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B8M990

- MAP22_TALSN

UniProt

B8M990 - MAP22_TALSN

Protein

Methionine aminopeptidase 2-2

Gene

TSTA_112180

Organism
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei216 – 2161SubstrateUniRule annotation
    Metal bindingi237 – 2371Divalent metal cation 1UniRule annotation
    Metal bindingi248 – 2481Divalent metal cation 1UniRule annotation
    Metal bindingi248 – 2481Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi317 – 3171Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei325 – 3251SubstrateUniRule annotation
    Metal bindingi350 – 3501Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi445 – 4451Divalent metal cation 1UniRule annotation
    Metal bindingi445 – 4451Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2-2UniRule annotation
    Short name:
    MetAP 2-2UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:TSTA_112180
    OrganismiTalaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)
    Taxonomic identifieri441959 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces
    ProteomesiUP000001745: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Methionine aminopeptidase 2-2PRO_0000407636Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB8M990.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi69 – 8517Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8M990-1 [UniParc]FASTAAdd to Basket

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    MGAKTYEGGD HHEDATNVLS TKSNAVGGKP RGANMLEDGD GEFGSDDDDD    50
    GGDGQDSSLA MVNPDDAAKP KKKKRSKKKK NNKKKSGAGA QRQTSPPRVP 100
    LSQLFPDGKY PIGQMVEVQD ENLRRTTDEE FRYLSRGTIT DDEALNDYRK 150
    AAEVHRQVRR WIHETIQPGS SLTELAVGIE DGVRALLEHQ GLEPGDSLKG 200
    GMGFPTGLAL NNCAAHYTPN PGQKDIILKT DDVLKIDFGV HVNGWIVDSA 250
    FTVTFDPVYD NLVAAVKDAT NTGLKCAGVD ARVGEIGGFI QEAMESYEVE 300
    INGKVYPVKS IRSITGHDIL RYRVHGGKQV PFVKSNDQTK MEEGEVFAIE 350
    TFGSTGKGYL RDGPGVYGYS KEPHAGNVHL PLASARALLK TINQNFGTIP 400
    FCRRYLDRLG IEKYLLGMNS LISHGIVQMY PPLVDIAGSY TAQFEHTILI 450
    NSSGNEIISR GDDY 464
    Length:464
    Mass (Da):50,618
    Last modified:March 3, 2009 - v1
    Checksum:i8C7B0975724386EC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ962655 Genomic DNA. Translation: EED17385.1.
    RefSeqiXP_002481377.1. XM_002481332.1.

    Genome annotation databases

    GeneIDi8098873.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ962655 Genomic DNA. Translation: EED17385.1 .
    RefSeqi XP_002481377.1. XM_002481332.1.

    3D structure databases

    ProteinModelPortali B8M990.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8098873.

    Phylogenomic databases

    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Talaromyces stipitatus strain ATCC 10500."
      Fedorova N.D., Joardar V.S., Maiti R., Schobel S., Amedeo P., Galens K., Inman J.M., Galinsky K.J., White O.R., Whitty B.R., Wortman J.R., Nierman W.C.
      Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006.

    Entry informationi

    Entry nameiMAP22_TALSN
    AccessioniPrimary (citable) accession number: B8M990
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3