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B8LUH2

- MAP21_TALSN

UniProt

B8LUH2 - MAP21_TALSN

Protein

Methionine aminopeptidase 2-1

Gene

TSTA_071420

Organism
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (03 Mar 2009)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei196 – 1961SubstrateUniRule annotation
    Metal bindingi216 – 2161Divalent metal cation 1UniRule annotation
    Metal bindingi227 – 2271Divalent metal cation 1UniRule annotation
    Metal bindingi227 – 2271Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi296 – 2961Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei304 – 3041SubstrateUniRule annotation
    Metal bindingi329 – 3291Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi424 – 4241Divalent metal cation 1UniRule annotation
    Metal bindingi424 – 4241Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2-1UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2-1UniRule annotation
    Short name:
    MetAP 2-1UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    ORF Names:TSTA_071420
    OrganismiTalaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) (Penicillium stipitatum)
    Taxonomic identifieri441959 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesTrichocomaceaeTalaromyces
    ProteomesiUP000001745: Unassembled WGS sequence

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Methionine aminopeptidase 2-1PRO_0000407614Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB8LUH2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi58 – 7518Lys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B8LUH2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAQADDELN KLRLNGQNGE AKEQVSASAV DTADNDDSED DEKEEEGGAE    50
    VAATEAAKKK KKRKPKKKKK GGAKKQSSPP RVPVSELFPN NQYPEGEIVE 100
    YKDENNYRTT NEEKRYLDRM NNDFLQEYRQ GAEVHRQVRQ YAQKNIKPGQ 150
    TLTEIAEGIE DAVRALTGHQ GLEEGDNIKG GMGFPCGLSI NHCAAHYTPN 200
    AGNKMVLQQG DVMKVDFGAH INGRIVDSAF TMTFDPVYDN LLTAVKEATN 250
    TGIREAGIDV RMSDIGAAIQ EVMESYEVEI NGTTYPVKAI RNLNGHNIDQ 300
    HVIHGGKSVP IVKGGDQTKM EEGEVFAIET FGSTGKGYVR EDMETSHYAK 350
    AQDAPNVSLR LSSAKNLLNV INKNFGTLPF CRRYLDRLGQ DKYLLGLNNL 400
    VSAGIVQDYP PLCDIKGSYT AQYEHTIVLR PTVKEVISRG DDY 443
    Length:443
    Mass (Da):48,965
    Last modified:March 3, 2009 - v1
    Checksum:i9838CC94CA68A14A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ962652 Genomic DNA. Translation: EED23745.1.
    RefSeqiXP_002341132.1. XM_002341091.1.

    Genome annotation databases

    GeneIDi8102525.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ962652 Genomic DNA. Translation: EED23745.1 .
    RefSeqi XP_002341132.1. XM_002341091.1.

    3D structure databases

    ProteinModelPortali B8LUH2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8102525.

    Phylogenomic databases

    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Talaromyces stipitatus strain ATCC 10500."
      Fedorova N.D., Joardar V.S., Maiti R., Schobel S., Amedeo P., Galens K., Inman J.M., Galinsky K.J., White O.R., Whitty B.R., Wortman J.R., Nierman W.C.
      Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006.

    Entry informationi

    Entry nameiMAP21_TALSN
    AccessioniPrimary (citable) accession number: B8LUH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3