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Protein

Probable endo-1,3(4)-beta-glucanase AFLA_105200

Gene

AFLA_105200

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates.By similarity

Catalytic activityi

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei144NucleophileBy similarity1
Active sitei149Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endo-1,3(4)-beta-glucanase AFLA_105200 (EC:3.2.1.6)
Alternative name(s):
Mixed-linked glucanase AFLA_105200
Gene namesi
ORF Names:AFLA_105200
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_105200.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000039508625 – 644Probable endo-1,3(4)-beta-glucanase AFLA_105200Add BLAST620
PropeptideiPRO_0000395087645 – 667Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Lipidationi644GPI-anchor amidated glycineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 288GH16PROSITE-ProRule annotationAdd BLAST264

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi583 – 615Ser-richAdd BLAST33

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000166269.
OMAiSCVNYVA.
OrthoDBiEOG092C3D34.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8N7S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSSFVWTV GSIALSSLIT PTIADGSGSR YQLTEAWQGE KFLDHFKFFS
60 70 80 90 100
GSDPTNGFVT YANQSYAESS GLIEVTESGS FYMGVDYKTK LSPNGPGRDS
110 120 130 140 150
VRIESKEYYD EGLYIIDLQH MPGSVCGTWP AFWSVGPNWP YDGEIDIIEG
160 170 180 190 200
VNKHEANEIV LHTSGSCSLS SENDMSGTMS SSECGESSGT IGCVVKGQTG
210 220 230 240 250
TSGAPFNEKN GGVYAMEWTS SFVKIWYFAR SEIPQSITEG NPDTTAFGTP
260 270 280 290 300
MAHLQGTCDF GERFKSQKFI LDTTFCGDWA GGVFGDSGCP VSDPSNPIQS
310 320 330 340 350
CVNYVAENPA AFKEAYWEIN YIKLFQTGTG HSTASIASQA ETATAVVSKT
360 370 380 390 400
VDSVPSVTST PILETTAPAP ETVSAEAPAT SSAVPEPANP QTSVAGAETT
410 420 430 440 450
AAPAPSPETT AAPASPSSDD SEGADAVSET TIYVTETTTI CGASTQKGTI
460 470 480 490 500
QTIGGGETEV SPASSTVESA ATPAAPTPTS QEPVASLPGT TVNDGTPVPT
510 520 530 540 550
DVSPETPAEE TAGESGAPTP SAEQPEKPQP AATSIETGIV PPPVSNPAPT
560 570 580 590 600
EQGTPEGASP VDATESRHVP DEPAPTSAAP IRSPSPSSWT ISSSSRVALS
610 620 630 640 650
SSFASTTSSA SRTTSATKEA TAPTETDSGA STGTNPESPV FTAGASKSVG
660
ISGLAGIVCG IAMAMLA
Length:667
Mass (Da):68,433
Last modified:March 3, 2009 - v1
Checksum:i6FA9DBFF4C651501
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963475 Genomic DNA. Translation: EED53146.1.
RefSeqiXP_002376392.1. XM_002376351.1.

Genome annotation databases

EnsemblFungiiEED53146; EED53146; AFLA_105200.
GeneIDi7922059.
KEGGiafv:AFLA_105200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963475 Genomic DNA. Translation: EED53146.1.
RefSeqiXP_002376392.1. XM_002376351.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEED53146; EED53146; AFLA_105200.
GeneIDi7922059.
KEGGiafv:AFLA_105200.

Organism-specific databases

EuPathDBiFungiDB:AFLA_105200.

Phylogenomic databases

HOGENOMiHOG000166269.
OMAiSCVNYVA.
OrthoDBiEOG092C3D34.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGLX_ASPFN
AccessioniPrimary (citable) accession number: B8N7S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.