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B8NVK8 (MANA_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannan endo-1,4-beta-mannosidase A

EC=3.2.1.78
Alternative name(s):
Endo-beta-1,4-mannanase A
Gene names
Name:manA
Synonyms:man1
ORF Names:AFLA_116950
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Sequence caution

The sequence EED45466.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 386365Probable mannan endo-1,4-beta-mannosidase A
PRO_0000393702

Sites

Active site2081Proton donor By similarity
Active site3161Nucleophile By similarity

Amino acid modifications

Glycosylation3361N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
B8NVK8 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: F30ED415E9A34993

FASTA38642,008
        10         20         30         40         50         60 
MKLNPSLLTA AGLVSAQLAS ALPQASSSTV SPSPSPSATP GSFVTTSGLN FVIDGKTGYF 

        70         80         90        100        110        120 
AGSNSYWIGF QKNNDDVDLV FSHLQESGLK ILRVWGFNDV NQKPTDGSVY YHLLADGTAT 

       130        140        150        160        170        180 
VNEGEDGLQR LDYVVSSAEK HGIKLIINFV NFWDDYGGIN AYVKAFGGSK EDFYTNDAMQ 

       190        200        210        220        230        240 
AAYRAYIKAV ISRYSDSTAI FAWELANEPR CQGCETTVLY NWIESTSQYI KSLDSKHLVC 

       250        260        270        280        290        300 
IGDEGFGLDT GSDGSYPYQY SEGSDFAKNL AIPTIDFGTF HLYPSSWGTT NDWGNGWVTS 

       310        320        330        340        350        360 
HGAACKEAGK PCLFEEYGVT SDHCAVEKPW QNTALNTTAI SGDLYWQYGD QLSGGPSPDD 

       370        380 
GNTFYYGTDD FKCLVTDHIA AINSRN 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963485 Genomic DNA. Translation: EED45466.1. Different initiation.
RefSeqXP_002384402.1. XM_002384361.1.

3D structure databases

ProteinModelPortalB8NVK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5059.CADAFLAP00012267.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7909308.
KEGGafv:AFLA_116950.

Phylogenomic databases

eggNOGCOG3934.
HOGENOMHOG000169951.
OrthoDBEOG7M3J90.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMANA_ASPFN
AccessionPrimary (citable) accession number: B8NVK8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: March 19, 2014
This is version 29 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries