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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei136Substrate; via amide nitrogenBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei264SubstrateBy similarity1
Active sitei307NucleophileSequence analysis1
Binding sitei372SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AFLA_091500
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_091500.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039522423 – 1020Probable beta-galactosidase BAdd BLAST998

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi210N-linked (GlcNAc...)Sequence analysis1
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi270 ↔ 323By similarity
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1
Glycosylationi455N-linked (GlcNAc...)Sequence analysis1
Glycosylationi549N-linked (GlcNAc...)Sequence analysis1
Glycosylationi596N-linked (GlcNAc...)Sequence analysis1
Glycosylationi625N-linked (GlcNAc...)Sequence analysis1
Glycosylationi702N-linked (GlcNAc...)Sequence analysis1
Glycosylationi747N-linked (GlcNAc...)Sequence analysis1
Glycosylationi785N-linked (GlcNAc...)Sequence analysis1
Glycosylationi819N-linked (GlcNAc...)Sequence analysis1
Glycosylationi880N-linked (GlcNAc...)Sequence analysis1
Glycosylationi919N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliB8NKI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8NKI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISKTVLSG LALGASFVGV SAQQNSTRWP LHDNGLTDTV EWDHYSFLIN
60 70 80 90 100
GQRHFVFSGE FHYWRIPVPE LWRDLLEKIK AAGFTAFSIY NHWGYHSPKP
110 120 130 140 150
GVLDFENGAH NFTSIMTLAK EIGLYMIIRP GPYVNAEANA GGLPLWTTTG
160 170 180 190 200
AYGKLRDNDP RYLEALTPYW ANISKIIAPH LITNGGNVIL YQIENEYAEQ
210 220 230 240 250
WLDEETHEPN TSGQEYMQYL EDVARENGID APLIHNLPNM NGHSWSKDLS
260 270 280 290 300
NATGNVDVIG VDSYPTCWTC NVSECASTNG EYIPYKTLIY YDYFKELSPT
310 320 330 340 350
QPSFMPEFQG GSYNPWGGPQ GGCPDDLGPD FANLFYRNLI SQRVSAISLY
360 370 380 390 400
MLYGGTNWGW HASTDVATSY DYSSPISENR KLIEKYYETK VLTQFTKIAQ
410 420 430 440 450
DLSKVDRLGN STKYSSNPAV SVAELRNPDT GAAFYVTQHE YTPSGTVEKF
460 470 480 490 500
TVKVNTSEGA LTIPQYGSQI TLNGHQSKII VTDFKFGSKT LLYSTAEVLT
510 520 530 540 550
YAVIDGKEVL ALWVPTGESG EFTVKGVNSA KFADKGRTAN IEIHPGANNV
560 570 580 590 600
TVSFMQRSGM SLVELGDGTR IVLLDRSAAH VFWSTPLNND PAEAGNNTVL
610 620 630 640 650
VHGPYLVRSA KLEGCDLKLT GDIQNSTEVS IFAPKSVCSV NWNGKKTSVK
660 670 680 690 700
SAKGGVITTT LGGDAKFELP TISGWKSADS LPEIAKDYSA TSKAWVVATK
710 720 730 740 750
TNSSNPTPPA PNNPVLYVDE NDIHVGNHIY RATFPSTDEP PTDVYLNITG
760 770 780 790 800
GRAFSYSVWL NSDFIGSWLG TATTEQNDQT FSFSNATLST DEDNILVVVM
810 820 830 840 850
DNSAHDLRDG ALNPRGITNA TLIGPGSYSF TEWKLAGNAG FEDHLDPVRA
860 870 880 890 900
PLNEGSLYAE RVGIHLPGYE FDEAEEVSSN STSLTVPGAG IRVFRTVVPL
910 920 930 940 950
SVPQGLDVSI SFRLTAPSNV TFTSAEGYTN QLRALLFVNG YQYGRFNPYI
960 970 980 990 1000
GHQIDFPVPP GVLDYNGDNT IAVTVWSQSV DGAEIKVDWN VDYVHETSFD
1010 1020
MNFDGAYLRP GWIEERREYA
Length:1,020
Mass (Da):112,288
Last modified:July 13, 2010 - v2
Checksum:i353EB4A96CBFBF8C
GO

Sequence cautioni

The sequence EED49069 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963480 Genomic DNA. Translation: EED49069.1. Sequence problems.
RefSeqiXP_002380970.1. XM_002380929.1.

Genome annotation databases

EnsemblFungiiEED49069; EED49069; AFLA_091500.
GeneIDi7920299.
KEGGiafv:AFLA_091500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963480 Genomic DNA. Translation: EED49069.1. Sequence problems.
RefSeqiXP_002380970.1. XM_002380929.1.

3D structure databases

ProteinModelPortaliB8NKI4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEED49069; EED49069; AFLA_091500.
GeneIDi7920299.
KEGGiafv:AFLA_091500.

Organism-specific databases

EuPathDBiFungiDB:AFLA_091500.

Phylogenomic databases

HOGENOMiHOG000181922.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_ASPFN
AccessioniPrimary (citable) accession number: B8NKI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: November 2, 2016
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.