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B8NKI4

- BGALB_ASPFN

UniProt

B8NKI4 - BGALB_ASPFN

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Protein
Probable beta-galactosidase B
Gene
lacB, AFLA_091500
Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901Substrate By similarity
Binding sitei135 – 1351Substrate By similarity
Binding sitei136 – 1361Substrate; via amide nitrogen By similarity
Binding sitei137 – 1371Substrate By similarity
Binding sitei195 – 1951Substrate By similarity
Active sitei196 – 1961Proton donor Reviewed prediction
Binding sitei264 – 2641Substrate By similarity
Active sitei307 – 3071Nucleophile Reviewed prediction
Binding sitei372 – 3721Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AFLA_091500
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000001875: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 1020998Probable beta-galactosidase B
PRO_0000395224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi25 – 251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi172 – 1721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi210 – 2101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi251 – 2511N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi270 ↔ 323 By similarity
Glycosylationi271 – 2711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi410 – 4101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi455 – 4551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi549 – 5491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi596 – 5961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi625 – 6251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi702 – 7021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi747 – 7471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi785 – 7851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi819 – 8191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi880 – 8801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi919 – 9191N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliB8NKI4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8NKI4-1 [UniParc]FASTAAdd to Basket

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MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN     50
GQRHFVFSGE FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP 100
GVLDFENGAH NFTSIMTLAK EIGLYMIIRP GPYVNAEANA GGLPLWTTTG 150
AYGKLRDNDP RYLEALTPYW ANISKIIAPH LITNGGNVIL YQIENEYAEQ 200
WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM NGHSWSKDLS 250
NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYKTLIY YDYFKELSPT 300
QPSFMPEFQG GSYNPWGGPQ GGCPDDLGPD FANLFYRNLI SQRVSAISLY 350
MLYGGTNWGW HASTDVATSY DYSSPISENR KLIEKYYETK VLTQFTKIAQ 400
DLSKVDRLGN STKYSSNPAV SVAELRNPDT GAAFYVTQHE YTPSGTVEKF 450
TVKVNTSEGA LTIPQYGSQI TLNGHQSKII VTDFKFGSKT LLYSTAEVLT 500
YAVIDGKEVL ALWVPTGESG EFTVKGVNSA KFADKGRTAN IEIHPGANNV 550
TVSFMQRSGM SLVELGDGTR IVLLDRSAAH VFWSTPLNND PAEAGNNTVL 600
VHGPYLVRSA KLEGCDLKLT GDIQNSTEVS IFAPKSVCSV NWNGKKTSVK 650
SAKGGVITTT LGGDAKFELP TISGWKSADS LPEIAKDYSA TSKAWVVATK 700
TNSSNPTPPA PNNPVLYVDE NDIHVGNHIY RATFPSTDEP PTDVYLNITG 750
GRAFSYSVWL NSDFIGSWLG TATTEQNDQT FSFSNATLST DEDNILVVVM 800
DNSAHDLRDG ALNPRGITNA TLIGPGSYSF TEWKLAGNAG FEDHLDPVRA 850
PLNEGSLYAE RVGIHLPGYE FDEAEEVSSN STSLTVPGAG IRVFRTVVPL 900
SVPQGLDVSI SFRLTAPSNV TFTSAEGYTN QLRALLFVNG YQYGRFNPYI 950
GHQIDFPVPP GVLDYNGDNT IAVTVWSQSV DGAEIKVDWN VDYVHETSFD 1000
MNFDGAYLRP GWIEERREYA 1020
Length:1,020
Mass (Da):112,288
Last modified:July 13, 2010 - v2
Checksum:i353EB4A96CBFBF8C
GO

Sequence cautioni

The sequence EED49069.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963480 Genomic DNA. Translation: EED49069.1. Sequence problems.
RefSeqiXP_002380970.1. XM_002380929.1.

Genome annotation databases

GeneIDi7920299.
KEGGiafv:AFLA_091500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963480 Genomic DNA. Translation: EED49069.1 . Sequence problems.
RefSeqi XP_002380970.1. XM_002380929.1.

3D structure databases

ProteinModelPortali B8NKI4.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7920299.
KEGGi afv:AFLA_091500.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
    Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Entry informationi

Entry nameiBGALB_ASPFN
AccessioniPrimary (citable) accession number: B8NKI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: April 16, 2014
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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