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Protein

ATPase get3

Gene

get3

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62UniRule annotation1
Binding sitei244ATPUniRule annotation1
Binding sitei271ATPUniRule annotation1
Metal bindingi282Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi285Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 40ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase get3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:get3
ORF Names:AFLA_031310
OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Taxonomic identifieri332952 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000001875 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AFLA_031310

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003881881 – 339ATPase get3Add BLAST339

Proteomic databases

PRIDEiB8N3P7

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi5059.CADAFLAP00002506

Structurei

3D structure databases

ProteinModelPortaliB8N3P7
SMRiB8N3P7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000197637
KOiK01551
OrthoDBiEOG092C33XF

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

B8N3P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAVVQADD LMEPSLQSIV SQDTLRWIFV GGKGGVGKTT TSCSLAIQLA
60 70 80 90 100
KARKSVLLIS TDPAHNLSDA FGQKFGKEAR LVDGYTNLSA MEIDPNGSIQ
110 120 130 140 150
DLLASGEGQG DDPMAGLGVG NMMQDLAFSI PGVDEAMSFA EVLKQVKSLS
160 170 180 190 200
YEVIVFDTAP TGHTLRFLQF PTVLEKALAK LSQLSSQFGP MLNSILGSRG
210 220 230 240 250
GLPGGQNIDE LLQKMESLRE TISEVNTQFK NPDMTTFVCV CIAEFLSLYE
260 270 280 290 300
TERMIQELTS YNIDTHAIVV NQLLFPKQGS ECEQCNARRK MQKKYLEQIE
310 320 330
ELYEDFNVVR MPLLVEEVRG KEKLEKFSDM LIHPYVPPQ
Length:339
Mass (Da):37,417
Last modified:November 3, 2009 - v2
Checksum:iC9FCE4DF59D2FB24
GO

Sequence cautioni

The sequence EED55859 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ963473 Genomic DNA Translation: EED55859.1 Sequence problems.
RefSeqiXP_002374641.1, XM_002374600.1

Genome annotation databases

EnsemblFungiiEED55859; EED55859; AFLA_031310
GeneIDi7921698
KEGGiafv:AFLA_031310

Similar proteinsi

Entry informationi

Entry nameiGET3_ASPFN
AccessioniPrimary (citable) accession number: B8N3P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 3, 2009
Last modified: May 23, 2018
This is version 62 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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