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B8N7I7

- ABFB_ASPFN

UniProt

B8N7I7 - ABFB_ASPFN

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Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan By similarity.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei184 – 1852Cis-peptide bondBy similarity
Binding sitei227 – 2271SubstrateBy similarity
Active sitei229 – 2291NucleophileBy similarity
Binding sitei230 – 2301Substrate; via amide nitrogenBy similarity
Binding sitei304 – 3041Substrate; via amide nitrogenBy similarity
Active sitei305 – 3051Proton donorBy similarity
Binding sitei424 – 4241SubstrateBy similarity
Binding sitei426 – 4261Substrate; via amide nitrogenBy similarity
Binding sitei427 – 4271Substrate; via amide nitrogenBy similarity
Binding sitei443 – 4431SubstrateBy similarity
Binding sitei471 – 4711SubstrateBy similarity
Binding sitei473 – 4731Substrate; via amide nitrogenBy similarity
Binding sitei476 – 4761Substrate; via amide nitrogenBy similarity
Binding sitei496 – 4961SubstrateBy similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB

GO - Biological processi

  1. arabinan catabolic process Source: UniProtKB-UniPathway
  2. arabinose metabolic process Source: UniProtKB
  3. L-arabinose metabolic process Source: InterPro
  4. xylan catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:AFLA_104300
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000001875: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 506480Probable alpha-L-arabinofuranosidase BPRO_0000394604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 39By similarity
Disulfide bondi89 ↔ 94By similarity
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi184 ↔ 185By similarity
Disulfide bondi409 ↔ 447By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi5059.CADAFLAP00004167.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 343317CatalyticBy similarityAdd
BLAST
Regioni344 – 506163ABDBy similarityAdd
BLAST

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 54 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG83819.
HOGENOMiHOG000187007.
OMAiNIVAAKY.
OrthoDBiEOG7DFXNQ.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB.
IPR013320. ConA-like_dom.
[Graphical view]
PfamiPF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view]
SUPFAMiSSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8N7I7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSGLSLERA CAVALGIVAS ASLVAAGPCD IYSSGGTPCV AAHSTTRALY
60 70 80 90 100
SAYTGALYQV KRGSDGSTTD IAPLSAGGVA DAATQDSFCA NTTCLITIIY
110 120 130 140 150
DQSGRGNHLT QAPPGGFNGP ESNGYDNLAS AVGAPVTLNG KKAYGVFMSP
160 170 180 190 200
GTGYRNNAAS GTATGDEAEG MYAVLDGTHY NSACCFDYGN AEVSNTDTGN
210 220 230 240 250
GHMEAIYYGD NTVWGSGAGS GPWIMADLEN GLFSGLSSKN NAGDPSISYR
260 270 280 290 300
FVTAVVKGEA NQWSIRGANA ASGSLSTYYS GARPSASGYN PMSKEGAIIL
310 320 330 340 350
GIGGDNSNGA QGTFYEGVMT SGYPSDATEN SVQADIVAAK YAIASLTSGP
360 370 380 390 400
ALTVGSSISL QVTTAGYTTR YLAHDGSTVN TQVVSSSSTT ALKQQASWTV
410 420 430 440 450
RTGLANSACF SFESVDTPGS YIRHYNFALL LNANDGTKQF YEDATFCPQA
460 470 480 490 500
GLNGQGNSIR SWSYPTRYFR HYENVLYVAS NGGVQTFDAT TSFNDDVSWV

VSTGFA
Length:506
Mass (Da):52,217
Last modified:March 3, 2009 - v1
Checksum:iC27B7EE289D8A3AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963475 Genomic DNA. Translation: EED53056.1.
RefSeqiXP_002376302.1. XM_002376261.1.

Genome annotation databases

EnsemblFungiiCADAFLAT00004167; CADAFLAP00004167; CADAFLAG00004167.
GeneIDi7912203.
KEGGiafv:AFLA_104300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EQ963475 Genomic DNA. Translation: EED53056.1 .
RefSeqi XP_002376302.1. XM_002376261.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5059.CADAFLAP00004167.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFLAT00004167 ; CADAFLAP00004167 ; CADAFLAG00004167 .
GeneIDi 7912203.
KEGGi afv:AFLA_104300.

Phylogenomic databases

eggNOGi NOG83819.
HOGENOMi HOG000187007.
OMAi NIVAAKY.
OrthoDBi EOG7DFXNQ.

Enzyme and pathway databases

UniPathwayi UPA00667 .

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR015289. A-L-arabinofuranosidase_B_cat.
IPR007934. AbfB.
IPR013320. ConA-like_dom.
[Graphical view ]
Pfami PF05270. AbfB. 1 hit.
PF09206. ArabFuran-catal. 1 hit.
[Graphical view ]
SUPFAMi SSF110221. SSF110221. 1 hit.
SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
    Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Entry informationi

Entry nameiABFB_ASPFN
AccessioniPrimary (citable) accession number: B8N7I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: March 3, 2009
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3