B8MW97 (EGLB_ASPFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
probable endo-beta-1,4-glucanase B
Short name=Endoglucanase B
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase B
Cellulase B

Gene names

Name:	eglB
ORF Names:	AFLA_087870

Organism

Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) [Complete proteome]

Taxonomic identifier

332952 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus flavus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 333	316	probable endo-beta-1,4-glucanase B		PRO_0000394055
Sites
	Active site	160	1	Proton donor By similarity
	Active site	267	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	100	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	B8MW97 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: EA1271CB19A55C7A	FASTA	333	36,958
	10 20 30 40 50 60 MKFRNLFFAA VAGSAVAAPL AKEQKKRDSV FQWIGANESG AEFGENNLPG VWGTDYIFPD 70 80 90 100 110 120 VSAITTLIDK GMNIFRIQFK MERLVPDSMT GAYDEAYLQN LTTVVNAVTD AGVHAILDPH 130 140 150 160 170 180 NYGRFNGEIM STPSDFQTFW KNLAGQFQSN SLVIFDTNNE YHDMDQELVL NLNQAAIDGI 190 200 210 220 230 240 REAGATEQYI FVEGNSYTGA WTWTDVNDNM KNLEDPQDKI VYQMHQYLDS DGSGTSETCV 250 260 270 280 290 300 SGTIGQERVT SATQWLKDNK KVGIIGEFAG GNNDQCKTAV KGMLDYLAEN TDVWKGALWW 310 320 330 AAGPWWGDYM YSLEPPNGVA FTGMLDVLQA YLG

References

[1]

"Genome sequence of Aspergillus flavus strain NRRL 3357." Payne W.G.A., Dean R.A., Nierman W.C., Amedeo P., Caler E.G.A., Fedorova N.D., Maiti R., Joardar V., Inman J., Galinsky K.J., Yu J., Bhatnagar D., Cleveland T.E. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ963472 Genomic DNA. Translation: EED58088.1.
RefSeq	XP_002373700.1. XM_002373659.1.
3D structure databases
ProteinModelPortal	B8MW97.
SMR	B8MW97. Positions 30-332.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFLAT00001565; CADAFLAP00001565; CADAFLAG00001565.
GeneID	7917267.
KEGG	afv:AFLA_087870.
Phylogenomic databases
eggNOG	COG2730.
HOGENOM	HOG000111120.
KO	K01179.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EGLB_ASPFN

Accession

Primary (citable) accession number: B8MW97

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	March 3, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program