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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Penicillium expansum (Blue mold rot fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei261SubstrateBy similarity1
Active sitei299NucleophileSequence analysis1
Binding sitei365SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
OrganismiPenicillium expansum (Blue mold rot fungus)
Taxonomic identifieri27334 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicillium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039522219 – 1011Probable beta-galactosidase AAdd BLAST993

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi267 ↔ 316By similarity
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Glycosylationi625N-linked (GlcNAc...)Sequence analysis1
Glycosylationi763N-linked (GlcNAc...)Sequence analysis1
Glycosylationi780N-linked (GlcNAc...)Sequence analysis1
Glycosylationi917N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliB8QGZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8QGZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSSLALA ALAAQVSGAA ISHKLDGFTL REHADASKRE LLQKYVTWDK
60 70 80 90 100
HSIFINGERL MMFSGEVHPY RLPVASLYID VFEKIKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGHYT AEGIFDLQPF FDAAKEAGIY LLARPGPYIN AEVSGGGFPG
160 170 180 190 200
WLQRVNGTLR TSIGDYLKST DNYASHIAKT IAKAQITNGG PIILYQPENE
210 220 230 240 250
YSGACCGYED FPDGSYMQYI EDHARDAGIV VPFISNDAYA GGHNAPGTGK
260 270 280 290 300
GAVDIYGHDG YPLGFDCANP SVWPDGNLPT NYHTLHEEQS PTTPFSVVEF
310 320 330 340 350
QGGAFDPWGG VGFAKCAHLL NHEFERVFYK NDFSFGVTFF NLYMIFGGTN
360 370 380 390 400
WGNLGHPGGY TSYDYGSAIS ESRNVTREKY SELKLLGNFA KVSPGYVVAN
410 420 430 440 450
PGNLTTSKYT KTADLTVTPL LGESSSAGSF FVIRHSNYNS QASVKYQLTL
460 470 480 490 500
PTSAGELTIP QLGGELTLSG RDSKIHVTDY DVAGSNILYS TAEVFTWKKF
510 520 530 540 550
DNGKVLILYG GPGEHHEFAV TGASASSVIE GSSSGITSKK IGDALVVAWD
560 570 580 590 600
VSSKRRIIKI GDLKVFLLDR NSAYNYWVPH LPTKGKAPGY VSKKAIDSSI
610 620 630 640 650
IVKAGYLVRS AFLSGKDLHI QADFNATTPI EIIGAPSSAK NLVINGKKTK
660 670 680 690 700
TNVDDNGIWS ASVSYSTPEI HLPSLKDLKW QSIDTLPEVK NSYDDSAWTS
710 720 730 740 750
ADQPHTLNTA HELQTPTTLF SSDYGYHTGT LLYRGHFVAN GKESIFFIQT
760 770 780 790 800
QGGSAYGHSV WVNETYVGSW EGSGSNDNYN ATYTLPSLAT GKKYAITVVI
810 820 830 840 850
DNMGLDVNWV IGQEGMKNPR GIIRYNLAGH DASAISWKLT GNLGGEDYRD
860 870 880 890 900
TVRGPLNEGG LYAERQGFHQ PKPSTKNWDS SSPFTGLTKP GIRFYSASFD
910 920 930 940 950
LDLPSGYDIP LYFNFGNSTA PPDAYRVQLF VNGYQYGKYV NNVGPQTSFP
960 970 980 990 1000
VPEGILNYHG TNWIALSLWA QQESGAKLNS FELINTTPVL TSLDKVKSVD
1010
QPKYKSRKGA Y
Length:1,011
Mass (Da):110,414
Last modified:March 3, 2009 - v1
Checksum:i9362248E6B7B2552
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU543998 mRNA. Translation: ACD75821.1.
EU543999 Genomic DNA. Translation: ACD75822.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU543998 mRNA. Translation: ACD75821.1.
EU543999 Genomic DNA. Translation: ACD75822.1.

3D structure databases

ProteinModelPortaliB8QGZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGALA_PENEN
AccessioniPrimary (citable) accession number: B8QGZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 3, 2009
Last modified: September 7, 2016
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.