B8NXJ2 (XYNF3_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endo-1,4-beta-xylanase F3
Short name=Xylanase F3
EC=3.2.1.8

Alternative name(s):
1,4-beta-D-xylan xylanohydrolase F3

Gene names

Name:	xynF3
Synonyms:	xlnF3
ORF Names:	AFLA_008110

Organism

Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]

Taxonomic identifier

332952 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus flavus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Subcellular location	Secreted By similarity.
Induction	Expressed in presence of xylan and repressed by glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	endo-1,4-beta-xylanase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 324	305	Probable endo-1,4-beta-xylanase F3		PRO_0000393187
Sites
	Active site	155	1	Proton donor By similarity
	Active site	260	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	278 ↔ 284		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8NXJ2 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: AD8AEFAF8EDB4496	FASTA	324	35,239
	10 20 30 40 50 60 MVHLKALASG TLFASLASSA VISRQAAASI NDAFVAHGKK YFGTCSDQAL LQNSQNEAIV 70 80 90 100 110 120 RADFGQLTPE NSMKWDALEP SQGSFSFAGA DFLADYAKTN NKLVRGHTLV WHSQLPSWVQ 130 140 150 160 170 180 GITDKDTLTE VIKNHITTIM QRYKGQIYAW DVVNEIFDED GTLRDSVFSQ VLGEDFVRIA 190 200 210 220 230 240 FETAREADPN AKLYINDYNL DSADYAKTKG MVSYVKKWLD AGVPIDGIVS LLPPRDEGLT 250 260 270 280 290 300 SCTALTALAS TGVSEVAVTE LDIEGASSES YLEVVNACLD VSSCVGITVW GVSDKDSWRS 310 320 STSPLLFDSN YQAKDAYNAI IDAL

References

[1]

"Genome sequence of Aspergillus flavus strain NRRL 3357." Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ963486 Genomic DNA. Translation: EED44928.1.
RefSeq	XP_002385057.1. XM_002385016.1.
3D structure databases
ProteinModelPortal	B8NXJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	5059.CADAFLAP00012922.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFLAT00012922; CADAFLAP00012922; CADAFLAG00012922.
GeneID	7920217.
KEGG	afv:AFLA_008110.
Phylogenomic databases
eggNOG	COG3693.
HOGENOM	HOG000019847.
KO	K01181.
Enzyme and pathway databases
UniPathway	UPA00114.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNF3_ASPFN

Accession

Primary (citable) accession number: B8NXJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program