B8NIV9 (MANF_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannan endo-1,4-beta-mannosidase F
EC=3.2.1.78

Alternative name(s):
Endo-beta-1,4-mannanase F

Gene names

Name:	manF
ORF Names:	AFLA_069870

Organism

Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]

Taxonomic identifier

332952 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus flavus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.
Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted By similarity.
Domain	Has a modular structure: a carbohydrate-binding module (CBM) at the N-terminus, a linker rich in serines, and a C-terminal endo-1,4-mannanase catalytic module. The genes for catalytic modules and CBMs seem to have evolved separately and have been linked by gene fusion.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulose binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 463	445	Probable mannan endo-1,4-beta-mannosidase F		PRO_0000393714
Regions
	Domain	19 – 54	36	CBM1
	Region	75 – 118	44	Ser-rich linker
	Region	119 – 463	345	Catalytic
Sites
	Active site	286	1	Proton donor By similarity
	Active site	395	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	B8NIV9 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: 9DBA89743B6D4063	FASTA	463	49,994
	10 20 30 40 50 60 MRSLSSIALL SVVGAASAQA GPWAQCGGKS FSGSSECASG WKCQELNEWF SQCVPGAEST 70 80 90 100 110 120 TPTVSSTPTP TDAPSVSITA SATTGINKSI SVSSASKSTP LPSSSSASPS PRPTGSGSFA 130 140 150 160 170 180 KADGLQFSID GETKYFAGTN AYWLPFQMND ADIDSVFDHL EQAGLKILRV WGFNDVNTAP 190 200 210 220 230 240 SPGTVYFQLH DKEKSTSTIN TGKDGLQRLD YVVAAAEKHG VKLIIPFVNS WDDYGGYNAY 250 260 270 280 290 300 VKAYGGSKTE WFTNEKIQSV YQAYIKAVVS RYRDSPAIFA WELGNEPRCS GCSTDVIHGW 310 320 330 340 350 360 ATKISAYIKS LDPNHMVALG DEGMGLTIGS DQSYPYGTSE GNDFEKNLAI PDIDFGTLHL 370 380 390 400 410 420 YTTDWGIKDN AWGNGWVENH AKACKAAGKP CLFEEYGMKG NHCTDELKWQ KTSLSSGTAA 430 440 450 460 DLIWQYGQQL STGESPKDAY SIFYGTDEWK CAVMDHMENV NKN

References

[1]

"Genome sequence of Aspergillus flavus strain NRRL 3357." Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ963479 Genomic DNA. Translation: EED50165.1.
RefSeq	XP_002380546.1. XM_002380505.1.
3D structure databases
ProteinModelPortal	B8NIV9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFLAT00008411; CADAFLAP00008411; CADAFLAG00008411.
GeneID	7914152.
KEGG	afv:AFLA_069870.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169951.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000254. Cellulose-bd_dom_fun. IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANF_ASPFN

Accession

Primary (citable) accession number: B8NIV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program