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B8NW36

- MANBB_ASPFN

UniProt

B8NW36 - MANBB_ASPFN

Protein

Beta-mannosidase B

Gene

mndB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers manobiose over mannotriose and has no activity against polymeric mannan. Is also severly restricetd by galactosyl substitutions at the +1 subsite By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei432 – 4321Proton donorBy similarity

    GO - Molecular functioni

    1. beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mannan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    UniPathwayiUPA00280.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mannosidase B (EC:3.2.1.25)
    Alternative name(s):
    Mannanase B
    Short name:
    Mannase B
    Gene namesi
    Name:mndB
    ORF Names:AFLA_117830
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 844844Beta-mannosidase BPRO_0000394653Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi723 – 7231N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5059.CADAFLAP00012355.

    Structurei

    3D structure databases

    ProteinModelPortaliB8NW36.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000186861.
    KOiK01192.
    OMAiPSINWGV.
    OrthoDBiEOG7NSB9Q.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view]
    PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8NW36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAFSQYPLS TGWSFKDSDD QSPEAWMPVP VVPSVAHQDL QANQKLKNPY    50
    IGFNELDARW VNDKSWTYRT VFQKPAVAAG SSIILAFDGL DTFATVKLDG 100
    SVILQSDNMF LAHRVDVTKA LEAEGDHVLE IDFDCAMRRA RELREKDTKH 150
    NWASFNGDPA RMAVRKAQYH WGWDWGPLLS TAGIWREVRL EVYSAKISDL 200
    WTEVELASDH QTARVSAFTE VDAADSVDSY KASFLLSLHG KEVAREVATL 250
    KDKVAKVTFD VTQPSLWWPN GYGDPALYEI SVSLEKEDCE IHSVSKKIGI 300
    RTAELIQQPD RHGKSFFFRI NGVDVFCGGS CWIPADNLLP SITAERYRKW 350
    IELMVAGRQV MIRVWGGGCY EDDSFYQACD ELGVLVWQDF MFGCGNYPTW 400
    PELLESIEKE ANYNVRRLRH HPSIVVYVGN NEDYQVQESA GLVYDYEDKN 450
    PENWLKTDFP ARYIYEKLLP SVVEKLSPKT VYHPGSPWGD GKITSDPTVG 500
    DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI EYFVENEADK 550
    YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETYVYL TQVVQAETMM 600
    FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA 650
    RVLKPIAVGV RREHHDWSVT HAQPPKTSKY ELWIASSLQK ETVGTIELRF 700
    LSVNTGLDVR APILRDNVKI VPNGTTNILE GVINHKAQPE PHVLAARLWV 750
    DGEVTARDVD WPQPFKYLDL SDRGLEVNKV SESGNEQKLL ITAKKPVKCL 800
    VFEERDGIRV SDSAMDIVPG DGQTVTVTGL KAGDAPLKYK YLGQ 844
    Length:844
    Mass (Da):96,003
    Last modified:March 3, 2009 - v1
    Checksum:iCB0DFBDDBC8C0ADF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963485 Genomic DNA. Translation: EED45554.1.
    RefSeqiXP_002384490.1. XM_002384449.1.

    Genome annotation databases

    EnsemblFungiiCADAFLAT00012355; CADAFLAP00012355; CADAFLAG00012355.
    GeneIDi7921506.
    KEGGiafv:AFLA_117830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963485 Genomic DNA. Translation: EED45554.1 .
    RefSeqi XP_002384490.1. XM_002384449.1.

    3D structure databases

    ProteinModelPortali B8NW36.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5059.CADAFLAP00012355.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFLAT00012355 ; CADAFLAP00012355 ; CADAFLAG00012355 .
    GeneIDi 7921506.
    KEGGi afv:AFLA_117830.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000186861.
    KOi K01192.
    OMAi PSINWGV.
    OrthoDBi EOG7NSB9Q.

    Enzyme and pathway databases

    UniPathwayi UPA00280 .

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view ]
    PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiMANBB_ASPFN
    AccessioniPrimary (citable) accession number: B8NW36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to clade A beta-mannosidases, which are likely secreted, clade B proteins appear to be intracellular.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3