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B8NSV5 (CREB_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable ubiquitin carboxyl-terminal hydrolase creB

EC=3.4.19.12
Alternative name(s):
Carbon catabolite repression protein B
Deubiquitinating enzyme creB
Ubiquitin thioesterase creB
Ubiquitin-hydrolyzing enzyme creB
Ubiquitin-specific-processing protease creB
Gene names
Name:creB
ORF Names:AFLA_051480
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin thioesterase component of the regulatory network controlling carbon source utilization through ubiquitination and deubiquitination involving creA, creB, creC, creD and acrB. Deubiquitinates the creA catabolic repressor and the quinate permease qutD. Plays also a role in response to carbon starvation and the control of extracellular proteases activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with creA, creC and qutD By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 755755Probable ubiquitin carboxyl-terminal hydrolase creB
PRO_0000395679

Regions

Domain55 – 468414USP
Coiled coil581 – 63050 Potential
Compositional bias676 – 6816Poly-Ser

Sites

Active site641Nucleophile By similarity
Active site4191Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
B8NSV5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: FA0FB9C8256630C4

FASTA75585,064
        10         20         30         40         50         60 
MGSFLRSLRR DVGPPTPSVG ATPAKKEPPV PPVTPLEKML QDMGAIREDG SDKFFGMENY 

        70         80         90        100        110        120 
GNTCYCNSIL QCLYYSVPFR EAVVNYPTRT PIESLEAALA NTLRYQNFAA NLEAEALAEK 

       130        140        150        160        170        180 
QKAANAQRPG APPNQPQKPE DKDSPEYKKK MALQTLPLLE TKNNATSYGM SESLFTSLKD 

       190        200        210        220        230        240 
LFESVVASQS RIGIIRPQHF LDVLRREHEM FRTAMHQDAH EFLNLLLNEV VANVEAEASK 

       250        260        270        280        290        300 
QPEPERSLPP AESADSTELS GSSGSKTPNT TRWVHELFEG TLTSETQCLT CEKVSQRDEV 

       310        320        330        340        350        360 
FLDLSVDLEQ HSSVTSCLRK FSAEEMLCER NKFHCDNCGG LQEAEKRMKI KRLPRILALH 

       370        380        390        400        410        420 
LKRFKYTEDL QRLQKLFHRV VYPYHLRLFN TTDDAEDPDR LYELYAVVVH IGGGPYHGHY 

       430        440        450        460        470        480 
VAIIKTQDRG WLLFDDEMVE PVDKNYVRNF FGDRPGLACA YVLFYQETTL EAVMKEQEQE 

       490        500        510        520        530        540 
NMDLNTSVAD INDSTLKQNG YPLSPGLAHV HSASQIPSPS EPARFSNLQR APTAPPLFPH 

       550        560        570        580        590        600 
PEHADSESSP ADPSTTASAT PPVPPIPDIH SLPLSPKKSD SHFKKERAKE EKERKANEKE 

       610        620        630        640        650        660 
KEKQRRRDQE ARIREQRRED AEIRAALEAS KASKAEEDRR HSPDDTKKSS HGLSRLKRGS 

       670        680        690        700        710        720 
KSFSHRLGKD KENRVSSSSH SATPIAEHPP SRNGASESQQ QLPNGQSPGS HGLHTRHTGL 

       730        740        750 
DEERDTLKDP KHDRSGHHGK WRSFSLKKKS FSILS 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963483 Genomic DNA. Translation: EED47094.1.
RefSeqXP_002383274.1. XM_002383233.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5059.CADAFLAP00011139.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFLAT00011139; CADAFLAP00011139; CADAFLAG00011139.
GeneID7909143.
KEGGafv:AFLA_051480.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000192482.
KOK11872.
OMARREHEMF.
OrthoDBEOG7TF7JV.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCREB_ASPFN
AccessionPrimary (citable) accession number: B8NSV5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries