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B8N417

- MNS1B_ASPFN

UniProt

B8N417 - MNS1B_ASPFN

Protein

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 2 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.By similarity

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Ca2+. Can also use Mg2+, but with lower efficiency By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei375 – 3751Proton donorBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113)
    Alternative name(s):
    Class I alpha-mannosidase 1B
    Man(9)-alpha-mannosidase 1B
    Gene namesi
    Name:mns1B
    Synonyms:msdS
    ORF Names:AFLA_033400
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 510489Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BPRO_0000394818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi332 ↔ 361By similarity
    Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi5059.CADAFLAP00002715.

    Structurei

    3D structure databases

    ProteinModelPortaliB8N417.
    SMRiB8N417. Positions 36-509.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181987.
    KOiK01230.
    OrthoDBiEOG7BP8BH.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B8N417-1 [UniParc]FASTAAdd to Basket

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    MHFSSLSLPL TALSLVTPSL AYPQFKFEQR VARSNSSESR ANAVKEAFVH    50
    AWDGYMQYAY PHDELHPISN GVGDSRNGWG ASAVDALSTA VIMGNETIVN 100
    QILDHIATID YSKTDDQVSL FETTIRYLGG MLSGYDLLKG PASNLVKDQA 150
    KVKTLLDQSQ NLADVLKFAF DTPSGIPYNN INITSHGNDG ATTNGLAVTG 200
    TLVLEWTRLS DLTGDTEYAQ LSQKAEDYLL NPSPKSAEPF EGLVGSHINI 250
    SNGAFADGQV SWNGGDDSFY EYLIKMYVYD PKRFSTYGDR WVKAAESSIK 300
    HLASHPEKRP DLTFLASYND GQYGLSSQHL TCFDGGSFLL GGTVLDRDDF 350
    IQFGLDLVKG CHETYNQTLT GIGPESFGWD PKNVPSDQKE LYERAGFYIS 400
    SGAYILRPEV IESFYYAWRI TGQEIYREWV WNAFVNINKY CRTDSGFAGL 450
    TNVNAANGGG RYDNQESFLF AEVLKYVYLT FAPDNEWQVQ RGKGNKFVYN 500
    TEAHPVRVAA 510
    Length:510
    Mass (Da):56,631
    Last modified:June 15, 2010 - v2
    Checksum:i5F24FEDB0B43F2BF
    GO

    Sequence cautioni

    The sequence EED56068.1 differs from that shown. Reason: Frameshift at position 233.
    The sequence EED56068.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963473 Genomic DNA. Translation: EED56068.1. Sequence problems.
    RefSeqiXP_002374850.1. XM_002374809.1.

    Genome annotation databases

    GeneIDi7910188.
    KEGGiafv:AFLA_033400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963473 Genomic DNA. Translation: EED56068.1 . Sequence problems.
    RefSeqi XP_002374850.1. XM_002374809.1.

    3D structure databases

    ProteinModelPortali B8N417.
    SMRi B8N417. Positions 36-509.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5059.CADAFLAP00002715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7910188.
    KEGGi afv:AFLA_033400.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181987.
    KOi K01230.
    OrthoDBi EOG7BP8BH.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

    Entry informationi

    Entry nameiMNS1B_ASPFN
    AccessioniPrimary (citable) accession number: B8N417
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 33 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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