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B8N6V7

- BGALA_ASPFN

UniProt

B8N6V7 - BGALA_ASPFN

Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
    Binding sitei142 – 1421SubstrateBy similarity
    Binding sitei199 – 1991SubstrateBy similarity
    Active sitei200 – 2001Proton donorSequence Analysis
    Binding sitei260 – 2601SubstrateBy similarity
    Active sitei298 – 2981NucleophileSequence Analysis
    Binding sitei364 – 3641SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase A (EC:3.2.1.23)
    Alternative name(s):
    Lactase A
    Gene namesi
    Name:lacA
    ORF Names:AFLA_017100
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 1005987Probable beta-galactosidase APRO_0000395216Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi205 ↔ 206By similarity
    Disulfide bondi266 ↔ 315By similarity
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi522 – 5221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi760 – 7601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi777 – 7771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi805 – 8051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5059.CADAFLAP00003595.

    Structurei

    3D structure databases

    ProteinModelPortaliB8N6V7.
    SMRiB8N6V7. Positions 41-1005.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi175 – 18410Poly-Ala

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OMAiHNAPGTG.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8N6V7-1 [UniParc]FASTAAdd to Basket

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    MKLLSVAAVA LLAAQAAGAS IKHRLNGFTI LEHPDPAKRD LLQDIVTWDD     50
    KSLFINGERI MLFSGEVHPF RLPVPSLWLD IFHKIRALGF NCVSFYIDWA 100
    LLEGKPGDYR AEGIFALEPF FDAAKEAGIY LIARPGSYIN AEVSGGGFPG 150
    WLQRVNGTLR SSDEPFLKAT DNYIANAAAA VAKAQITNGG PVILYQPENE 200
    YSGGCCGVKY PDADYMQYVM DQARKADIVV PFISNDASPS GHNAPGSGTG 250
    AVDIYGHDSY PLGFDCANPS VWPEGKLPDN FRTLHLEQSP STPYSLLEFQ 300
    AGAFDPWGGP GFEKCYALVN HEFSRVFYRN DLSFGVSTFN LYMTFGGTNW 350
    GNLGHPGGYT SYDYGSPITE TRNVTREKYS DIKLLANFVK ASPSYLTATP 400
    RNLTTGVYTD TSDLAVTPLI GDSPGSFFVV RHTDYSSQES TSYKLKLPTS 450
    AGNLTIPQLE GTLSLNGRDS KIHVVDYNVS GTNIIYSTAE VFTWKKFDGN 500
    KVLVLYGGPK EHHELAIASK SNVTIIEGSD SGIVSTRKGS SVIIGWDVSS 550
    TRRIVQVGDL RVFLLDRNSA YNYWVPELPT EGTSPGFSTS KTTASSIIVK 600
    AGYLLRGAHL DGADLHLTAD FNATTPIEVI GAPTGAKNLF VNGEKASHTV 650
    DKNGIWSSEV KYAAPEIKLP GLKDLDWKYL DTLPEIKSSY DDSAWVSADL 700
    PKTKNTHRPL DTPTSLYSSD YGFHTGYLIY RGHFVANGKE SEFFIRTQGG 750
    SAFGSSVWLN ETYLGSWTGA DYAMDGNSTY KLSQLESGKN YVITVVIDNL 800
    GLDENWTVGE ETMKNPRGIL SYKLSGQDAS AITWKLTGNL GGEDYQDKVR 850
    GPLNEGGLYA ERQGFHQPQP PSESWESGSP LEGLSKPGIG FYTAQFDLDL 900
    PKGWDVPLYF NFGNNTQAAR AQLYVNGYQY GKFTGNVGPQ TSFPVPEGIL 950
    NYRGTNYVAL SLWALESDGA KLGSFELSYT TPVLTGYGNV ESPEQPKYEQ 1000
    RKGAY 1005
    Length:1,005
    Mass (Da):109,870
    Last modified:March 3, 2009 - v1
    Checksum:iBAD290D2FA9EB003
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963474 Genomic DNA. Translation: EED54458.1.
    RefSeqiXP_002375730.1. XM_002375689.1.

    Genome annotation databases

    EnsemblFungiiCADAFLAT00003595; CADAFLAP00003595; CADAFLAG00003595.
    GeneIDi7918808.
    KEGGiafv:AFLA_017100.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963474 Genomic DNA. Translation: EED54458.1 .
    RefSeqi XP_002375730.1. XM_002375689.1.

    3D structure databases

    ProteinModelPortali B8N6V7.
    SMRi B8N6V7. Positions 41-1005.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5059.CADAFLAP00003595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFLAT00003595 ; CADAFLAP00003595 ; CADAFLAG00003595 .
    GeneIDi 7918808.
    KEGGi afv:AFLA_017100.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OMAi HNAPGTG.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiBGALA_ASPFN
    AccessioniPrimary (citable) accession number: B8N6V7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3