B8N417 (MNS1B_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
EC=3.2.1.113

Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B

Gene names

Name:	mns1B
Synonyms:	msdS
ORF Names:	AFLA_033400

Organism

Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]

Taxonomic identifier

332952 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus flavus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Ca2+. Can also use Mg2+, but with lower efficiency By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasmic vesicle lumen By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.
Sequence caution	The sequence EED56068.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EED56068.1 differs from that shown. Reason: Frameshift at position 233.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 510	489	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B		PRO_0000394818
Sites
	Active site	375	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Glycosylation	366	1	N-linked (GlcNAc...) Potential
	Disulfide bond	332 ↔ 361		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B8N417 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 5F24FEDB0B43F2BF	FASTA	510	56,631
	10 20 30 40 50 60 MHFSSLSLPL TALSLVTPSL AYPQFKFEQR VARSNSSESR ANAVKEAFVH AWDGYMQYAY 70 80 90 100 110 120 PHDELHPISN GVGDSRNGWG ASAVDALSTA VIMGNETIVN QILDHIATID YSKTDDQVSL 130 140 150 160 170 180 FETTIRYLGG MLSGYDLLKG PASNLVKDQA KVKTLLDQSQ NLADVLKFAF DTPSGIPYNN 190 200 210 220 230 240 INITSHGNDG ATTNGLAVTG TLVLEWTRLS DLTGDTEYAQ LSQKAEDYLL NPSPKSAEPF 250 260 270 280 290 300 EGLVGSHINI SNGAFADGQV SWNGGDDSFY EYLIKMYVYD PKRFSTYGDR WVKAAESSIK 310 320 330 340 350 360 HLASHPEKRP DLTFLASYND GQYGLSSQHL TCFDGGSFLL GGTVLDRDDF IQFGLDLVKG 370 380 390 400 410 420 CHETYNQTLT GIGPESFGWD PKNVPSDQKE LYERAGFYIS SGAYILRPEV IESFYYAWRI 430 440 450 460 470 480 TGQEIYREWV WNAFVNINKY CRTDSGFAGL TNVNAANGGG RYDNQESFLF AEVLKYVYLT 490 500 510 FAPDNEWQVQ RGKGNKFVYN TEAHPVRVAA

References

[1]

"Genome sequence of Aspergillus flavus strain NRRL 3357." Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ963473 Genomic DNA. Translation: EED56068.1. Sequence problems.
RefSeq	XP_002374850.1. XM_002374809.1.
3D structure databases
ProteinModelPortal	B8N417.
SMR	B8N417. Positions 36-509.
ModBase	Search...
Protein-protein interaction databases
STRING	5059.CADAFLAP00002715.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFLAT00002715; CADAFLAP00002715; CADAFLAG00002715.
GeneID	7910188.
KEGG	afv:AFLA_033400.
Phylogenomic databases
eggNOG	NOG300315.
HOGENOM	HOG000181987.
KO	K01230.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS1B_ASPFN

Accession

Primary (citable) accession number: B8N417

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program