B8XA41 (SBS_SOLHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Santalene and bergamotene synthase, chloroplastic

Alternative name(s):
(+)-alpha-santalene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.50
(+)-endo-beta-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.53
(-)-endo-alpha-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing)
EC=4.2.3.54

Gene names

Name:

SBS

Organism

Solanum habrochaites (Wild tomato) (Lycopersicon hirsutum)

Taxonomic identifier

62890 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	(2Z,6Z)-farnesyl diphosphate cyclizing enzyme. Produces (+)-alpha-santalene, (+)-endo-beta-bergamotene, (-)-endo-alpha-bergamotene, and at lower amounts, (-)exo-alpha-bergamotene and (+)-epi-beta-santalene. Not able to use geranyl diphosphate, E,E-farnesyl diphosphate or E,E,E-geranylgeranyl diphosphate as substrates, but able to use Neryl diphosphate to make the monoterpene terpineol. Ref.1
Catalytic activity	(2Z,6Z)-farnesyl diphosphate = (+)-alpha-santalene. Ref.1 (2Z,6Z)-farnesyl diphosphate = (+)-endo-beta-bergamotene. Ref.1 (2Z,6Z)-farnesyl diphosphate = (-)-endo-alpha-bergamotene. Ref.1
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Plastid › chloroplast Ref.1.
Domain	The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+ By similarity.
Sequence similarities	Belongs to the terpene synthase family. Tpse subfamily.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro terpene synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Chloroplast Potential
	Chain	37 – 777	741	Santalene and bergamotene synthase, chloroplastic		PRO_0000405118
Regions
	Motif	530 – 534	5	DDXXD motif
Sites
	Metal binding	530	1	Magnesium or manganese 1 By similarity
	Metal binding	530	1	Magnesium or manganese 2 By similarity
	Metal binding	534	1	Magnesium or manganese 1 By similarity
	Metal binding	534	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8XA41 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: AE19AD4DA742979D	FASTA	777	90,626
	10 20 30 40 50 60 MIVGYRSTII TLSHPKLGNG KTISSNAIFQ RSCRVRCSHS TPSSMNGFED ARDRIRESFG 70 80 90 100 110 120 KVELSPSSYD TAWVAMVPSK HSLNEPCFPQ CLDWIIENQR EDGSWGLNPS HPLLLKDSLS 130 140 150 160 170 180 STLACLLALT KWRVGDEQIK RGLGFIETQS WAIDNKDQIS PLGFEIIFPS MIKSAEKLNL 190 200 210 220 230 240 NLAINKRDST IKRALQNEFT RNIEYMSEGV GELCDWKEII KLHQRQNGSL FDSPATTAAA 250 260 270 280 290 300 LIYHQHDKKC YEYLNSILQQ HKNWVPTMYP TKIHSLLCLV DTLQNLGVHR HFKSEIKKAL 310 320 330 340 350 360 DEIYRLWQQK NEQIFSNVTH CAMAFRLLRM SYYDVSSDEL AEFVDEEHFF AISGKYTSHV 370 380 390 400 410 420 EILELHKASQ LAIDHEKDDI LDKINNWTRT FMEQKLLNNG FIDRMSKKEV ELALRKFYTI 430 440 450 460 470 480 SDLAENRRCI KSYEENNFKI LKAAYRSPNI YNKDLFIFSI RNFELCQAQH QEELQQFKRW 490 500 510 520 530 540 FEDYRLDQLG IAERYIHDTY LCAVIVVPEP ELSDARLLYA KYVLLLTIVD DQFDSFASTD 550 560 570 580 590 600 ECLNIIELVE RWDDYASVGY KSEKVKVFFS TLYKSIEELV TIAEIKQGRS VKNHLLNLWL 610 620 630 640 650 660 ELVKLMLMER VEWFSGKTIP SIEEYLYVTS ITFGARLIPL TTQYFLGIKI SEDILESDEI 670 680 690 700 710 720 YGLCNCTGRV LRILNDLQDS KKEQKEDSVT IVTLLMKSMS EEEAIMKIKE ILEMNRRELL 730 740 750 760 770 KMVLVQKKGS QLPQICKDIF WRTSNWADFI YLQTDGYRIA EEMKNHIDEV FYKPLNH

References

[1]

"A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl pyrophosphate in the wild tomato Solanum habrochaites." Sallaud C., Rontein D., Onillon S., Jabes F., Duffe P., Giacalone C., Thoraval S., Escoffier C., Herbette G., Leonhardt N., Causse M., Tissier A. Plant Cell 21:301-317(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, FUNCTION. Tissue: Trichome gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FJ194970 mRNA. Translation: ACJ38409.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14819.
Family and domain databases
Gene3D	1.10.600.10. 1 hit. 1.50.30.10. 1 hit.
InterPro	IPR001906. Terpene_synth_N. IPR005630. Terpene_synthase_metal-bd. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF01397. Terpene_synth. 1 hit. PF03936. Terpene_synth_C. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits. SSF48576. Terpenoid_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SBS_SOLHA

Accession

Primary (citable) accession number: B8XA41

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program