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B8N417 (MNS1B_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

EC=3.2.1.113
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene names
Name:mns1B
Synonyms:msdS
ORF Names:AFLA_033400
OrganismAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]
Taxonomic identifier332952 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Ca2+. Can also use Mg2+, but with lower efficiency By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasmic vesicle lumen By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Sequence caution

The sequence EED56068.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EED56068.1 differs from that shown. Reason: Frameshift at position 233.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasmic vesicle
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasmic membrane-bounded vesicle lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 510489Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
PRO_0000394818

Sites

Active site3751Proton donor By similarity

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2491N-linked (GlcNAc...) Potential
Glycosylation3661N-linked (GlcNAc...) Potential
Disulfide bond332 ↔ 361 By similarity

Sequences

Sequence LengthMass (Da)Tools
B8N417 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 5F24FEDB0B43F2BF

FASTA51056,631
        10         20         30         40         50         60 
MHFSSLSLPL TALSLVTPSL AYPQFKFEQR VARSNSSESR ANAVKEAFVH AWDGYMQYAY 

        70         80         90        100        110        120 
PHDELHPISN GVGDSRNGWG ASAVDALSTA VIMGNETIVN QILDHIATID YSKTDDQVSL 

       130        140        150        160        170        180 
FETTIRYLGG MLSGYDLLKG PASNLVKDQA KVKTLLDQSQ NLADVLKFAF DTPSGIPYNN 

       190        200        210        220        230        240 
INITSHGNDG ATTNGLAVTG TLVLEWTRLS DLTGDTEYAQ LSQKAEDYLL NPSPKSAEPF 

       250        260        270        280        290        300 
EGLVGSHINI SNGAFADGQV SWNGGDDSFY EYLIKMYVYD PKRFSTYGDR WVKAAESSIK 

       310        320        330        340        350        360 
HLASHPEKRP DLTFLASYND GQYGLSSQHL TCFDGGSFLL GGTVLDRDDF IQFGLDLVKG 

       370        380        390        400        410        420 
CHETYNQTLT GIGPESFGWD PKNVPSDQKE LYERAGFYIS SGAYILRPEV IESFYYAWRI 

       430        440        450        460        470        480 
TGQEIYREWV WNAFVNINKY CRTDSGFAGL TNVNAANGGG RYDNQESFLF AEVLKYVYLT 

       490        500        510 
FAPDNEWQVQ RGKGNKFVYN TEAHPVRVAA 

« Hide

References

[1]"Genome sequence of Aspergillus flavus strain NRRL 3357."
Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ963473 Genomic DNA. Translation: EED56068.1. Sequence problems.
RefSeqXP_002374850.1. XM_002374809.1.

3D structure databases

ProteinModelPortalB8N417.
SMRB8N417. Positions 36-509.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5059.CADAFLAP00002715.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7910188.
KEGGafv:AFLA_033400.

Phylogenomic databases

eggNOGNOG300315.
HOGENOMHOG000181987.
KOK01230.
OrthoDBEOG7BP8BH.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNS1B_ASPFN
AccessionPrimary (citable) accession number: B8N417
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: February 19, 2014
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries