Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B8NKI4

- BGALB_ASPFN

UniProt

B8NKI4 - BGALB_ASPFN

Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 2 (13 Jul 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei135 – 1351SubstrateBy similarity
    Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei195 – 1951SubstrateBy similarity
    Active sitei196 – 1961Proton donorSequence Analysis
    Binding sitei264 – 2641SubstrateBy similarity
    Active sitei307 – 3071NucleophileSequence Analysis
    Binding sitei372 – 3721SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase B (EC:3.2.1.23)
    Alternative name(s):
    Lactase B
    Gene namesi
    Name:lacB
    ORF Names:AFLA_091500
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 1020998Probable beta-galactosidase BPRO_0000395224Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi25 – 251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi270 ↔ 323By similarity
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi549 – 5491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi625 – 6251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi702 – 7021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi747 – 7471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi785 – 7851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi819 – 8191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi919 – 9191N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliB8NKI4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8NKI4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN     50
    GQRHFVFSGE FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP 100
    GVLDFENGAH NFTSIMTLAK EIGLYMIIRP GPYVNAEANA GGLPLWTTTG 150
    AYGKLRDNDP RYLEALTPYW ANISKIIAPH LITNGGNVIL YQIENEYAEQ 200
    WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM NGHSWSKDLS 250
    NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYKTLIY YDYFKELSPT 300
    QPSFMPEFQG GSYNPWGGPQ GGCPDDLGPD FANLFYRNLI SQRVSAISLY 350
    MLYGGTNWGW HASTDVATSY DYSSPISENR KLIEKYYETK VLTQFTKIAQ 400
    DLSKVDRLGN STKYSSNPAV SVAELRNPDT GAAFYVTQHE YTPSGTVEKF 450
    TVKVNTSEGA LTIPQYGSQI TLNGHQSKII VTDFKFGSKT LLYSTAEVLT 500
    YAVIDGKEVL ALWVPTGESG EFTVKGVNSA KFADKGRTAN IEIHPGANNV 550
    TVSFMQRSGM SLVELGDGTR IVLLDRSAAH VFWSTPLNND PAEAGNNTVL 600
    VHGPYLVRSA KLEGCDLKLT GDIQNSTEVS IFAPKSVCSV NWNGKKTSVK 650
    SAKGGVITTT LGGDAKFELP TISGWKSADS LPEIAKDYSA TSKAWVVATK 700
    TNSSNPTPPA PNNPVLYVDE NDIHVGNHIY RATFPSTDEP PTDVYLNITG 750
    GRAFSYSVWL NSDFIGSWLG TATTEQNDQT FSFSNATLST DEDNILVVVM 800
    DNSAHDLRDG ALNPRGITNA TLIGPGSYSF TEWKLAGNAG FEDHLDPVRA 850
    PLNEGSLYAE RVGIHLPGYE FDEAEEVSSN STSLTVPGAG IRVFRTVVPL 900
    SVPQGLDVSI SFRLTAPSNV TFTSAEGYTN QLRALLFVNG YQYGRFNPYI 950
    GHQIDFPVPP GVLDYNGDNT IAVTVWSQSV DGAEIKVDWN VDYVHETSFD 1000
    MNFDGAYLRP GWIEERREYA 1020
    Length:1,020
    Mass (Da):112,288
    Last modified:July 13, 2010 - v2
    Checksum:i353EB4A96CBFBF8C
    GO

    Sequence cautioni

    The sequence EED49069.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963480 Genomic DNA. Translation: EED49069.1. Sequence problems.
    RefSeqiXP_002380970.1. XM_002380929.1.

    Genome annotation databases

    GeneIDi7920299.
    KEGGiafv:AFLA_091500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963480 Genomic DNA. Translation: EED49069.1 . Sequence problems.
    RefSeqi XP_002380970.1. XM_002380929.1.

    3D structure databases

    ProteinModelPortali B8NKI4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7920299.
    KEGGi afv:AFLA_091500.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiBGALB_ASPFN
    AccessioniPrimary (citable) accession number: B8NKI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 36 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3