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Protein

Probable pectinesterase A

Gene

pmeA

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in maceration and soft-rotting of plant tissue.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (AFLA_023340), Probable pectinesterase A (pmeA), Pectinesterase (AFLA_095140), Pectinesterase (AFLA_020000), Pectinesterase (AFLA_095150), Pectinesterase (AFLA_100100)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei142 – 1421SubstrateBy similarity
Sitei164 – 1641Transition state stabilizerBy similarity
Active sitei165 – 1651Proton donorPROSITE-ProRule annotation
Active sitei186 – 1861NucleophilePROSITE-ProRule annotation
Binding sitei246 – 2461SubstrateBy similarity
Binding sitei248 – 2481SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase A (EC:3.1.1.11)
Alternative name(s):
Pectin methylesterase A
Gene namesi
Name:pmeA
ORF Names:AFLA_001410
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_001410.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 324305Probable pectinesterase APRO_0000394080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217409.
KOiK01051.
OMAiCNIKAAS.
OrthoDBiEOG7CG78N.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8NPS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGSLLKLAL LSFSLASSAA VLPRDTGRTS APSGCSTVGT SGDYSTIGDA
60 70 80 90 100
LTALGSSTAD ACIYIAAGTY EEQLVINYAG HLTLYGETTD TQTYKQNTVT
110 120 130 140 150
ITHTISSPEA GSLDNSATVN IKSDLVSVYN INIANGYGSG AQAVALVANA
160 170 180 190 200
DQLGFYACQF TGYQDTLYAK AGHQYYINSR IEGAVDYIFG DASAWFENCD
210 220 230 240 250
IVSNGAGYIT AMSRETTSDT AWYAIDHCNI KAASGVDLTG DVYLGRPWRV
260 270 280 290 300
LARVIYQYSV LPDIINAKGW HSMADGATPL YYEFNNTGAG SDTSDREYLS
310 320
TIDAPVAKET VLGDDYKNWV DSSY
Length:324
Mass (Da):34,576
Last modified:March 3, 2009 - v1
Checksum:iA22F23BFC9AE3537
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963482 Genomic DNA. Translation: EED47500.1.
RefSeqiXP_002382342.1. XM_002382301.1.

Genome annotation databases

EnsemblFungiiCADAFLAT00010207; CADAFLAP00010207; CADAFLAG00010207.
GeneIDi7921854.
KEGGiafv:AFLA_001410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963482 Genomic DNA. Translation: EED47500.1.
RefSeqiXP_002382342.1. XM_002382301.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFLAT00010207; CADAFLAP00010207; CADAFLAG00010207.
GeneIDi7921854.
KEGGiafv:AFLA_001410.

Organism-specific databases

EuPathDBiFungiDB:AFLA_001410.

Phylogenomic databases

HOGENOMiHOG000217409.
KOiK01051.
OMAiCNIKAAS.
OrthoDBiEOG7CG78N.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes aflatoxin contamination of food and feed."
    Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E., Bhatnagar D., Bennett J.W., Dean R., Payne G.A.
    Genome Announc. 3:E0016815-E0016815(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Entry informationi

Entry nameiPMEA_ASPFN
AccessioniPrimary (citable) accession number: B8NPS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: March 3, 2009
Last modified: March 16, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.