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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei298NucleophileSequence analysis1
Binding sitei364SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:AFLA_017100
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_017100

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039521619 – 1005Probable beta-galactosidase AAdd BLAST987

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi453N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi478N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi622N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi760N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi777N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi805N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi914N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiB8N6V7

Structurei

3D structure databases

ProteinModelPortaliB8N6V7
SMRiB8N6V7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi175 – 184Poly-Ala10

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922
OMAiHNAPGTG
OrthoDBiEOG092C0SLI

Family and domain databases

Gene3Di2.102.20.10, 1 hit
2.60.120.260, 3 hits
2.60.390.10, 1 hit
InterProiView protein in InterPro
IPR018954 Betagal_dom2
IPR037110 Betagal_dom2_sf
IPR025972 BetaGal_dom3
IPR036833 BetaGal_dom3_sf
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF10435 BetaGal_dom2, 1 hit
PF13363 BetaGal_dom3, 1 hit
PF13364 BetaGal_dom4_5, 2 hits
PF01301 Glyco_hydro_35, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SMARTiView protein in SMART
SM01029 BetaGal_dom2, 1 hit
SUPFAMiSSF117100 SSF117100, 1 hit
SSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B8N6V7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSVAAVA LLAAQAAGAS IKHRLNGFTI LEHPDPAKRD LLQDIVTWDD
60 70 80 90 100
KSLFINGERI MLFSGEVHPF RLPVPSLWLD IFHKIRALGF NCVSFYIDWA
110 120 130 140 150
LLEGKPGDYR AEGIFALEPF FDAAKEAGIY LIARPGSYIN AEVSGGGFPG
160 170 180 190 200
WLQRVNGTLR SSDEPFLKAT DNYIANAAAA VAKAQITNGG PVILYQPENE
210 220 230 240 250
YSGGCCGVKY PDADYMQYVM DQARKADIVV PFISNDASPS GHNAPGSGTG
260 270 280 290 300
AVDIYGHDSY PLGFDCANPS VWPEGKLPDN FRTLHLEQSP STPYSLLEFQ
310 320 330 340 350
AGAFDPWGGP GFEKCYALVN HEFSRVFYRN DLSFGVSTFN LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPITE TRNVTREKYS DIKLLANFVK ASPSYLTATP
410 420 430 440 450
RNLTTGVYTD TSDLAVTPLI GDSPGSFFVV RHTDYSSQES TSYKLKLPTS
460 470 480 490 500
AGNLTIPQLE GTLSLNGRDS KIHVVDYNVS GTNIIYSTAE VFTWKKFDGN
510 520 530 540 550
KVLVLYGGPK EHHELAIASK SNVTIIEGSD SGIVSTRKGS SVIIGWDVSS
560 570 580 590 600
TRRIVQVGDL RVFLLDRNSA YNYWVPELPT EGTSPGFSTS KTTASSIIVK
610 620 630 640 650
AGYLLRGAHL DGADLHLTAD FNATTPIEVI GAPTGAKNLF VNGEKASHTV
660 670 680 690 700
DKNGIWSSEV KYAAPEIKLP GLKDLDWKYL DTLPEIKSSY DDSAWVSADL
710 720 730 740 750
PKTKNTHRPL DTPTSLYSSD YGFHTGYLIY RGHFVANGKE SEFFIRTQGG
760 770 780 790 800
SAFGSSVWLN ETYLGSWTGA DYAMDGNSTY KLSQLESGKN YVITVVIDNL
810 820 830 840 850
GLDENWTVGE ETMKNPRGIL SYKLSGQDAS AITWKLTGNL GGEDYQDKVR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPQP PSESWESGSP LEGLSKPGIG FYTAQFDLDL
910 920 930 940 950
PKGWDVPLYF NFGNNTQAAR AQLYVNGYQY GKFTGNVGPQ TSFPVPEGIL
960 970 980 990 1000
NYRGTNYVAL SLWALESDGA KLGSFELSYT TPVLTGYGNV ESPEQPKYEQ

RKGAY
Length:1,005
Mass (Da):109,870
Last modified:March 3, 2009 - v1
Checksum:iBAD290D2FA9EB003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963474 Genomic DNA Translation: EED54458.1
RefSeqiXP_002375730.1, XM_002375689.1

Genome annotation databases

EnsemblFungiiEED54458; EED54458; AFLA_017100
GeneIDi7918808
KEGGiafv:AFLA_017100

Similar proteinsi

Entry informationi

Entry nameiBGALA_ASPFN
AccessioniPrimary (citable) accession number: B8N6V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 3, 2009
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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