SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B8NW36

- MANBB_ASPFN

UniProt

B8NW36 - MANBB_ASPFN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Beta-mannosidase B
Gene
mndB, AFLA_117830
Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of beta-mannosidic oligosaccharides of various complexity and length. Prefers manobiose over mannotriose and has no activity against polymeric mannan. Is also severly restricetd by galactosyl substitutions at the +1 subsite By similarity.

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei432 – 4321Proton donor By similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00280.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase B (EC:3.2.1.25)
Alternative name(s):
Mannanase B
Short name:
Mannase B
Gene namesi
Name:mndB
ORF Names:AFLA_117830
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000001875: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 844844Beta-mannosidase B
PRO_0000394653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi723 – 7231N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5059.CADAFLAP00012355.

Structurei

3D structure databases

ProteinModelPortaliB8NW36.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000186861.
KOiK01192.
OMAiPSINWGV.
OrthoDBiEOG7NSB9Q.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

B8NW36-1 [UniParc]FASTAAdd to Basket

« Hide

MAAFSQYPLS TGWSFKDSDD QSPEAWMPVP VVPSVAHQDL QANQKLKNPY    50
IGFNELDARW VNDKSWTYRT VFQKPAVAAG SSIILAFDGL DTFATVKLDG 100
SVILQSDNMF LAHRVDVTKA LEAEGDHVLE IDFDCAMRRA RELREKDTKH 150
NWASFNGDPA RMAVRKAQYH WGWDWGPLLS TAGIWREVRL EVYSAKISDL 200
WTEVELASDH QTARVSAFTE VDAADSVDSY KASFLLSLHG KEVAREVATL 250
KDKVAKVTFD VTQPSLWWPN GYGDPALYEI SVSLEKEDCE IHSVSKKIGI 300
RTAELIQQPD RHGKSFFFRI NGVDVFCGGS CWIPADNLLP SITAERYRKW 350
IELMVAGRQV MIRVWGGGCY EDDSFYQACD ELGVLVWQDF MFGCGNYPTW 400
PELLESIEKE ANYNVRRLRH HPSIVVYVGN NEDYQVQESA GLVYDYEDKN 450
PENWLKTDFP ARYIYEKLLP SVVEKLSPKT VYHPGSPWGD GKITSDPTVG 500
DMHQWNVWHG TQEKYQIFDT LGGRFNSEFG MEAFPHMSTI EYFVENEADK 550
YPQSHVLDFH NKADGHERRI ATYLVENLRT ATDLETYVYL TQVVQAETMM 600
FGYRGWRRQW GDERHCGGAL LWQLNDCWPT ISWAIVDYFL RPKPAFYAVA 650
RVLKPIAVGV RREHHDWSVT HAQPPKTSKY ELWIASSLQK ETVGTIELRF 700
LSVNTGLDVR APILRDNVKI VPNGTTNILE GVINHKAQPE PHVLAARLWV 750
DGEVTARDVD WPQPFKYLDL SDRGLEVNKV SESGNEQKLL ITAKKPVKCL 800
VFEERDGIRV SDSAMDIVPG DGQTVTVTGL KAGDAPLKYK YLGQ 844
Length:844
Mass (Da):96,003
Last modified:March 3, 2009 - v1
Checksum:iCB0DFBDDBC8C0ADF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963485 Genomic DNA. Translation: EED45554.1.
RefSeqiXP_002384490.1. XM_002384449.1.

Genome annotation databases

EnsemblFungiiCADAFLAT00012355; CADAFLAP00012355; CADAFLAG00012355.
GeneIDi7921506.
KEGGiafv:AFLA_117830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963485 Genomic DNA. Translation: EED45554.1 .
RefSeqi XP_002384490.1. XM_002384449.1.

3D structure databases

ProteinModelPortali B8NW36.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5059.CADAFLAP00012355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFLAT00012355 ; CADAFLAP00012355 ; CADAFLAG00012355 .
GeneIDi 7921506.
KEGGi afv:AFLA_117830.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000186861.
KOi K01192.
OMAi PSINWGV.
OrthoDBi EOG7NSB9Q.

Enzyme and pathway databases

UniPathwayi UPA00280 .

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view ]
PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
Pfami PF00703. Glyco_hydro_2. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
    Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Entry informationi

Entry nameiMANBB_ASPFN
AccessioniPrimary (citable) accession number: B8NW36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to clade A beta-mannosidases, which are likely secreted, clade B proteins appear to be intracellular By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi