityi

(2Z,6Z)-farnesyl diphosphate = (+)-alpha-santalene.1 Publication
(2Z,6Z)-farnesyl diphosphate = (+)-endo-beta-bergamotene.1 Publication
(2Z,6Z)-farnesyl diphosphate = (-)-endo-alpha-bergamotene.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi530 – 5301Magnesium or manganese 1By similarity
Metal bindingi530 – 5301Magnesium or manganese 2By similarity
Metal bindingi534 – 5341Magnesium or manganese 1By similarity
Metal bindingi534 – 5341Magnesium or manganese 2By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. terpene synthase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14819.

Names & Taxonomyi

Protein namesi
Recommended name:
Santalene and bergamotene synthase, chloroplastic
Alternative name(s):
(+)-alpha-santalene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing) (EC:4.2.3.50)
(+)-endo-beta-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing) (EC:4.2.3.53)
(-)-endo-alpha-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing) (EC:4.2.3.54)
Gene namesi
Name:SBS
OrganismiSolanum habrochaites (Wild tomato) (Lycopersicon hirsutum)
Taxonomic identifieri62890 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636ChloroplastSequence AnalysisAdd
BLAST
Chaini37 – 777741Santalene and bergamotene synthase, chloroplasticPRO_0000405118Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB8XA41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi530 – 5345DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity

Sequence similaritiesi

Belongs to the terpene synthase family. Tpse subfamily.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8XA41-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIVGYRSTII TLSHPKLGNG KTISSNAIFQ RSCRVRCSHS TPSSMNGFED
60 70 80 90 100
ARDRIRESFG KVELSPSSYD TAWVAMVPSK HSLNEPCFPQ CLDWIIENQR
110 120 130 140 150
EDGSWGLNPS HPLLLKDSLS STLACLLALT KWRVGDEQIK RGLGFIETQS
160 170 180 190 200
WAIDNKDQIS PLGFEIIFPS MIKSAEKLNL NLAINKRDST IKRALQNEFT
210 220 230 240 250
RNIEYMSEGV GELCDWKEII KLHQRQNGSL FDSPATTAAA LIYHQHDKKC
260 270 280 290 300
YEYLNSILQQ HKNWVPTMYP TKIHSLLCLV DTLQNLGVHR HFKSEIKKAL
310 320 330 340 350
DEIYRLWQQK NEQIFSNVTH CAMAFRLLRM SYYDVSSDEL AEFVDEEHFF
360 370 380 390 400
AISGKYTSHV EILELHKASQ LAIDHEKDDI LDKINNWTRT FMEQKLLNNG
410 420 430 440 450
FIDRMSKKEV ELALRKFYTI SDLAENRRCI KSYEENNFKI LKAAYRSPNI
460 470 480 490 500
YNKDLFIFSI RNFELCQAQH QEELQQFKRW FEDYRLDQLG IAERYIHDTY
510 520 530 540 550
LCAVIVVPEP ELSDARLLYA KYVLLLTIVD DQFDSFASTD ECLNIIELVE
560 570 580 590 600
RWDDYASVGY KSEKVKVFFS TLYKSIEELV TIAEIKQGRS VKNHLLNLWL
610 620 630 640 650
ELVKLMLMER VEWFSGKTIP SIEEYLYVTS ITFGARLIPL TTQYFLGIKI
660 670 680 690 700
SEDILESDEI YGLCNCTGRV LRILNDLQDS KKEQKEDSVT IVTLLMKSMS
710 720 730 740 750
EEEAIMKIKE ILEMNRRELL KMVLVQKKGS QLPQICKDIF WRTSNWADFI
760 770
YLQTDGYRIA EEMKNHIDEV FYKPLNH
Length:777
Mass (Da):90,626
Last modified:March 3, 2009 - v1
Checksum:iAE19AD4DA742979D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ194970 mRNA. Translation: ACJ38409.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ194970 mRNA. Translation: ACJ38409.1.

3D structure databases

ProteinModelPortaliB8XA41.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14819.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl pyrophosphate in the wild tomato Solanum habrochaites."
    Sallaud C., Rontein D., Onillon S., Jabes F., Duffe P., Giacalone C., Thoraval S., Escoffier C., Herbette G., Leonhardt N., Causse M., Tissier A.
    Plant Cell 21:301-317(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Trichome gland.

Entry informationi

Entry nameiSBS_SOLHA
AccessioniPrimary (citable) accession number: B8XA41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: March 3, 2009
Last modified: January 7, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.