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B8N7I7

- ABFB_ASPFN

UniProt

B8N7I7 - ABFB_ASPFN

Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei184 – 1852Cis-peptide bondBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Active sitei229 – 2291NucleophileBy similarity
    Binding sitei230 – 2301Substrate; via amide nitrogenBy similarity
    Binding sitei304 – 3041Substrate; via amide nitrogenBy similarity
    Active sitei305 – 3051Proton donorBy similarity
    Binding sitei424 – 4241SubstrateBy similarity
    Binding sitei426 – 4261Substrate; via amide nitrogenBy similarity
    Binding sitei427 – 4271Substrate; via amide nitrogenBy similarity
    Binding sitei443 – 4431SubstrateBy similarity
    Binding sitei471 – 4711SubstrateBy similarity
    Binding sitei473 – 4731Substrate; via amide nitrogenBy similarity
    Binding sitei476 – 4761Substrate; via amide nitrogenBy similarity
    Binding sitei496 – 4961SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB

    GO - Biological processi

    1. arabinan catabolic process Source: UniProtKB-UniPathway
    2. arabinose metabolic process Source: UniProtKB
    3. L-arabinose metabolic process Source: InterPro
    4. xylan catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00667.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
    Short name:
    ABF B
    Short name:
    Arabinosidase B
    Gene namesi
    Name:abfB
    ORF Names:AFLA_104300
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 506480Probable alpha-L-arabinofuranosidase BPRO_0000394604Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi29 ↔ 39By similarity
    Disulfide bondi89 ↔ 94By similarity
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi184 ↔ 185By similarity
    Disulfide bondi409 ↔ 447By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5059.CADAFLAP00004167.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 343317CatalyticBy similarityAdd
    BLAST
    Regioni344 – 506163ABDBy similarityAdd
    BLAST

    Domaini

    Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 54 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG83819.
    HOGENOMiHOG000187007.
    OMAiNIVAAKY.
    OrthoDBiEOG7DFXNQ.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
    IPR007934. AbfB.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PfamiPF05270. AbfB. 1 hit.
    PF09206. ArabFuran-catal. 1 hit.
    [Graphical view]
    SUPFAMiSSF110221. SSF110221. 1 hit.
    SSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8N7I7-1 [UniParc]FASTAAdd to Basket

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    MSSGLSLERA CAVALGIVAS ASLVAAGPCD IYSSGGTPCV AAHSTTRALY    50
    SAYTGALYQV KRGSDGSTTD IAPLSAGGVA DAATQDSFCA NTTCLITIIY 100
    DQSGRGNHLT QAPPGGFNGP ESNGYDNLAS AVGAPVTLNG KKAYGVFMSP 150
    GTGYRNNAAS GTATGDEAEG MYAVLDGTHY NSACCFDYGN AEVSNTDTGN 200
    GHMEAIYYGD NTVWGSGAGS GPWIMADLEN GLFSGLSSKN NAGDPSISYR 250
    FVTAVVKGEA NQWSIRGANA ASGSLSTYYS GARPSASGYN PMSKEGAIIL 300
    GIGGDNSNGA QGTFYEGVMT SGYPSDATEN SVQADIVAAK YAIASLTSGP 350
    ALTVGSSISL QVTTAGYTTR YLAHDGSTVN TQVVSSSSTT ALKQQASWTV 400
    RTGLANSACF SFESVDTPGS YIRHYNFALL LNANDGTKQF YEDATFCPQA 450
    GLNGQGNSIR SWSYPTRYFR HYENVLYVAS NGGVQTFDAT TSFNDDVSWV 500
    VSTGFA 506
    Length:506
    Mass (Da):52,217
    Last modified:March 3, 2009 - v1
    Checksum:iC27B7EE289D8A3AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963475 Genomic DNA. Translation: EED53056.1.
    RefSeqiXP_002376302.1. XM_002376261.1.

    Genome annotation databases

    EnsemblFungiiCADAFLAT00004167; CADAFLAP00004167; CADAFLAG00004167.
    GeneIDi7912203.
    KEGGiafv:AFLA_104300.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963475 Genomic DNA. Translation: EED53056.1 .
    RefSeqi XP_002376302.1. XM_002376261.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5059.CADAFLAP00004167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFLAT00004167 ; CADAFLAP00004167 ; CADAFLAG00004167 .
    GeneIDi 7912203.
    KEGGi afv:AFLA_104300.

    Phylogenomic databases

    eggNOGi NOG83819.
    HOGENOMi HOG000187007.
    OMAi NIVAAKY.
    OrthoDBi EOG7DFXNQ.

    Enzyme and pathway databases

    UniPathwayi UPA00667 .

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR015289. A-L-arabinofuranosidase_B_cat.
    IPR007934. AbfB.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    Pfami PF05270. AbfB. 1 hit.
    PF09206. ArabFuran-catal. 1 hit.
    [Graphical view ]
    SUPFAMi SSF110221. SSF110221. 1 hit.
    SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiABFB_ASPFN
    AccessioniPrimary (citable) accession number: B8N7I7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3