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B8N7S7

- EGLX_ASPFN

UniProt

B8N7S7 - EGLX_ASPFN

Protein

Probable endo-1,3(4)-beta-glucanase AFLA_105200

Gene

AFLA_105200

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates.By similarity

    Catalytic activityi

    Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei144 – 1441NucleophileBy similarity
    Active sitei149 – 1491Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
    2. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable endo-1,3(4)-beta-glucanase AFLA_105200 (EC:3.2.1.6)
    Alternative name(s):
    Mixed-linked glucanase AFLA_105200
    Gene namesi
    ORF Names:AFLA_105200
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 644620Probable endo-1,3(4)-beta-glucanase AFLA_105200PRO_0000395086Add
    BLAST
    Propeptidei645 – 66723Removed in mature formSequence AnalysisPRO_0000395087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Lipidationi644 – 6441GPI-anchor amidated glycineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi583 – 61533Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000166269.
    OMAiSCVNYVA.
    OrthoDBiEOG7HB5KN.

    Family and domain databases

    Gene3Di2.60.120.200. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8N7S7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSSFVWTV GSIALSSLIT PTIADGSGSR YQLTEAWQGE KFLDHFKFFS    50
    GSDPTNGFVT YANQSYAESS GLIEVTESGS FYMGVDYKTK LSPNGPGRDS 100
    VRIESKEYYD EGLYIIDLQH MPGSVCGTWP AFWSVGPNWP YDGEIDIIEG 150
    VNKHEANEIV LHTSGSCSLS SENDMSGTMS SSECGESSGT IGCVVKGQTG 200
    TSGAPFNEKN GGVYAMEWTS SFVKIWYFAR SEIPQSITEG NPDTTAFGTP 250
    MAHLQGTCDF GERFKSQKFI LDTTFCGDWA GGVFGDSGCP VSDPSNPIQS 300
    CVNYVAENPA AFKEAYWEIN YIKLFQTGTG HSTASIASQA ETATAVVSKT 350
    VDSVPSVTST PILETTAPAP ETVSAEAPAT SSAVPEPANP QTSVAGAETT 400
    AAPAPSPETT AAPASPSSDD SEGADAVSET TIYVTETTTI CGASTQKGTI 450
    QTIGGGETEV SPASSTVESA ATPAAPTPTS QEPVASLPGT TVNDGTPVPT 500
    DVSPETPAEE TAGESGAPTP SAEQPEKPQP AATSIETGIV PPPVSNPAPT 550
    EQGTPEGASP VDATESRHVP DEPAPTSAAP IRSPSPSSWT ISSSSRVALS 600
    SSFASTTSSA SRTTSATKEA TAPTETDSGA STGTNPESPV FTAGASKSVG 650
    ISGLAGIVCG IAMAMLA 667
    Length:667
    Mass (Da):68,433
    Last modified:March 3, 2009 - v1
    Checksum:i6FA9DBFF4C651501
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963475 Genomic DNA. Translation: EED53146.1.
    RefSeqiXP_002376392.1. XM_002376351.1.

    Genome annotation databases

    EnsemblFungiiCADAFLAT00004257; CADAFLAP00004257; CADAFLAG00004257.
    GeneIDi7922059.
    KEGGiafv:AFLA_105200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963475 Genomic DNA. Translation: EED53146.1 .
    RefSeqi XP_002376392.1. XM_002376351.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFLAT00004257 ; CADAFLAP00004257 ; CADAFLAG00004257 .
    GeneIDi 7922059.
    KEGGi afv:AFLA_105200.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000166269.
    OMAi SCVNYVA.
    OrthoDBi EOG7HB5KN.

    Family and domain databases

    Gene3Di 2.60.120.200. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiEGLX_ASPFN
    AccessioniPrimary (citable) accession number: B8N7S7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3