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B8N7S7 (EGLX_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable endo-1,3(4)-beta-glucanase AFLA_105200

EC=3.2.1.6
Alternative name(s):
Mixed-linked glucanase AFLA_105200
Gene names
ORF Names:AFLA_105200
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length667 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates By similarity.

Catalytic activity

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 644620Probable endo-1,3(4)-beta-glucanase AFLA_105200
PRO_0000395086
Propeptide645 – 66723Removed in mature form Potential
PRO_0000395087

Regions

Compositional bias583 – 61533Ser-rich

Sites

Active site1441Nucleophile By similarity
Active site1491Proton donor By similarity

Amino acid modifications

Lipidation6441GPI-anchor amidated glycine Potential
Glycosylation631N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
B8N7S7 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 6FA9DBFF4C651501

FASTA66768,433
        10         20         30         40         50         60 
MSSSSFVWTV GSIALSSLIT PTIADGSGSR YQLTEAWQGE KFLDHFKFFS GSDPTNGFVT 

        70         80         90        100        110        120 
YANQSYAESS GLIEVTESGS FYMGVDYKTK LSPNGPGRDS VRIESKEYYD EGLYIIDLQH 

       130        140        150        160        170        180 
MPGSVCGTWP AFWSVGPNWP YDGEIDIIEG VNKHEANEIV LHTSGSCSLS SENDMSGTMS 

       190        200        210        220        230        240 
SSECGESSGT IGCVVKGQTG TSGAPFNEKN GGVYAMEWTS SFVKIWYFAR SEIPQSITEG 

       250        260        270        280        290        300 
NPDTTAFGTP MAHLQGTCDF GERFKSQKFI LDTTFCGDWA GGVFGDSGCP VSDPSNPIQS 

       310        320        330        340        350        360 
CVNYVAENPA AFKEAYWEIN YIKLFQTGTG HSTASIASQA ETATAVVSKT VDSVPSVTST 

       370        380        390        400        410        420 
PILETTAPAP ETVSAEAPAT SSAVPEPANP QTSVAGAETT AAPAPSPETT AAPASPSSDD 

       430        440        450        460        470        480 
SEGADAVSET TIYVTETTTI CGASTQKGTI QTIGGGETEV SPASSTVESA ATPAAPTPTS 

       490        500        510        520        530        540 
QEPVASLPGT TVNDGTPVPT DVSPETPAEE TAGESGAPTP SAEQPEKPQP AATSIETGIV 

       550        560        570        580        590        600 
PPPVSNPAPT EQGTPEGASP VDATESRHVP DEPAPTSAAP IRSPSPSSWT ISSSSRVALS 

       610        620        630        640        650        660 
SSFASTTSSA SRTTSATKEA TAPTETDSGA STGTNPESPV FTAGASKSVG ISGLAGIVCG 


IAMAMLA 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963475 Genomic DNA. Translation: EED53146.1.
RefSeqXP_002376392.1. XM_002376351.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFLAT00004257; CADAFLAP00004257; CADAFLAG00004257.
GeneID7922059.
KEGGafv:AFLA_105200.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000166269.
OMASCVNYVA.
OrthoDBEOG7HB5KN.

Family and domain databases

Gene3D2.60.120.200. 2 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 2 hits.
ProtoNetSearch...

Entry information

Entry nameEGLX_ASPFN
AccessionPrimary (citable) accession number: B8N7S7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries