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B8MLU5 (LIPA_TALSN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase, mitochondrial

EC=2.8.1.8
Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase
Gene names
ORF Names:TSTA_101110
OrganismTalaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum) [Complete proteome]
Taxonomic identifier441959 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123

Subcellular location

Mitochondrion Potential HAMAP-Rule MF_03123.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion Potential
Chain35 – 422388Lipoyl synthase, mitochondrial HAMAP-Rule MF_03123
PRO_0000398294

Sites

Metal binding1461Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1511Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1571Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1771Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1811Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1841Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B8MLU5 [UniParc].

Last modified March 3, 2009. Version 1.
Checksum: 9F9C7823717AFD54

FASTA42246,127
        10         20         30         40         50         60 
MAASSTRLRC LYASSAPAWK KSPSQSIISL SRHYATTSST TPSLNPDESS SSSSSTIPKR 

        70         80         90        100        110        120 
RKTTTFRDKL NAGPSFADFV TGGNGNNASL DPEEAYALEK VMIPGPAGRK KEHTRLPSWL 

       130        140        150        160        170        180 
KTPIPDSTNY KRIKKDLRGL DLHTVCEEAR CPNISDCWGG SDKSAATATI MLMGDTCTRG 

       190        200        210        220        230        240 
CRFCSVKTLR TPGPLDPHEP ENTAEALSRW GLGDDLPDGG AHHFAETVIK IKQKAPGILV 

       250        260        270        280        290        300 
ECLTGDFAGD LDMVSLVAKS GLDVYAHNVE TVEALTPHVR DRRATFKQSL RVLEAAKRAK 

       310        320        330        340        350        360 
PSLITKTSMM LGFGETEDQL WDALRQLRAS NVDVVTFGQY MRPTKRHMAV HEYVTPDKFE 

       370        380        390        400        410        420 
LWRQRALEMG FLYVASGPLV RSSYKAGEAF IENVLKKRRG VGNTPGAEVA SAKDVPVDVL 


GK 

« Hide

References

[1]"Genome sequence of Talaromyces stipitatus strain ATCC 10500."
Fedorova N.D., Joardar V.S., Maiti R., Schobel S., Amedeo P., Galens K., Inman J.M., Galinsky K.J., White O.R., Whitty B.R., Wortman J.R., Nierman W.C.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EQ962658 Genomic DNA. Translation: EED13871.1.
RefSeqXP_002486109.1. XM_002486064.1.

3D structure databases

ProteinModelPortalB8MLU5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8101799.

Phylogenomic databases

OrthoDBEOG79KPR7.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_TALSN
AccessionPrimary (citable) accession number: B8MLU5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 3, 2009
Last modified: February 19, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways