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B8NKI4 (BGALB_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable beta-galactosidase B

EC=3.2.1.23
Alternative name(s):
Lactase B
Gene names
Name:lacB
ORF Names:AFLA_091500
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1020 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Sequence caution

The sequence EED49069.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 1020998Probable beta-galactosidase B
PRO_0000395224

Sites

Active site1961Proton donor Potential
Active site3071Nucleophile Potential
Binding site901Substrate By similarity
Binding site1351Substrate By similarity
Binding site1361Substrate; via amide nitrogen By similarity
Binding site1371Substrate By similarity
Binding site1951Substrate By similarity
Binding site2641Substrate By similarity
Binding site3721Substrate By similarity

Amino acid modifications

Glycosylation251N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation2101N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation4101N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential
Glycosylation5491N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential
Glycosylation6251N-linked (GlcNAc...) Potential
Glycosylation7021N-linked (GlcNAc...) Potential
Glycosylation7471N-linked (GlcNAc...) Potential
Glycosylation7851N-linked (GlcNAc...) Potential
Glycosylation8191N-linked (GlcNAc...) Potential
Glycosylation8801N-linked (GlcNAc...) Potential
Glycosylation9191N-linked (GlcNAc...) Potential
Disulfide bond270 ↔ 323 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8NKI4 [UniParc].

Last modified July 13, 2010. Version 2.
Checksum: 353EB4A96CBFBF8C

FASTA1,020112,288
        10         20         30         40         50         60 
MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN GQRHFVFSGE 

        70         80         90        100        110        120 
FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP GVLDFENGAH NFTSIMTLAK 

       130        140        150        160        170        180 
EIGLYMIIRP GPYVNAEANA GGLPLWTTTG AYGKLRDNDP RYLEALTPYW ANISKIIAPH 

       190        200        210        220        230        240 
LITNGGNVIL YQIENEYAEQ WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM 

       250        260        270        280        290        300 
NGHSWSKDLS NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYKTLIY YDYFKELSPT 

       310        320        330        340        350        360 
QPSFMPEFQG GSYNPWGGPQ GGCPDDLGPD FANLFYRNLI SQRVSAISLY MLYGGTNWGW 

       370        380        390        400        410        420 
HASTDVATSY DYSSPISENR KLIEKYYETK VLTQFTKIAQ DLSKVDRLGN STKYSSNPAV 

       430        440        450        460        470        480 
SVAELRNPDT GAAFYVTQHE YTPSGTVEKF TVKVNTSEGA LTIPQYGSQI TLNGHQSKII 

       490        500        510        520        530        540 
VTDFKFGSKT LLYSTAEVLT YAVIDGKEVL ALWVPTGESG EFTVKGVNSA KFADKGRTAN 

       550        560        570        580        590        600 
IEIHPGANNV TVSFMQRSGM SLVELGDGTR IVLLDRSAAH VFWSTPLNND PAEAGNNTVL 

       610        620        630        640        650        660 
VHGPYLVRSA KLEGCDLKLT GDIQNSTEVS IFAPKSVCSV NWNGKKTSVK SAKGGVITTT 

       670        680        690        700        710        720 
LGGDAKFELP TISGWKSADS LPEIAKDYSA TSKAWVVATK TNSSNPTPPA PNNPVLYVDE 

       730        740        750        760        770        780 
NDIHVGNHIY RATFPSTDEP PTDVYLNITG GRAFSYSVWL NSDFIGSWLG TATTEQNDQT 

       790        800        810        820        830        840 
FSFSNATLST DEDNILVVVM DNSAHDLRDG ALNPRGITNA TLIGPGSYSF TEWKLAGNAG 

       850        860        870        880        890        900 
FEDHLDPVRA PLNEGSLYAE RVGIHLPGYE FDEAEEVSSN STSLTVPGAG IRVFRTVVPL 

       910        920        930        940        950        960 
SVPQGLDVSI SFRLTAPSNV TFTSAEGYTN QLRALLFVNG YQYGRFNPYI GHQIDFPVPP 

       970        980        990       1000       1010       1020 
GVLDYNGDNT IAVTVWSQSV DGAEIKVDWN VDYVHETSFD MNFDGAYLRP GWIEERREYA 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963480 Genomic DNA. Translation: EED49069.1. Sequence problems.
RefSeqXP_002380970.1. XM_002380929.1.

3D structure databases

ProteinModelPortalB8NKI4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7920299.
KEGGafv:AFLA_091500.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000181922.
OrthoDBEOG7ZGXBD.

Family and domain databases

Gene3D2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGALB_ASPFN
AccessionPrimary (citable) accession number: B8NKI4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: April 16, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries