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B8NW36 (MANBB_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-mannosidase B

EC=3.2.1.25
Alternative name(s):
Mannanase B
Short name=Mannase B
Gene names
Name:mndB
ORF Names:AFLA_117830
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length844 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers manobiose over mannotriose and has no activity against polymeric mannan. Is also severly restricetd by galactosyl substitutions at the +1 subsite By similarity.

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathway

Glycan metabolism; N-glycan degradation.

Miscellaneous

In contrast to clade A beta-mannosidases, which are likely secreted, clade B proteins appear to be intracellular By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family. Beta-mannosidase B subfamily.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmannan catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 844844Beta-mannosidase B
PRO_0000394653

Sites

Active site4321Proton donor By similarity

Amino acid modifications

Glycosylation7231N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
B8NW36 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: CB0DFBDDBC8C0ADF

FASTA84496,003
        10         20         30         40         50         60 
MAAFSQYPLS TGWSFKDSDD QSPEAWMPVP VVPSVAHQDL QANQKLKNPY IGFNELDARW 

        70         80         90        100        110        120 
VNDKSWTYRT VFQKPAVAAG SSIILAFDGL DTFATVKLDG SVILQSDNMF LAHRVDVTKA 

       130        140        150        160        170        180 
LEAEGDHVLE IDFDCAMRRA RELREKDTKH NWASFNGDPA RMAVRKAQYH WGWDWGPLLS 

       190        200        210        220        230        240 
TAGIWREVRL EVYSAKISDL WTEVELASDH QTARVSAFTE VDAADSVDSY KASFLLSLHG 

       250        260        270        280        290        300 
KEVAREVATL KDKVAKVTFD VTQPSLWWPN GYGDPALYEI SVSLEKEDCE IHSVSKKIGI 

       310        320        330        340        350        360 
RTAELIQQPD RHGKSFFFRI NGVDVFCGGS CWIPADNLLP SITAERYRKW IELMVAGRQV 

       370        380        390        400        410        420 
MIRVWGGGCY EDDSFYQACD ELGVLVWQDF MFGCGNYPTW PELLESIEKE ANYNVRRLRH 

       430        440        450        460        470        480 
HPSIVVYVGN NEDYQVQESA GLVYDYEDKN PENWLKTDFP ARYIYEKLLP SVVEKLSPKT 

       490        500        510        520        530        540 
VYHPGSPWGD GKITSDPTVG DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI 

       550        560        570        580        590        600 
EYFVENEADK YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETYVYL TQVVQAETMM 

       610        620        630        640        650        660 
FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA RVLKPIAVGV 

       670        680        690        700        710        720 
RREHHDWSVT HAQPPKTSKY ELWIASSLQK ETVGTIELRF LSVNTGLDVR APILRDNVKI 

       730        740        750        760        770        780 
VPNGTTNILE GVINHKAQPE PHVLAARLWV DGEVTARDVD WPQPFKYLDL SDRGLEVNKV 

       790        800        810        820        830        840 
SESGNEQKLL ITAKKPVKCL VFEERDGIRV SDSAMDIVPG DGQTVTVTGL KAGDAPLKYK 


YLGQ 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963485 Genomic DNA. Translation: EED45554.1.
RefSeqXP_002384490.1. XM_002384449.1.

3D structure databases

ProteinModelPortalB8NW36.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5059.CADAFLAP00012355.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFLAT00012355; CADAFLAP00012355; CADAFLAG00012355.
GeneID7921506.
KEGGafv:AFLA_117830.

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000186861.
KOK01192.
OMAPSINWGV.
OrthoDBEOG7NSB9Q.

Enzyme and pathway databases

UniPathwayUPA00280.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMANBB_ASPFN
AccessionPrimary (citable) accession number: B8NW36
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries