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B8XA41 (SBS_SOLHA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Santalene and bergamotene synthase, chloroplastic
Alternative name(s):
(+)-alpha-santalene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.50
(+)-endo-beta-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.53
(-)-endo-alpha-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.54
Gene names
Name:SBS
OrganismSolanum habrochaites (Wild tomato) (Lycopersicon hirsutum)
Taxonomic identifier62890 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length777 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

(2Z,6Z)-farnesyl diphosphate cyclizing enzyme. Produces (+)-alpha-santalene, (+)-endo-beta-bergamotene, (-)-endo-alpha-bergamotene, and at lower amounts, (-)exo-alpha-bergamotene and (+)-epi-beta-santalene. Not able to use geranyl diphosphate, E,E-farnesyl diphosphate or E,E,E-geranylgeranyl diphosphate as substrates, but able to use Neryl diphosphate to make the monoterpene terpineol. Ref.1

Catalytic activity

(2Z,6Z)-farnesyl diphosphate = (+)-alpha-santalene. Ref.1

(2Z,6Z)-farnesyl diphosphate = (+)-endo-beta-bergamotene. Ref.1

(2Z,6Z)-farnesyl diphosphate = (-)-endo-alpha-bergamotene. Ref.1

Cofactor

Magnesium or manganese By similarity.

Subcellular location

Plastidchloroplast Ref.1.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.

Sequence similarities

Belongs to the terpene synthase family. Tpse subfamily.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
Gene Ontology (GO)
   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Chloroplast Potential
Chain37 – 777741Santalene and bergamotene synthase, chloroplastic
PRO_0000405118

Regions

Motif530 – 5345DDXXD motif

Sites

Metal binding5301Magnesium or manganese 1 By similarity
Metal binding5301Magnesium or manganese 2 By similarity
Metal binding5341Magnesium or manganese 1 By similarity
Metal binding5341Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8XA41 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: AE19AD4DA742979D

FASTA77790,626
        10         20         30         40         50         60 
MIVGYRSTII TLSHPKLGNG KTISSNAIFQ RSCRVRCSHS TPSSMNGFED ARDRIRESFG 

        70         80         90        100        110        120 
KVELSPSSYD TAWVAMVPSK HSLNEPCFPQ CLDWIIENQR EDGSWGLNPS HPLLLKDSLS 

       130        140        150        160        170        180 
STLACLLALT KWRVGDEQIK RGLGFIETQS WAIDNKDQIS PLGFEIIFPS MIKSAEKLNL 

       190        200        210        220        230        240 
NLAINKRDST IKRALQNEFT RNIEYMSEGV GELCDWKEII KLHQRQNGSL FDSPATTAAA 

       250        260        270        280        290        300 
LIYHQHDKKC YEYLNSILQQ HKNWVPTMYP TKIHSLLCLV DTLQNLGVHR HFKSEIKKAL 

       310        320        330        340        350        360 
DEIYRLWQQK NEQIFSNVTH CAMAFRLLRM SYYDVSSDEL AEFVDEEHFF AISGKYTSHV 

       370        380        390        400        410        420 
EILELHKASQ LAIDHEKDDI LDKINNWTRT FMEQKLLNNG FIDRMSKKEV ELALRKFYTI 

       430        440        450        460        470        480 
SDLAENRRCI KSYEENNFKI LKAAYRSPNI YNKDLFIFSI RNFELCQAQH QEELQQFKRW 

       490        500        510        520        530        540 
FEDYRLDQLG IAERYIHDTY LCAVIVVPEP ELSDARLLYA KYVLLLTIVD DQFDSFASTD 

       550        560        570        580        590        600 
ECLNIIELVE RWDDYASVGY KSEKVKVFFS TLYKSIEELV TIAEIKQGRS VKNHLLNLWL 

       610        620        630        640        650        660 
ELVKLMLMER VEWFSGKTIP SIEEYLYVTS ITFGARLIPL TTQYFLGIKI SEDILESDEI 

       670        680        690        700        710        720 
YGLCNCTGRV LRILNDLQDS KKEQKEDSVT IVTLLMKSMS EEEAIMKIKE ILEMNRRELL 

       730        740        750        760        770 
KMVLVQKKGS QLPQICKDIF WRTSNWADFI YLQTDGYRIA EEMKNHIDEV FYKPLNH 

« Hide

References

[1]"A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl pyrophosphate in the wild tomato Solanum habrochaites."
Sallaud C., Rontein D., Onillon S., Jabes F., Duffe P., Giacalone C., Thoraval S., Escoffier C., Herbette G., Leonhardt N., Causse M., Tissier A.
Plant Cell 21:301-317(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, FUNCTION.
Tissue: Trichome gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FJ194970 mRNA. Translation: ACJ38409.1.

3D structure databases

ProteinModelPortalB8XA41.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14819.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 3 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSBS_SOLHA
AccessionPrimary (citable) accession number: B8XA41
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: March 3, 2009
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families