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B8N0S9

- B8N0S9_ASPFN

UniProt

B8N0S9 - B8N0S9_ASPFN

Protein

Alpha-amylase

Gene

AFLA_026140

Organism
Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi142 – 1421Calcium 1UniRule annotation
    Metal bindingi183 – 1831Calcium 1; via carbonyl oxygenUniRule annotation
    Metal bindingi196 – 1961Calcium 1UniRule annotation
    Active sitei227 – 2271NucleophileUniRule annotation
    Metal bindingi227 – 2271Calcium 2UniRule annotation
    Metal bindingi231 – 2311Calcium 1; via carbonyl oxygenUniRule annotation
    Active sitei251 – 2511Proton donorUniRule annotation
    Metal bindingi251 – 2511Calcium 2UniRule annotation
    Sitei318 – 3181Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate catabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolismUniRule annotation

    Keywords - Ligandi

    CalciumUniRule annotation, Metal-bindingUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
    Gene namesi
    ORF Names:AFLA_026140Imported
    OrganismiAspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)Imported
    Taxonomic identifieri332952 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000001875: Unassembled WGS sequence

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi51 ↔ 59UniRule annotation
    Disulfide bondi171 ↔ 185UniRule annotation
    Disulfide bondi261 ↔ 304UniRule annotation
    Disulfide bondi461 ↔ 496UniRule annotation

    Keywords - PTMi

    Disulfide bondUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliB8N0S9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000165530.
    KOiK01176.
    OMAiNQTQVED.
    OrthoDBiEOG7RBZJ4.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013777. A-amylase_fun.
    IPR015340. A_amylase_DUF1966_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09260. DUF1966. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B8N0S9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMVAWWSLFL YGLQVAAPAL AATPADWRSQ SIYFLLTDRF ARTDGSTTAT    50
    CNTADRKYCG GTWQGIIDKL DYIQGMGFTA IWITPVTAQL PQTTAYGDAY 100
    HGYWQQDIYS LNENYGTADD LKALSSALHE RGMYLMVDVV ANHMGYDGAG 150
    SSVDYSVFKP FSSQDYFHPF CLIQNYEDQT QVEDCWLGDN TVSLPDLDTT 200
    KDVVKNEWYD WVGSLVSNYS IDGLRIDTVK HVQKDFWPGY NKAAGVYCIG 250
    EVLDGDPAYT CPYQNVMDGV LNYPIYYPLL NAFKSTSGSM NDLYNMINTV 300
    KSDCPDSTLL GTFVENHDNP RFASYTNDIA LAKNVAAFII LNDGIPIIYA 350
    GQEQHYAGGN DPANREATWL SGYPTDSELY KLIASANAIR NYAISKDTGF 400
    VTYKNWPIYK DDTTIAMRKG TDGSQIVTIL SNKGASGDSY TLSLSGAGYT 450
    AGQQLTEVIG CTTVTVGSDG NVPVPMAGGL PRVLYPTEKL AGSKICSSS 499
    Length:499
    Mass (Da):54,803
    Last modified:March 3, 2009 - v1
    Checksum:iB99E8A7D1CB8AAC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963473 Genomic DNA. Translation: EED55342.1.
    RefSeqiXP_002374124.1. XM_002374083.1.

    Genome annotation databases

    EnsemblFungiiCADAFLAT00001989; CADAFLAP00001989; CADAFLAG00001989.
    GeneIDi7919302.
    KEGGiafv:AFLA_026140.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ963473 Genomic DNA. Translation: EED55342.1 .
    RefSeqi XP_002374124.1. XM_002374083.1.

    3D structure databases

    ProteinModelPortali B8N0S9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFLAT00001989 ; CADAFLAP00001989 ; CADAFLAG00001989 .
    GeneIDi 7919302.
    KEGGi afv:AFLA_026140.

    Phylogenomic databases

    HOGENOMi HOG000165530.
    KOi K01176.
    OMAi NQTQVED.
    OrthoDBi EOG7RBZJ4.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013777. A-amylase_fun.
    IPR015340. A_amylase_DUF1966_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09260. DUF1966. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001024. Alph-amyl_fung. 1 hit.
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aspergillus flavus strain NRRL 3357."
      Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167Imported.

    Entry informationi

    Entry nameiB8N0S9_ASPFN
    AccessioniPrimary (citable) accession number: B8N0S9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 3, 2009
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3